Encapsulation of E. coli phage ZCEC5 in chitosan-alginate beads as a delivery system in phage therapy

Abdelsattar, Abdallah S.; Abdelrahmen, Fatma; Dawoud, Alyaa; Connerton, Ian F.; El-Shibiny, Ayman

Abstract

© 2019, The Author(s). Bacteriophages can be used successfully to treat pathogenic bacteria in the food chain including zoonotic pathogens that colonize the intestines of farm animals. However, harsh gastric conditions of low pH and digestive enzyme activities affect phage viability, and accordingly reduce their effectiveness. We report the development of a natural protective barrier suitable for oral administration to farm animals that confers acid stability before functional release of bead-encapsulated phages. Escherichia coli bacteriophage ZSEC5 is rendered inactive at pH 2.0 but encapsulation in chitosan–alginate bead with a honey and gelatin matrix limited titer reductions to 1log10PFUmL−1. The encapsulated phage titers were stable upon storage in water but achieved near complete release over 4–5h in a simulated intestinal solution (0.1% bile salt, 0.4% pancreatin, 50mM KH2PO4 pH 7.5) at 37°C. Exposure of E. coli O157:H7 to the bead-encapsulated phage preparations produced a delayed response, reaching a maximal reductions of 4.2 to 4.8log10CFUmL−1 after 10h at 37°C under simulated intestinal conditions compared to a maximal reduction of 5.1log10CFUmL−1 at 3h for free phage applied at MOI = 1. Bead-encapsulation is a promising reliable and cost-effective method for the functional delivery of bacteriophage targeting intestinal bacteria of farm animals.

Citation

Abdelsattar, A. S., Abdelrahmen, F., Dawoud, A., Connerton, I. F., & El-Shibiny, A. (2019). Encapsulation of E. coli phage ZCEC5 in chitosan-alginate beads as a delivery system in phage therapy. Applied Microbiology and Biotechnology, 9, Article 87. https://doi.org/10.1186/s13568-019-0810-9

• Journal Article Type: Article
• Acceptance Date: Jun 6, 2019
• Online Publication Date: Jun 17, 2019
• Publication Date: Jun 17, 2019
• Deposit Date: Jun 5, 2019
• Publicly Available Date: Jun 18, 2020
• Journal: AMB Express
• Print ISSN: 0175-7598
• Electronic ISSN: 1432-0614
• Publisher: Springer Verlag
• Peer Reviewed: Peer Reviewed
• Volume: 9
• Article Number: 87
• DOI: https://doi.org/10.1186/s13568-019-0810-9
• Keywords: E-coli; Bacteriophage; Biocontrol; Phage encapsulation
• Public URL: https://nottingham-repository.worktribe.com/output/2144485
• Publisher URL: https://amb-express.springeropen.com/articles/10.1186/s13568-019-0810-9
• Additional Information: Received: 14 March 2019; Accepted: 6 June 2019; First Online: 17 June 2019; : Not applicable.; : Not applicable.; : The authors declare that they have no competing interests.