Enhanced Cellular Transduction of Nanoparticles Resistant to Rapidly Forming Plasma Protein Coronas

Blokpoel Ferreras, Lia A.; Scott, Daniel; Vazquez Reina, Saul; Roach, Paul; Torres, Teobaldo E.; Goya, Gerardo F.; Shakesheff, Kevin M.; Dixon, James E.

Abstract

© 2020 The Authors. Published by Wiley-VCH GmbH Nanoparticles (NPs) are increasingly being developed as biomedical platforms for drug/nucleic acid delivery and imaging. However, in biological fluids, NPs interact with a wide range of proteins that form a coating known as protein corona. Coronae can critically influence self-interaction and binding of other molecules, which can affect toxicity, promote cell activation, and inhibit general or specific cellular uptake. Glycosaminoglycan (GAG)-binding enhanced transduction (GET) is developed to efficiently deliver a variety of cargoes intracellularly; employing GAG-binding peptides, which promote cell targeting, and cell penetrating peptides (CPPs) which enhance endocytotic cell internalization. Herein, it is demonstrated that GET peptide coatings can mediate sustained intracellular transduction of magnetic NPs (MNPs), even in the presence of serum or plasma. NP colloidal stability, physicochemical properties, toxicity and cellular uptake are investigated. Using label-free snapshot proteomics, time-resolved profiles of human plasma coronas formed on functionalized GET-MNPs demonstrate that coronae quickly form (

Citation

Blokpoel Ferreras, L. A., Scott, D., Vazquez Reina, S., Roach, P., Torres, T. E., Goya, G. F., Shakesheff, K. M., & Dixon, J. E. (2020). Enhanced Cellular Transduction of Nanoparticles Resistant to Rapidly Forming Plasma Protein Coronas. Advanced Biosystems, 4(10), Article 2000162. https://doi.org/10.1002/adbi.202000162

• Journal Article Type: Article
• Acceptance Date: Aug 18, 2020
• Online Publication Date: Sep 13, 2020
• Publication Date: 2020-10
• Deposit Date: Aug 25, 2020
• Publicly Available Date: Sep 13, 2020
• Journal: Advanced Biosystems
• Print ISSN: 2366-7478
• Electronic ISSN: 2366-7478
• Peer Reviewed: Peer Reviewed
• Volume: 4
• Issue: 10
• Article Number: 2000162
• DOI: https://doi.org/10.1002/adbi.202000162
• Public URL: https://nottingham-repository.worktribe.com/output/4851503
• Publisher URL: https://onlinelibrary.wiley.com/doi/10.1002/adbi.202000162