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Molecular phenotyping of the surfaceome of migratory chondroprogenitors and mesenchymal stem cells using biotinylation, glycocapture and quantitative LC-MS/MS proteomic analysis

Matta, Csaba; Boocock, David J.; Fellows, Christopher R.; Miosge, Nicolai; Dixon, James E.; Liddell, Susan; Smith, Julia; Mobasheri, Ali

Authors

Csaba Matta

David J. Boocock

Christopher R. Fellows

Nicolai Miosge

JAMES DIXON JAMES.DIXON@NOTTINGHAM.AC.UK
Associate Professor

Susan Liddell

Julia Smith

Ali Mobasheri



Abstract

© 2019, The Author(s). The complement of cell surface proteins, collectively referred to as the surfaceome, is a useful indicator of normal differentiation processes, and the development of pathologies such as osteoarthritis (OA). We employed biochemical and proteomic tools to explore the surfaceome and to define biomarkers in chondrogenic progenitor cells (CPC) derived from human OA knee articular cartilage. These cells have great therapeutic potential, but their unexplored biology limits their clinical application. We performed biotinylation combined with glycocapture and high throughput shotgun proteomics to define the surface proteome of human bone marrow mesenchymal stem cells (MSCs) and human CPCs. We prepared cell surfaceprotein-enriched fractions from MSCs and CPCs, and then a proteomic approach was used to compare and evaluate protein changes between undifferentiated MSCs and CPCs. 1256 proteins were identified in the study, of which 791 (63%) were plasma membrane, cell surface or extracellular matrix proteins. Proteins constituting the surfaceome were annotated and categorized. Our results provide, for the first time, a repository of quantitative proteomic data on the surfaceome of two closely related cell types relevant to cartilage biology and OA. These results may provide novel insights into the transformation of the surfaceome during chondrogenic differentiation and phenotypic changes during OA development.

Citation

Matta, C., Boocock, D. J., Fellows, C. R., Miosge, N., Dixon, J. E., Liddell, S., …Mobasheri, A. (2019). Molecular phenotyping of the surfaceome of migratory chondroprogenitors and mesenchymal stem cells using biotinylation, glycocapture and quantitative LC-MS/MS proteomic analysis. Scientific Reports, 9, Article 9018. https://doi.org/10.1038/s41598-019-44957-y

Journal Article Type Article
Acceptance Date Apr 24, 2019
Online Publication Date Jun 21, 2019
Publication Date Jun 21, 2019
Deposit Date Jul 12, 2019
Publicly Available Date Mar 29, 2024
Journal Scientific Reports
Print ISSN 2045-2322
Electronic ISSN 2045-2322
Publisher Nature Publishing Group
Peer Reviewed Peer Reviewed
Volume 9
Article Number 9018
DOI https://doi.org/10.1038/s41598-019-44957-y
Keywords Multidisciplinary
Public URL https://nottingham-repository.worktribe.com/output/2302748
Publisher URL https://www.nature.com/articles/s41598-019-44957-y

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