Rapid one-step biotinylation of biological and non-biological surfaces

Henry, Stephen; Williams, Eleanor; Barr, Katie; Korchagina, Elena; Tuzikov, Alexandr; Ilyushina, Natalia; Abayzeed, Sidahmed A.; Webb, Kevin F.; Bovin, Nicolai

Abstract

We describe a rapid one-step method to biotinylate virtually any biological or non-biological surface. Contacting a solution of biotin-spacer-lipid constructs with a surface will form a coating within seconds on non-biological surfaces or within minutes on most biological membranes including membrane viruses. The resultant biotinylated surface can then be used to interact with avidinylated conjugates, beads, vesicles, surfaces or cells.

Citation

Henry, S., Williams, E., Barr, K., Korchagina, E., Tuzikov, A., Ilyushina, N., …Bovin, N. (2018). Rapid one-step biotinylation of biological and non-biological surfaces. Scientific Reports, 8(1), Article 2845. https://doi.org/10.1038/s41598-018-21186-3

• Journal Article Type: Article
• Acceptance Date: Jan 31, 2018
• Publication Date: Feb 12, 2018
• Deposit Date: Jun 6, 2018
• Publicly Available Date: Jun 6, 2018
• Journal: Scientific Reports
• Electronic ISSN: 2045-2322
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 8
• Issue: 1
• Article Number: 2845
• DOI: https://doi.org/10.1038/s41598-018-21186-3
• Public URL: https://nottingham-repository.worktribe.com/output/911223
• Publisher URL: https://doi.org/10.1038/s41598-018-21186-3