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Prebiotic Driven Increases in IL-17A Do Not Prevent Campylobacter jejuni Colonization of Chickens

Flaujac Lafontaine, Geraldine M.; Connerton, Ian F.; Richards, Philip J.; Fish, Neville M.; Connerton, Phillippa L.; Cummings, Nicola J.; O'Kane, Peter Michael; Ghaffar, Nacheervan M.; Ghaffar, Nacheervan M.; O�Kane, Peter M.; Cummings, Nicola J.; Connerton, Phillippa L.; Fish, Neville; Richards, Philip J.; Connerton, Ian F.; Flaujac Lafontaine, Geraldine M.

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Authors

Geraldine M. Flaujac Lafontaine

IAN CONNERTON IAN.CONNERTON@NOTTINGHAM.AC.UK
Northern Foods Professor of Food Safety

Philip J. Richards

Neville M. Fish

Phillippa L. Connerton

Nicola J. Cummings

Peter Michael O'Kane

Nacheervan M. Ghaffar

Nacheervan M. Ghaffar

Peter M. O�Kane

Nicola J. Cummings

Phillippa L. Connerton

Neville Fish

Philip J. Richards

IAN CONNERTON IAN.CONNERTON@NOTTINGHAM.AC.UK
Northern Foods Professor of Food Safety

Geraldine M. Flaujac Lafontaine



Abstract

© Copyright © 2020 Flaujac Lafontaine, Richards, Connerton, O’Kane, Ghaffar, Cummings, Fish and Connerton. Worldwide Campylobacter jejuni is a leading cause of foodborne disease. Contamination of chicken meat with digesta from C. jejuni-positive birds during slaughter and processing is a key route of transmission to humans through the food chain. Colonization of chickens with C. jejuni elicits host innate immune responses that may be modulated by dietary additives to provide a reduction in the number of campylobacters colonizing the gastrointestinal tract and thereby reduce the likelihood of human exposure to an infectious dose. Here we report the effects of prebiotic galacto-oligosaccharide (GOS) on broiler chickens colonized with C. jejuni when challenged at either an early stage in development at 6 days of age or 20 days old when campylobacters are frequently detected in commercial flocks. GOS-fed birds had increased growth performance, but the levels of C. jejuni colonizing the cecal pouches were unchanged irrespective of the age of challenge. Dietary GOS modulated the immune response to C. jejuni by increasing cytokine IL-17A expression at colonization. Correspondingly, reduced diversity of the cecal microbiota was associated with Campylobacter colonization in GOS-fed birds. In birds challenged at 6 days-old the reduction in microbial diversity was accompanied by an increase in the relative abundance of Escherichia spp. Whilst immuno-modulation of the Th17 pro-inflammatory response did not prevent C. jejuni colonization of the intestinal tract of broiler chickens, the study highlights the potential for combinations of prebiotics, and specific competitors (synbiotics) to engage with the host innate immunity to reduce pathogen burdens.

Citation

Flaujac Lafontaine, G. M., Connerton, I. F., Richards, P. J., Fish, N. M., Connerton, P. L., Cummings, N. J., …Connerton, I. F. (2020). Prebiotic Driven Increases in IL-17A Do Not Prevent Campylobacter jejuni Colonization of Chickens. Frontiers in Microbiology, 10, https://doi.org/10.3389/fmicb.2019.03030

Journal Article Type Article
Acceptance Date Dec 17, 2019
Online Publication Date Jan 14, 2020
Publication Date Jan 14, 2020
Deposit Date Dec 17, 2019
Publicly Available Date Dec 20, 2019
Journal Frontiers in Microbiology
Electronic ISSN 1664-302X
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 10
Article Number 3030
DOI https://doi.org/10.3389/fmicb.2019.03030
Keywords Microbiology (medical); Microbiology
Public URL https://nottingham-repository.worktribe.com/output/3591196
Publisher URL https://www.frontiersin.org/articles/10.3389/fmicb.2019.03030/full

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