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Galacto-oligosaccharides fed during gestation increase Rotavirus A specific antibodies in sow colostrum, modulate the microbiome, and reduce infectivity in neonatal piglets in a commercial farm setting (2023)
Journal Article
Lee, A., Liang, L., Connerton, P. L., Connerton, I. F., & Mellits, K. H. (2023). Galacto-oligosaccharides fed during gestation increase Rotavirus A specific antibodies in sow colostrum, modulate the microbiome, and reduce infectivity in neonatal piglets in a commercial farm setting. Frontiers in Veterinary Science, 10, Article 1118302. https://doi.org/10.3389/fvets.2023.1118302

Introduction: Rotavirus A is a major cause of acute dehydrating diarrhea in neonatal pigs resulting in significant mortality, morbidity, reduced performance and economic loss. Commercially available prebiotic galacto-oligosaccharides are similar to t... Read More about Galacto-oligosaccharides fed during gestation increase Rotavirus A specific antibodies in sow colostrum, modulate the microbiome, and reduce infectivity in neonatal piglets in a commercial farm setting.

Common colonic community indicators of the suckling pig microbiota where diversity and abundance correlate with performance (2022)
Journal Article
Lee, A., Le Bon, M., Connerton, I. F., & Mellits, K. H. (2022). Common colonic community indicators of the suckling pig microbiota where diversity and abundance correlate with performance. FEMS Microbiology Ecology, 98(5), Article fiac048. https://doi.org/10.1093/femsec/fiac048

The primary objective of this study was to investigate if common colonic community indicators could be identified from the microbiota of 22-day-old suckling pigs in repeated small-scale trials. A total of three separate trials were conducted at diffe... Read More about Common colonic community indicators of the suckling pig microbiota where diversity and abundance correlate with performance.

Bacteriophage therapy to reduce colonization of campylobacter jejuni in broiler chickens before slaughter (2021)
Journal Article
D’angelantonio, D., Scattolini, S., Boni, A., Neri, D., Di Serafino, G., Connerton, P., …Aprea, G. (2021). Bacteriophage therapy to reduce colonization of campylobacter jejuni in broiler chickens before slaughter. Viruses, 13(8), Article 1428. https://doi.org/10.3390/v13081428

Campylobacteriosis is the most commonly reported gastrointestinal disease in humans. Campybacter jejuni is the main cause of the infection, and bacterial colonization in broiler chickens is widespread and difficult to prevent, leading to high risk of... Read More about Bacteriophage therapy to reduce colonization of campylobacter jejuni in broiler chickens before slaughter.

Acetate and auto-inducing peptide are independent triggers of quorum sensing in Lactobacillus plantarum (2021)
Journal Article
Meng, F., Lu, F., Du, H., Nie, T., Zhu, X., Connerton, I. F., …Lu, Y. (2021). Acetate and auto-inducing peptide are independent triggers of quorum sensing in Lactobacillus plantarum. Molecular Microbiology, 116(1), 298-310. https://doi.org/10.1111/mmi.14709

The synthesis of plantaricin in Lactobacillus plantarum is regulated by quorum sensing. However, the nature of the extra-cytoplasmic (EC) sensing domain of the histidine kinase (PlnB1) and the ability to recognize the auto-inducing peptide PlnA1 is n... Read More about Acetate and auto-inducing peptide are independent triggers of quorum sensing in Lactobacillus plantarum.

Bacteriophages to control multi-drug resistant enterococcus faecalis infection of dental root canals (2021)
Journal Article
El-Telbany, M., El-Didamony, G., Askora, A., Ariny, E., Abdallah, D., Connerton, I. F., & El-Shibiny, A. (2021). Bacteriophages to control multi-drug resistant enterococcus faecalis infection of dental root canals. Microorganisms, 9(3), Article 517. https://doi.org/10.3390/microorganisms9030517

© 2021 by the authors. Licensee MDPI, Basel, Switzerland. Phage therapy is an alternative treatment to antibiotics that can overcome multi-drug resistant bacteria. In this study, we aimed to isolate and characterize lytic bacteriophages targeted agai... Read More about Bacteriophages to control multi-drug resistant enterococcus faecalis infection of dental root canals.

Food biotechnology (2020)
Journal Article
Foster, T., Adams, G., di Bari, V., Connerton, I., Gould, J., Gouseti, O., …Yakubov, G. (2020). Food biotechnology. Current Opinion in Chemical Engineering, 30, 53-59. https://doi.org/10.1016/j.coche.2020.08.006

We investigate recent advances in the Chemical Engineering aspects of food structuring agents and macronutrients: carbohydrates, proteins and lipids, and also the fate of food upon ingestion. Prebiotic effects on host-microbe interactions enable impr... Read More about Food biotechnology.

Galacto-Oligosaccharides Modulate the Juvenile Gut Microbiome and Innate Immunity To Improve Broiler Chicken Performance (2020)
Journal Article
Connerton, I. F., Richards, P. J., Fish, N. M., Flaujac Lafontaine, G. M., Asiani, K., Connerton, P. L., …Richards, P. J. (2020). Galacto-Oligosaccharides Modulate the Juvenile Gut Microbiome and Innate Immunity To Improve Broiler Chicken Performance. mSystems, 5(1), Article e00827-19. https://doi.org/10.1128/msystems.00827-19

Copyright © 2020 Richards et al Improvements in growth performance and health are key goals in broiler chicken production. Inclusion of prebiotic galacto-oligosaccharides (GOS) in broiler feed enhanced the growth rate and feed conversion of chickens... Read More about Galacto-Oligosaccharides Modulate the Juvenile Gut Microbiome and Innate Immunity To Improve Broiler Chicken Performance.

Prebiotic Driven Increases in IL-17A Do Not Prevent Campylobacter jejuni Colonization of Chickens (2020)
Journal Article
Flaujac Lafontaine, G. M., Connerton, I. F., Richards, P. J., Fish, N. M., Connerton, P. L., Cummings, N. J., …Connerton, I. F. (2020). Prebiotic Driven Increases in IL-17A Do Not Prevent Campylobacter jejuni Colonization of Chickens. Frontiers in Microbiology, 10, Article 3030. https://doi.org/10.3389/fmicb.2019.03030

© Copyright © 2020 Flaujac Lafontaine, Richards, Connerton, O’Kane, Ghaffar, Cummings, Fish and Connerton. Worldwide Campylobacter jejuni is a leading cause of foodborne disease. Contamination of chicken meat with digesta from C. jejuni-positive bird... Read More about Prebiotic Driven Increases in IL-17A Do Not Prevent Campylobacter jejuni Colonization of Chickens.

Resistance mechanisms adopted by a Salmonella Typhimurium mutant against bacteriophage (2019)
Journal Article
Wang, C., Nie, T., Lin, F., Connerton, I. F., Lu, Z., Zhou, S., & Hang, H. (2019). Resistance mechanisms adopted by a Salmonella Typhimurium mutant against bacteriophage. Virus Research, 273, Article 197759. https://doi.org/10.1016/j.virusres.2019.197759

© 2019 Elsevier B.V. Bacteriophages have key roles in regulating bacterial populations in most habitats. A Salmonella Typhimurium mutant (N18) with impaired sensitivity to phage fmb-p1 was obtained and examined, the adsorption efficiency of fmb-p1 to... Read More about Resistance mechanisms adopted by a Salmonella Typhimurium mutant against bacteriophage.

Encapsulation of E. coli phage ZCEC5 in chitosan-alginate beads as a delivery system in phage therapy (2019)
Journal Article
Abdelsattar, A. S., Abdelrahmen, F., Dawoud, A., Connerton, I. F., & El-Shibiny, A. (2019). Encapsulation of E. coli phage ZCEC5 in chitosan-alginate beads as a delivery system in phage therapy. Applied Microbiology and Biotechnology, 9, Article 87. https://doi.org/10.1186/s13568-019-0810-9

© 2019, The Author(s). Bacteriophages can be used successfully to treat pathogenic bacteria in the food chain including zoonotic pathogens that colonize the intestines of farm animals. However, harsh gastric conditions of low pH and digestive enzyme... Read More about Encapsulation of E. coli phage ZCEC5 in chitosan-alginate beads as a delivery system in phage therapy.

Understanding “corruption” in regulatory agencies: The case of food inspection in Saudi Arabia (2019)
Journal Article
Al-Mutairi, S., Connerton, I., & Dingwall, R. (2019). Understanding “corruption” in regulatory agencies: The case of food inspection in Saudi Arabia. Regulation and Governance, 13(4), 507-519. https://doi.org/10.1111/rego.12247

Corruption is a relatively neglected topic in studies of regulatory agencies. The label is applied to a wide range of deviations from behavioral standards ultimately derived from Weber's account of the ideals of Prussian bureaucracy. This paper draws... Read More about Understanding “corruption” in regulatory agencies: The case of food inspection in Saudi Arabia.

Characterisation of aerotolerant forms of a robust chicken colonizing Campylobacter coli (2017)
Journal Article
O'Kane, P. M., & Connerton, I. F. (in press). Characterisation of aerotolerant forms of a robust chicken colonizing Campylobacter coli. Frontiers in Microbiology, 8, https://doi.org/10.3389/fmicb.2017.00513

Campylobacter contaminated poultry meat is a major source of human foodborne illness. Campylobacter coli strain OR12 is a robust colonizer of chickens that was previously shown to outcompete and displace other Campylobacter strains from the chicken’s... Read More about Characterisation of aerotolerant forms of a robust chicken colonizing Campylobacter coli.

The minor flagellin of Campylobacter jejuni (FlaB) confers defensive properties against bacteriophage infection (2016)
Journal Article
Lis, L., & Connerton, I. F. (in press). The minor flagellin of Campylobacter jejuni (FlaB) confers defensive properties against bacteriophage infection. Frontiers in Microbiology, 7, https://doi.org/10.3389/fmicb.2016.01908

A screen of bacteriophages infecting a panel of Campylobacter jejuni PT14 gene knock-out mutants identified a role for the minor flagellin encoded by the flaB gene, in the defense of the host against CP8unalikevirus bacteriophage CP_F1 infection. Ina... Read More about The minor flagellin of Campylobacter jejuni (FlaB) confers defensive properties against bacteriophage infection.

Pilot study of long-term anaesthesia in broiler chickens (2016)
Journal Article
O'Kane, P. M., Connerton, I. F., & White, K. L. (2016). Pilot study of long-term anaesthesia in broiler chickens. Veterinary Anaesthesia and Analgesia, 43(1), 72-75. https://doi.org/10.1111/vaa.12305

Objective: To provide stable anaesthesia of long duration in broiler chickens in order to perform a terminal caecal ligated loop procedure. Study design: Prospective experimental study. Animals: Seven clinically healthy broiler chickens (Gallus... Read More about Pilot study of long-term anaesthesia in broiler chickens.

Xylan degrading enzymes from fungal sources (2015)
Journal Article
Kirikyali, N., & Connerton, I. F. (2015). Xylan degrading enzymes from fungal sources. Journal of Biocatalysis and Biotransformation, 4(1), Article 118. https://doi.org/10.4172/10.4172/jpe.1000118

Fungi have the ability to degrade xylan as the major component of plant cell wall hemicellulose. Fungi have evolved batteries of xylanolytic enzymes that concertedly act to depolymerise xylan backbones decorated with variable carbohydrate branches. A... Read More about Xylan degrading enzymes from fungal sources.

Characterisation of a recombinant β-xylosidase (xylA) from Aspergillus oryzae expressed in Pichia pastoris (2014)
Journal Article
Kirikyali, N., Wood, J., & Connerton, I. F. (2014). Characterisation of a recombinant β-xylosidase (xylA) from Aspergillus oryzae expressed in Pichia pastoris. AMB Express, 4(68), https://doi.org/10.1186/s13568-014-0068-1

β-xylosidases catalyse the hydrolysis of short chain xylooligosaccharides from their non-reducing ends into xylose. In this study we report the heterologous expression of Aspergillus oryzae β-xylosidase (XylA) in Pichia pastoris under the control of... Read More about Characterisation of a recombinant β-xylosidase (xylA) from Aspergillus oryzae expressed in Pichia pastoris.

Food safety organisations in Saudi Arabia – organisational, historical and future analysis (2014)
Journal Article
Al-Mutairi, S., Connerton, I., & Dingwall, R. (2015). Food safety organisations in Saudi Arabia – organisational, historical and future analysis. Food Control, 47, 478-486. https://doi.org/10.1016/j.foodcont.2014.07.047

Food safety organisations around the world are increasingly seeking to develop public policies to protect society, improve the economy, and ensure food safety and quality, by reducing the risks of food-borne illnesses, commercial adulteration, and fo... Read More about Food safety organisations in Saudi Arabia – organisational, historical and future analysis.

Heterologous expression and kinetic characterisation of Neurospora crassa β-xylosidase in Pichia pastoris (2014)
Journal Article
Kirikyali, N., & Connerton, I. (2014). Heterologous expression and kinetic characterisation of Neurospora crassa β-xylosidase in Pichia pastoris. Enzyme and Microbial Technology, 57, 63-68. https://doi.org/10.1016/j.enzmictec.2014.02.002

To degrade plant hemicelluloses fungi employ β-xylosidases to hydrolyse xylooligosaccharides, released by endo-xylanases, into xylose. We have expressed the β-xylosidase from Neurospora crassa in Pichia pastoris under the control of alcohol oxidase 1... Read More about Heterologous expression and kinetic characterisation of Neurospora crassa β-xylosidase in Pichia pastoris.

A suggested classification for two groups of Campylobacter myoviruses (2013)
Journal Article
Javed, M. A., Ackermann, H.-W., Azeredo, J., Carvalho, C. M., Connerton, I., Evoy, S., …Kropinski, A. M. (2014). A suggested classification for two groups of Campylobacter myoviruses. Archives of Virology, 159(1), 181-190. https://doi.org/10.1007/s00705-013-1788-2

Most Campylobacter bacteriophages isolated to date have long contractile tails and belong to the family Myoviridae. Based on their morphology, genome size and endonuclease restriction profile, Campylobacter phages were originally divided into three g... Read More about A suggested classification for two groups of Campylobacter myoviruses.