Elisabeth C. Kugler
Cerebrovascular endothelial cells form transient Notch?dependent cystic structures in zebrafish
Kugler, Elisabeth C.; Lessen, Max; Daetwyler, Stephan; Chhabria, Karishma; Savage, Aaron M.; Silva, Vishmi; Plant, Karen; MacDonald, Ryan B.; Huisken, Jan; Wilkinson, Robert N.; Schulte?Merker, Stefan; Armitage, Paul; Chico, Timothy J.A.
Authors
Max Lessen
Stephan Daetwyler
Karishma Chhabria
Aaron M. Savage
Vishmi Silva
Karen Plant
Ryan B. MacDonald
Jan Huisken
Dr ROB WILKINSON Rob.Wilkinson@nottingham.ac.uk
Assistant Professor
Stefan Schulte?Merker
Paul Armitage
Timothy J.A. Chico
Contributors
Dr ROB WILKINSON Rob.Wilkinson@nottingham.ac.uk
Researcher
Abstract
We identify a novel endothelial membrane behaviour in transgenic zebrafish. Cerebral blood vessels extrude large transient spherical structures that persist for an average of 23 min before regressing into the parent vessel. We term these structures "kugeln", after the German for sphere. Kugeln are only observed arising from the cerebral vessels and are present as late as 28 days post fertilization. Kugeln do not communicate with the vessel lumen and can form in the absence of blood flow. They contain little or no cytoplasm, but the majority are highly positive for nitric oxide reactivity. Kugeln do not interact with brain lymphatic endothelial cells (BLECs) and can form in their absence, nor do they perform a scavenging role or interact with macrophages. Inhibition of actin polymerization, Myosin II, or Notch signalling reduces kugel formation, while inhibition of VEGF or Wnt dysregulation (either inhibition or activation) increases kugel formation. Kugeln represent a novel Notch-dependent NO-containing endothelial organelle restricted to the cerebral vessels, of currently unknown function
Journal Article Type | Article |
---|---|
Acceptance Date | May 3, 2019 |
Online Publication Date | Jun 18, 2019 |
Publication Date | Aug 1, 2019 |
Deposit Date | Nov 12, 2019 |
Journal | EMBO reports |
Print ISSN | 1469-221X |
Electronic ISSN | 1469-3178 |
Publisher | EMBO Press |
Peer Reviewed | Peer Reviewed |
Volume | 20 |
Issue | 8 |
DOI | https://doi.org/10.15252/embr.201847047 |
Keywords | Genetics; Biochemistry; Molecular Biology |
Public URL | https://nottingham-repository.worktribe.com/output/3230363 |
Publisher URL | https://www.embopress.org/doi/abs/10.15252/embr.201847047 |
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