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The impact of surface chemistry modification on macrophage polarisation

Rostam, Hassan; Singh, Sonali; Salazar, Fabian; Magennis, Peter; Hook, Andrew L.; Singh, Taranjit; Vrana, Nihal; Alexander, Morgan R.; Ghaemmaghami, Amir M.

Authors

Hassan Rostam

Fabian Salazar

Peter Magennis

Andrew L. Hook

Taranjit Singh

Nihal Vrana



Abstract

Macrophages are innate immune cells that have a central role in combating infection and maintaining tissue homeostasis. They exhibit remarkable plasticity in response to environmental cues. At either end of a broad activation spectrum are pro-inflammatory (M1) and anti-inflammatory (M2) macrophages with distinct functional and phenotypical characteristics. Macrophages also play a crucial role in orchestrating immune responses to biomaterials used in the fabrication of implantable devices and drug delivery systems. To assess the impact of different surface chemistries on macrophage polarisation, human monocytes were cultured for 6 days on untreated hydrophobic polystyrene (PS) and hydrophilic O2 plasma-etched polystyrene (O2-PS40) surface. Our data clearly show that monocytes cultured on the hydrophilic O2-PS40 surface are polarised towards an M1-like phenotype, as evidenced by significantly higher expression of the pro-inflammatory transcription factors STAT1 and IRF5. By comparison, monocytes cultured on the hydrophobic PS surface exhibited an M2-like phenotype with high expression of mannose receptor (MR) and production of the anti-inflammatory cytokines IL-10 and CCL18. While the molecular basis of such different patterns of cell differentiation is yet to be fully elucidated, we hypothesise that it is due to the adsorption of different biomolecules on these surface chemistries. Indeed our surface characterisation data show quantitative and qualitative differences between the protein layers on that the O2-PS40 surface compared to PS surface which could be responsible for the observed differential macrophage polarisation on each surface.

Journal Article Type Article
Publication Date Nov 30, 2016
Journal Immunobiology
Print ISSN 0171-2985
Electronic ISSN 0171-2985
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 221
Issue 11
Pages 1237-1246
APA6 Citation Rostam, H., Singh, S., Salazar, F., Magennis, P., Hook, A. L., Singh, T., …Ghaemmaghami, A. M. (2016). The impact of surface chemistry modification on macrophage polarisation. Immunobiology, 221(11), 1237-1246. https://doi.org/10.1016/j.imbio.2016.06.010
DOI https://doi.org/10.1016/j.imbio.2016.06.010
Keywords Macrophage polarisation surface chemistry; Surface modification; M1; M2; O2 plasma etching; Macrophages; Foreign Body Response;
Publisher URL http://www.sciencedirect.com/science/article/pii/S0171298516300973
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0
Additional Information This article is maintained by: Elsevier; Article Title: The impact of surface chemistry modification on macrophage polarisation; Journal Title: Immunobiology; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.imbio.2016.06.010; Content Type: article; Copyright: © 2016 The Authors. Published by Elsevier GmbH.

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0





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