Skip to main content

Research Repository

Advanced Search

Enhanced Cellular Transduction of Nanoparticles Resistant to Rapidly Forming Plasma Protein Coronas

Blokpoel Ferreras, Lia A.; Scott, Daniel; Vazquez Reina, Saul; Roach, Paul; Torres, Teobaldo E.; Goya, Gerardo F.; Shakesheff, Kevin M.; Dixon, James E.

Enhanced Cellular Transduction of Nanoparticles Resistant to Rapidly Forming Plasma Protein Coronas Thumbnail


Authors

Lia A. Blokpoel Ferreras

DANIEL SCOTT DANIEL.SCOTT@NOTTINGHAM.AC.UK
Nottingham Research Fellow

Saul Vazquez Reina

Paul Roach

Teobaldo E. Torres

Gerardo F. Goya

Kevin M. Shakesheff

JAMES DIXON JAMES.DIXON@NOTTINGHAM.AC.UK
Associate Professor



Abstract

© 2020 The Authors. Published by Wiley-VCH GmbH Nanoparticles (NPs) are increasingly being developed as biomedical platforms for drug/nucleic acid delivery and imaging. However, in biological fluids, NPs interact with a wide range of proteins that form a coating known as protein corona. Coronae can critically influence self-interaction and binding of other molecules, which can affect toxicity, promote cell activation, and inhibit general or specific cellular uptake. Glycosaminoglycan (GAG)-binding enhanced transduction (GET) is developed to efficiently deliver a variety of cargoes intracellularly; employing GAG-binding peptides, which promote cell targeting, and cell penetrating peptides (CPPs) which enhance endocytotic cell internalization. Herein, it is demonstrated that GET peptide coatings can mediate sustained intracellular transduction of magnetic NPs (MNPs), even in the presence of serum or plasma. NP colloidal stability, physicochemical properties, toxicity and cellular uptake are investigated. Using label-free snapshot proteomics, time-resolved profiles of human plasma coronas formed on functionalized GET-MNPs demonstrate that coronae quickly form (

Citation

Blokpoel Ferreras, L. A., Scott, D., Vazquez Reina, S., Roach, P., Torres, T. E., Goya, G. F., …Dixon, J. E. (2020). Enhanced Cellular Transduction of Nanoparticles Resistant to Rapidly Forming Plasma Protein Coronas. Advanced Biosystems, 4(10), Article 2000162. https://doi.org/10.1002/adbi.202000162

Journal Article Type Article
Acceptance Date Aug 18, 2020
Online Publication Date Sep 13, 2020
Publication Date 2020-10
Deposit Date Aug 25, 2020
Publicly Available Date Sep 13, 2020
Journal Advanced Biosystems
Print ISSN 2366-7478
Electronic ISSN 2366-7478
Peer Reviewed Peer Reviewed
Volume 4
Issue 10
Article Number 2000162
DOI https://doi.org/10.1002/adbi.202000162
Public URL https://nottingham-repository.worktribe.com/output/4851503
Publisher URL https://onlinelibrary.wiley.com/doi/10.1002/adbi.202000162

Files





You might also like



Downloadable Citations