All Outputs (28)

Combined biolistic and cell penetrating peptide delivery for the development of scalable intradermal DNA vaccines (2024)
Journal Article

Physical-based gene delivery via biolistic methods (such as the Helios gene gun) involve precipitation of nucleic acids onto microparticles and direct transfection through cell membranes of exposed tissue (e.g. skin) by high velocity acceleration. Th... Read More about Combined biolistic and cell penetrating peptide delivery for the development of scalable intradermal DNA vaccines.

Effects of Microenvironment and Dosing on Efficiency of Enhanced Cell Penetrating Peptide Nonviral Gene Delivery (2024)
Journal Article

Transfection, defined as functional delivery of cell-internalized nucleic acids, is dependent on many factors linked to formulation, vector, cell type, and microenvironmental culture conditions. We previously developed a technology termed glycosamino... Read More about Effects of Microenvironment and Dosing on Efficiency of Enhanced Cell Penetrating Peptide Nonviral Gene Delivery.

Orally-delivered insulin-peptide nanocomplexes enhance transcytosis from cellular depots and improve diabetic blood glucose control (2023)
Journal Article

Insulin regulates blood glucose levels, and is the mainstay for the treatment of type-1 diabetes and type-2 when other drugs provide inadequate control. Therefore, effective oral Insulin delivery would be a significant advance in drug delivery. Herei... Read More about Orally-delivered insulin-peptide nanocomplexes enhance transcytosis from cellular depots and improve diabetic blood glucose control.

Direct contact-mediated non-viral gene therapy using thermo-sensitive hydrogel-coated dressings (2022)
Journal Article

Nanotechnologies are being increasingly applied as systems for peptide and nucleic acid macromolecule drug delivery. However systemic targeting of these, or efficient topical and localized delivery remains an issue. A controlled release system that c... Read More about Direct contact-mediated non-viral gene therapy using thermo-sensitive hydrogel-coated dressings.

RAPID INTRAOPERATIVE GENETIC AUGMENTATION OF AUTOGRAFTS FOR RECONSTRUCTIVE SURGERY OF SEVERE TRAUMA (2022)
Journal Article

Survivors with severe tissue injury have unmet clinical needs for better bone and soft tissue regeneration. Specifically they have progressive tissue loss due to inflammation, necrosis, infection and the development of critical size bone defects is a... Read More about RAPID INTRAOPERATIVE GENETIC AUGMENTATION OF AUTOGRAFTS FOR RECONSTRUCTIVE SURGERY OF SEVERE TRAUMA.

Development of a Gene-Activated Scaffold Incorporating Multifunctional Cell-Penetrating Peptides for pSDF-1α Delivery for Enhanced Angiogenesis in Tissue Engineering Applications (2022)
Journal Article

Non-viral gene delivery has become a popular approach in tissue engineering, as it permits the transient delivery of a therapeutic gene, in order to stimulate tissue repair. However, the efficacy of non-viral delivery vectors remains an issue. Our la... Read More about Development of a Gene-Activated Scaffold Incorporating Multifunctional Cell-Penetrating Peptides for pSDF-1α Delivery for Enhanced Angiogenesis in Tissue Engineering Applications.

Rapidly Transducing and Spatially Localized Magnetofection Using Peptide-Mediated Non-Viral Gene Delivery Based on Iron Oxide Nanoparticles (2020)
Journal Article

Non-viral delivery systems are generally of low efficiency, which limits their use in gene therapy and editing applications. We previously developed a technology termed glycosaminoglycan (GAG)-binding enhanced transduction (GET) to efficiently delive... Read More about Rapidly Transducing and Spatially Localized Magnetofection Using Peptide-Mediated Non-Viral Gene Delivery Based on Iron Oxide Nanoparticles.

Enhanced Cellular Transduction of Nanoparticles Resistant to Rapidly Forming Plasma Protein Coronas (2020)
Journal Article

© 2020 The Authors. Published by Wiley-VCH GmbH Nanoparticles (NPs) are increasingly being developed as biomedical platforms for drug/nucleic acid delivery and imaging. However, in biological fluids, NPs interact with a wide range of proteins that fo... Read More about Enhanced Cellular Transduction of Nanoparticles Resistant to Rapidly Forming Plasma Protein Coronas.

Efficient Delivery of Transducing Polymer Nanoparticles for Gene-mediated Induction of Osteogenesis for Bone Regeneration (2020)
Journal Article

Developing non-viral gene therapy vectors that both protect and functionally deliver nucleic acid cargoes will be vital if gene augmentation and editing strategies are to be effectively combined with advanced regenerative medicine approaches. Current... Read More about Efficient Delivery of Transducing Polymer Nanoparticles for Gene-mediated Induction of Osteogenesis for Bone Regeneration.

Genetically-programmed, mesenchymal stromal cell-laden & mechanically strong 3D bioprinted scaffolds for bone repair (2020)
Journal Article

Human-scale tissues with patterned vascular networks by additive manufacturing of sacrificial sugar-protein composites (2020)
Journal Article

© 2020 Combating necrosis, by supplying nutrients and removing waste, presents the major challenge for engineering large three-dimensional (3D) tissues. Previous elegant work used 3D printing with carbohydrate glass as a cytocompatible sacrificial te... Read More about Human-scale tissues with patterned vascular networks by additive manufacturing of sacrificial sugar-protein composites.

In vivo delivery of VEGF RNA and protein to increase osteogenesis and intraosseous angiogenesis (2019)
Journal Article

Deficient bone vasculature is a key component in pathological conditions ranging from developmental skeletal abnormalities to impaired bone repair. Vascularisation is dependent upon vascular endothelial growth factor (VEGF), which drives both angioge... Read More about In vivo delivery of VEGF RNA and protein to increase osteogenesis and intraosseous angiogenesis.

Molecular phenotyping of the surfaceome of migratory chondroprogenitors and mesenchymal stem cells using biotinylation, glycocapture and quantitative LC-MS/MS proteomic analysis (2019)
Journal Article

© 2019, The Author(s). The complement of cell surface proteins, collectively referred to as the surfaceome, is a useful indicator of normal differentiation processes, and the development of pathologies such as osteoarthritis (OA). We employed biochem... Read More about Molecular phenotyping of the surfaceome of migratory chondroprogenitors and mesenchymal stem cells using biotinylation, glycocapture and quantitative LC-MS/MS proteomic analysis.

Highly versatile cell-penetrating peptide loaded scaffold for efficient and localised gene delivery to multiple cell types: From development to application in tissue engineering (2019)
Journal Article

Gene therapy has recently come of age with seven viral vector-based therapies gaining regulatory approval in recent years. In tissue engineering, non-viral vectors are preferred over viral vectors, however, lower transfection efficiencies and difficu... Read More about Highly versatile cell-penetrating peptide loaded scaffold for efficient and localised gene delivery to multiple cell types: From development to application in tissue engineering.

Ex vivo MRI cell tracking of autologous mesenchymal stromal cells in an ovine osteochondral defect model (2019)
Journal Article

Background Osteochondral injuries represent a significant clinical problem requiring novel cell-based therapies to restore function of the damaged joint with the use of mesenchymal stromal cells (MSCs) leading research efforts. Pre-clinical studies... Read More about Ex vivo MRI cell tracking of autologous mesenchymal stromal cells in an ovine osteochondral defect model.

Discovery of peptide ligands targeting a specific ubiquitin-like domain– binding site in the deubiquitinase USP11 (2018)
Journal Article

© 2019 Spiliotopoulos et al. Published by The American Society for Biochemistry and Molecular Biology, Inc. Ubiquitin-specific proteases (USPs) reverse ubiquitination and regulate virtually all cellular processes. Defined noncatalytic domains in USP... Read More about Discovery of peptide ligands targeting a specific ubiquitin-like domain– binding site in the deubiquitinase USP11.

PEGylated enhanced cell penetrating peptide nanoparticles for lung gene therapy (2018)
Journal Article

The lung remains an attractive target for the gene therapy of monogenetic diseases such as cystic fibrosis (CF). Despite over 27 clinical trials, there are still very few gene therapy vectors that have shown any improvement in lung function; highligh... Read More about PEGylated enhanced cell penetrating peptide nanoparticles for lung gene therapy.

Post-modified polypeptides with UCST-type behavior for control of cell attachment in physiological conditions (2018)
Journal Article

Upper Critical Solution Temperature (UCST)-type thermally responsive polypeptides (TRPs) with phase transition temperatures around 37 ?C in phosphate-buffered saline (PBS) buffer (pH 7.4, 100 mM) were prepared from poly(L-ornithine) hydrobromide and... Read More about Post-modified polypeptides with UCST-type behavior for control of cell attachment in physiological conditions.

Osteogenic programming of human mesenchymal stem cells with highly efficient intracellular delivery of RUNX2 (2017)
Journal Article

Mesenchymal stem cells (MSCs) are being exploited in regenerative medicine due to their tri-lineage differentiation and immunomodulation activity. Currently, there are two major challenges when directing the differentiation of MSCs for therapeutic ap... Read More about Osteogenic programming of human mesenchymal stem cells with highly efficient intracellular delivery of RUNX2.

Controlled release of G AG-binding e nhanced t ransduction (GET) peptides for sustained and highly efficient intracellular delivery (2017)
Journal Article

Controlled release systems for therapeutic molecules are vital to allow the sustained local delivery of their activities which direct cell behaviour and enable novel regenerative strategies. Direct programming of cells using exogenously delivered tra... Read More about Controlled release of G AG-binding e nhanced t ransduction (GET) peptides for sustained and highly efficient intracellular delivery.