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Galacto-oligosaccharides fed during gestation increase Rotavirus A specific antibodies in sow colostrum, modulate the microbiome, and reduce infectivity in neonatal piglets in a commercial farm setting

Lee, Adam; Liang, Lu; Connerton, Phillippa L.; Connerton, Ian F.; Mellits, Kenneth H.

Galacto-oligosaccharides fed during gestation increase Rotavirus A specific antibodies in sow colostrum, modulate the microbiome, and reduce infectivity in neonatal piglets in a commercial farm setting Thumbnail


Authors

Adam Lee

Lu Liang

Phillippa L. Connerton

IAN CONNERTON IAN.CONNERTON@NOTTINGHAM.AC.UK
Northern Foods Professor of Food Safety

KEN MELLITS KEN.MELLITS@NOTTINGHAM.AC.UK
Associate Professor



Abstract

Introduction: Rotavirus A is a major cause of acute dehydrating diarrhea in neonatal pigs resulting in significant mortality, morbidity, reduced performance and economic loss. Commercially available prebiotic galacto-oligosaccharides are similar to those of mammalian milk and stimulate the development of the microbiota and immune system in neonates. Little is known about the effects of supplementing sows' diets with galacto-oligosaccharides during gestation. This study aimed to determine if dietary galacto-oligosaccharide supplementation during gestation could improve immunity, reduce rotavirus infection and modulate the microbiota in sows and neonates in a commercial farm setting with confirmed natural endemic rotavirus challenge. Methods: In a randomized controlled trial, control sows received lactation diet with no galacto-oligosaccharide supplementation and test sows received lactation diet with 30 g/day galacto-oligosaccharide top-dressed into feed daily, seven days before farrowing. Colostrum was collected from sows 24 hours post-partum and tested for rotavirus specific antibodies. Fecal samples were collected from sows and piglets three days post-partum, tested for rotavirus A by qPCR and the microbiome composition assessed by 16s rRNA gene sequencing. Results: Supplementation with galacto-oligosaccharides during gestation significantly increased rotavirus-specific IgG and IgA in sow colostrum and reduced the number of rotavirus positive piglet fecal samples. Abundance of potential pathogens Treponema and Clostridiales were higher in fecal samples from non-galacto-oligosaccharide fed sows, their piglets and rotavirus positive samples. Discussion: This study demonstrates that galacto-oligosaccharide supplementation during gestation significantly increases rotavirus specific IgG and IgA in sow colostrum thereby reducing neonatal rotavirus infection and suppresses potential pathogenic bacteria in nursing sows and neonatal piglets.

Citation

Lee, A., Liang, L., Connerton, P. L., Connerton, I. F., & Mellits, K. H. (2023). Galacto-oligosaccharides fed during gestation increase Rotavirus A specific antibodies in sow colostrum, modulate the microbiome, and reduce infectivity in neonatal piglets in a commercial farm setting. Frontiers in Veterinary Science, 10, Article 1118302. https://doi.org/10.3389/fvets.2023.1118302

Journal Article Type Article
Acceptance Date Jan 17, 2023
Online Publication Date Feb 7, 2023
Publication Date Feb 7, 2023
Deposit Date Feb 9, 2023
Publicly Available Date Feb 9, 2023
Journal Frontiers in Veterinary Science
Print ISSN 2297-1769
Publisher Frontiers Media SA
Peer Reviewed Peer Reviewed
Volume 10
Article Number 1118302
DOI https://doi.org/10.3389/fvets.2023.1118302
Keywords Rotavirus, microbiota, pigs, galacto-oligosaccharides, antibodies, colostrum
Public URL https://nottingham-repository.worktribe.com/output/17082210
Publisher URL https://www.frontiersin.org/articles/10.3389/fvets.2023.1118302/full

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