Csaba Matta
Molecular phenotyping of the surfaceome of migratory chondroprogenitors and mesenchymal stem cells using biotinylation, glycocapture and quantitative LC-MS/MS proteomic analysis
Matta, Csaba; Boocock, David J.; Fellows, Christopher R.; Miosge, Nicolai; Dixon, James E.; Liddell, Susan; Smith, Julia; Mobasheri, Ali
Authors
David J. Boocock
Christopher R. Fellows
Nicolai Miosge
JAMES DIXON JAMES.DIXON@NOTTINGHAM.AC.UK
Associate Professor
Susan Liddell
Julia Smith
Ali Mobasheri
Abstract
© 2019, The Author(s). The complement of cell surface proteins, collectively referred to as the surfaceome, is a useful indicator of normal differentiation processes, and the development of pathologies such as osteoarthritis (OA). We employed biochemical and proteomic tools to explore the surfaceome and to define biomarkers in chondrogenic progenitor cells (CPC) derived from human OA knee articular cartilage. These cells have great therapeutic potential, but their unexplored biology limits their clinical application. We performed biotinylation combined with glycocapture and high throughput shotgun proteomics to define the surface proteome of human bone marrow mesenchymal stem cells (MSCs) and human CPCs. We prepared cell surfaceprotein-enriched fractions from MSCs and CPCs, and then a proteomic approach was used to compare and evaluate protein changes between undifferentiated MSCs and CPCs. 1256 proteins were identified in the study, of which 791 (63%) were plasma membrane, cell surface or extracellular matrix proteins. Proteins constituting the surfaceome were annotated and categorized. Our results provide, for the first time, a repository of quantitative proteomic data on the surfaceome of two closely related cell types relevant to cartilage biology and OA. These results may provide novel insights into the transformation of the surfaceome during chondrogenic differentiation and phenotypic changes during OA development.
Citation
Matta, C., Boocock, D. J., Fellows, C. R., Miosge, N., Dixon, J. E., Liddell, S., …Mobasheri, A. (2019). Molecular phenotyping of the surfaceome of migratory chondroprogenitors and mesenchymal stem cells using biotinylation, glycocapture and quantitative LC-MS/MS proteomic analysis. Scientific Reports, 9, Article 9018. https://doi.org/10.1038/s41598-019-44957-y
Journal Article Type | Article |
---|---|
Acceptance Date | Apr 24, 2019 |
Online Publication Date | Jun 21, 2019 |
Publication Date | Jun 21, 2019 |
Deposit Date | Jul 12, 2019 |
Publicly Available Date | Mar 29, 2024 |
Journal | Scientific Reports |
Print ISSN | 2045-2322 |
Electronic ISSN | 2045-2322 |
Publisher | Nature Publishing Group |
Peer Reviewed | Peer Reviewed |
Volume | 9 |
Article Number | 9018 |
DOI | https://doi.org/10.1038/s41598-019-44957-y |
Keywords | Multidisciplinary |
Public URL | https://nottingham-repository.worktribe.com/output/2302748 |
Publisher URL | https://www.nature.com/articles/s41598-019-44957-y |
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Molecular phenotyping of the surfaceome of migratory chondroprogenitors and mesenchymal stem cells using biotinylation, glycocapture and quantitative LC-MS/MS proteomic analysis
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Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
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