Campylobacter Bacteriophage Infection at Refrigeration Temperatures

Hu, Yang; Al Shaaer, Bader; Liang, Lu; Connerton, Ian F.

Abstract

The application of bacteriophages to control foodborne bacterial pathogens in foods has gained traction in recent years. Poultry meat is a major source of Campylobacter jejuni, and a target for the application of bacteriophages. To offer the prospect of a post-harvest control measure, the bacteriophage must function at refrigeration temperatures, where C. jejuni does not grow but can survive. Here, we report actions of three classes of Campylobacter bacteriophage at 4 °C. The pre-incubation of broth cultures at 4 °C before a shift to 42 °C under conditions that support the growth of the host bacteria revealed differences in the time to lysis compared with cultures incubated at 42 °C. The pre-adsorption of the bacteriophage to a sub-population of bacteria is consistent with the observation of asynchronous infection. To ascertain whether the bacteriophages adsorb and infect (the commitment to replicate), we investigated bacteriophage transcription at 4 °C. RNA transcripts for all the bacteriophage host combinations were detected after 15 min, indicating that the interaction is not merely passive. Bacteriophages can infect C. jejuni at refrigeration temperatures, but the infection does not proceed to lysis in the absence of host cell division.

Citation

Hu, Y., Al Shaaer, B., Liang, L., & Connerton, I. F. (2023). Campylobacter Bacteriophage Infection at Refrigeration Temperatures. Applied Microbiology, 3(4), 1392-1406. https://doi.org/10.3390/applmicrobiol3040094

• Journal Article Type: Article
• Acceptance Date: Dec 11, 2023
• Online Publication Date: Dec 13, 2023
• Publication Date: 2023-12
• Deposit Date: Aug 29, 2024
• Publicly Available Date: Sep 26, 2024
• Journal: Applied Microbiology
• Electronic ISSN: 2673-8007
• Publisher: MDPI
• Peer Reviewed: Peer Reviewed
• Volume: 3
• Issue: 4
• Pages: 1392-1406
• DOI: https://doi.org/10.3390/applmicrobiol3040094
• Keywords: General Earth and Planetary Sciences; General Environmental Science
• Public URL: https://nottingham-repository.worktribe.com/output/28434071
• Publisher URL: https://www.mdpi.com/2673-8007/3/4/94