Geraldine M. Flaujac Lafontaine
Prebiotic Driven Increases in IL-17A Do Not Prevent Campylobacter jejuni Colonization of Chickens
Flaujac Lafontaine, Geraldine M.; Connerton, Ian F.; Richards, Philip J.; Fish, Neville M.; Connerton, Phillippa L.; Cummings, Nicola J.; O'Kane, Peter Michael; Ghaffar, Nacheervan M.; Ghaffar, Nacheervan M.; O�Kane, Peter M.; Cummings, Nicola J.; Connerton, Phillippa L.; Fish, Neville; Richards, Philip J.; Connerton, Ian F.; Flaujac Lafontaine, Geraldine M.
Authors
Professor IAN CONNERTON IAN.CONNERTON@NOTTINGHAM.AC.UK
NORTHERN FOODS PROFESSOR OF FOOD SAFETY
Philip J. Richards
Neville M. Fish
Phillippa L. Connerton
Nicola J. Cummings
Peter Michael O'Kane
Nacheervan M. Ghaffar
Nacheervan M. Ghaffar
Peter M. O�Kane
Nicola J. Cummings
Phillippa L. Connerton
Neville Fish
Philip J. Richards
Professor IAN CONNERTON IAN.CONNERTON@NOTTINGHAM.AC.UK
NORTHERN FOODS PROFESSOR OF FOOD SAFETY
Geraldine M. Flaujac Lafontaine
Abstract
© Copyright © 2020 Flaujac Lafontaine, Richards, Connerton, O’Kane, Ghaffar, Cummings, Fish and Connerton. Worldwide Campylobacter jejuni is a leading cause of foodborne disease. Contamination of chicken meat with digesta from C. jejuni-positive birds during slaughter and processing is a key route of transmission to humans through the food chain. Colonization of chickens with C. jejuni elicits host innate immune responses that may be modulated by dietary additives to provide a reduction in the number of campylobacters colonizing the gastrointestinal tract and thereby reduce the likelihood of human exposure to an infectious dose. Here we report the effects of prebiotic galacto-oligosaccharide (GOS) on broiler chickens colonized with C. jejuni when challenged at either an early stage in development at 6 days of age or 20 days old when campylobacters are frequently detected in commercial flocks. GOS-fed birds had increased growth performance, but the levels of C. jejuni colonizing the cecal pouches were unchanged irrespective of the age of challenge. Dietary GOS modulated the immune response to C. jejuni by increasing cytokine IL-17A expression at colonization. Correspondingly, reduced diversity of the cecal microbiota was associated with Campylobacter colonization in GOS-fed birds. In birds challenged at 6 days-old the reduction in microbial diversity was accompanied by an increase in the relative abundance of Escherichia spp. Whilst immuno-modulation of the Th17 pro-inflammatory response did not prevent C. jejuni colonization of the intestinal tract of broiler chickens, the study highlights the potential for combinations of prebiotics, and specific competitors (synbiotics) to engage with the host innate immunity to reduce pathogen burdens.
Citation
Flaujac Lafontaine, G. M., Connerton, I. F., Richards, P. J., Fish, N. M., Connerton, P. L., Cummings, N. J., O'Kane, P. M., Ghaffar, N. M., Ghaffar, N. M., O’Kane, P. M., Cummings, N. J., Connerton, P. L., Fish, N., Richards, P. J., Flaujac Lafontaine, G. M., & Connerton, I. F. (2020). Prebiotic Driven Increases in IL-17A Do Not Prevent Campylobacter jejuni Colonization of Chickens. Frontiers in Microbiology, 10, Article 3030. https://doi.org/10.3389/fmicb.2019.03030
Journal Article Type | Article |
---|---|
Acceptance Date | Dec 17, 2019 |
Online Publication Date | Jan 14, 2020 |
Publication Date | Jan 14, 2020 |
Deposit Date | Dec 17, 2019 |
Publicly Available Date | Dec 20, 2019 |
Journal | Frontiers in Microbiology |
Electronic ISSN | 1664-302X |
Publisher | Frontiers Media |
Peer Reviewed | Peer Reviewed |
Volume | 10 |
Article Number | 3030 |
DOI | https://doi.org/10.3389/fmicb.2019.03030 |
Keywords | Microbiology (medical); Microbiology |
Public URL | https://nottingham-repository.worktribe.com/output/3591196 |
Publisher URL | https://www.frontiersin.org/articles/10.3389/fmicb.2019.03030/full |
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Prebiotic Driven Increases in IL-17A Do Not Prevent Campylobacter jejuni Colonization of Chickens
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Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
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