Yu Chao Zhu
Synergy between Toxoplasma gondii type I Δ GRA17 immunotherapy and PD-L1 checkpoint inhibition triggers the regression of targeted and distal tumors
Zhu, Yu Chao; Elsheikha, Hany M.; Wang, Jian Hua; Fang, Shuai; He, Jun Jun; Zhu, Xing Quan; Chen, Jia
Authors
Professor HANY ELSHEIKHA hany.elsheikha@nottingham.ac.uk
Professor of Interdisciplinary Parasitology
Jian Hua Wang
Shuai Fang
Jun Jun He
Xing Quan Zhu
Jia Chen
Abstract
Background: In this study, we hypothesize that the ability of the protozoan Toxoplasma gondii to modulate immune response within the tumor might improve the therapeutic effect of immune checkpoint blockade. We examined the synergetic therapeutic activity of attenuated T. gondii RH ΔGRA17 strain and programmed death ligand-1 (PD-L1) treatment on both targeted and distal tumors in mice.
Methods: The effects of administration of T. gondii RH ΔGRA17 strain on the tumor volume and survival rate of mice bearing flank B16-F10, MC38, or LLC tumors were studied. We characterized the effects of ΔGRA17 on tumor biomarkers’ expression, PD-L1 expression, immune cells infiltrating the tumors, and expression of immune-related genes by using immunohistochemistry, immunofluorescence, flow cytometry, NanoString platform, and real time quantitative PCR, respectively. The role of immune cells in the efficacy of ΔGRA17 plus PD-L1 blockade therapy was determined via depletion of immune cell subtypes.
Results: Treatment with T. gondii ΔGRA17 tachyzoites and anti-PD-L1 therapy significantly extended the survival of mice and suppressed tumor growth in preclinical mouse models of melanoma, Lewis lung carcinoma, and colon adenocarcinoma. Attenuation of the tumor growth was detected in the injected and distant tumors, which was associated with upregulation of innate and adaptive immune pathways. Complete regression of tumors was underpinned by late interferon-gamma-producing CD8+ cytotoxic T cells.
Conclusion: The results from these models indicate that intratumoral injection of ΔGRA17 induced a systemic effect, improved mouse immune response, and sensitized immunologically ‘cold’ tumors and rendered them sensitive to immune checkpoint blockade therapy.
Citation
Zhu, Y. C., Elsheikha, H. M., Wang, J. H., Fang, S., He, J. J., Zhu, X. Q., & Chen, J. (2021). Synergy between Toxoplasma gondii type I Δ GRA17 immunotherapy and PD-L1 checkpoint inhibition triggers the regression of targeted and distal tumors. Journal for ImmunoTherapy of Cancer, 9(11), Article e002970. https://doi.org/10.1136/jitc-2021-002970
Journal Article Type | Article |
---|---|
Acceptance Date | Oct 1, 2021 |
Online Publication Date | Nov 1, 2021 |
Publication Date | Nov 1, 2021 |
Deposit Date | Nov 5, 2021 |
Publicly Available Date | Nov 5, 2021 |
Journal | Journal for ImmunoTherapy of Cancer |
Electronic ISSN | 2051-1426 |
Publisher | BMJ |
Peer Reviewed | Peer Reviewed |
Volume | 9 |
Issue | 11 |
Article Number | e002970 |
DOI | https://doi.org/10.1136/jitc-2021-002970 |
Keywords | Cancer Research; Pharmacology; Oncology; Molecular Medicine; Immunology; Immunology and Allergy |
Public URL | https://nottingham-repository.worktribe.com/output/6613638 |
Publisher URL | https://jitc.bmj.com/content/9/11/e002970 |
Files
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Publisher Licence URL
https://creativecommons.org/licenses/by-nc/4.0/
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