Galacto-Oligosaccharides Modulate the Juvenile Gut Microbiome and Innate Immunity To Improve Broiler Chicken Performance

Richards, Philip J.; Connerton, Ian F.; Flaujac Lafontaine, Geraldine M.; Fish, Neville M.; Connerton, Phillippa L.; Asiani, Karishma; Liang, Lu; Liang, Lu; Asiani, Karishma; Connerton, Phillippa L.; Fish, Neville M.; Flaujac Lafontaine, Geraldine M.; Connerton, Ian; Richards, Philip J.

Abstract

Copyright © 2020 Richards et al Improvements in growth performance and health are key goals in broiler chicken production. Inclusion of prebiotic galacto-oligosaccharides (GOS) in broiler feed enhanced the growth rate and feed conversion of chickens relative to those obtained with a calorie-matched control diet. Comparison of the cecal microbiota identified key differences in abundances of Lactobacillus spp. Increased levels of Lactobacillus johnsonii in GOS-fed juvenile birds at the expense of Lactobacillus crispatus were linked to improved performance (growth rate and market weight). Investigation of the innate immune responses highlighted increases of ileal and cecal interleukin-17A (IL-17A) gene expression counterposed to a decrease in IL-10. Quantification of the autochthonous Lactobacillus spp. revealed a correlation between bird performance and L. johnsonii abundance. Shifts in the cecal populations of key Lactobacillus spp. of juvenile birds primed intestinal innate immunity without harmful pathogen challenge. IMPORTANCE Improvements in the growth rate of broiler chickens can be achieved through dietary manipulation of the naturally occurring bacterial populations while mitigating the withdrawal of antibiotic growth promoters. Prebiotic galactooligosaccharides (GOS) are manufactured as a by-product of dairy cheese production and can be incorporated into the diets of juvenile chickens to improve their health and performance. This study investigated the key mechanisms behind this progression and pinpointed L. johnsonii as a key species that facilitates the enhancements in growth rate and gut health. The study identified the relationships between the GOS diet, L. johnsonii intestinal populations, and cytokine immune effectors to improve growth.

Citation

Connerton, I. F., Richards, P. J., Fish, N. M., Flaujac Lafontaine, G. M., Asiani, K., Connerton, P. L., Liang, L., Liang, L., Connerton, P. L., Asiani, K., Fish, N. M., Flaujac Lafontaine, G. M., Connerton, I., & Richards, P. J. (2020). Galacto-Oligosaccharides Modulate the Juvenile Gut Microbiome and Innate Immunity To Improve Broiler Chicken Performance. mSystems, 5(1), Article e00827-19. https://doi.org/10.1128/msystems.00827-19

• Journal Article Type: Article
• Acceptance Date: Dec 16, 2019
• Online Publication Date: Jan 14, 2020
• Publication Date: Jan 14, 2020
• Deposit Date: Jan 8, 2020
• Publicly Available Date: Jan 8, 2020
• Journal: mSystems
• Electronic ISSN: 2379-5077
• Publisher: American Society for Microbiology
• Peer Reviewed: Peer Reviewed
• Volume: 5
• Issue: 1
• Article Number: e00827-19
• DOI: https://doi.org/10.1128/msystems.00827-19
• Public URL: https://nottingham-repository.worktribe.com/output/3591219
• Publisher URL: https://msystems.asm.org/content/5/1/e00827-19