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Proteomic profiling of mouse liver following acute Toxoplasma gondii infection

He, Jun-Jun; Ma, Jun; Elsheikha, Hany M.; Song, Hui-Qun; Zhou, Dong-Hui; Zhu, Xing-Quan

Authors

Jun-Jun He

Jun Ma

Hany M. Elsheikha

Hui-Qun Song

Dong-Hui Zhou

Xing-Quan Zhu



Abstract

Toxoplasma gondii remains a global public health problem. However, its pathophysiology is still not-completely understood particularly the impact of infection on host liver metabolism. We performed iTRAQ-based proteomic analysis to evaluate early liver protein responses in BALB/c mice following infection with T. gondii PYS strain (genotype ToxoDB#9) infection. Our data revealed modification of protein expression in key metabolic pathways, as indicated by the upregulation of immune response and downregulation of mitochondrial respiratory chain, and the metabolism of fatty acids, lipids and xenobiotics. T. gondii seems to hijack host PPAR signaling pathway to downregulate the metabolism of fatty acids, lipids and energy in the liver. The metabolism of over 400 substances was affected by the downregulation of genes involved in xenobiotic metabolism. The top 10 transcription factors used by upregulated genes were Stat2, Stat1, Irf2, Irf1, Sp2, Egr1, Stat3, Klf4, Elf1 and Gabpa, while the top 10 transcription factors of downregulated genes were Hnf4A, Ewsr1, Fli1, Hnf4g, Nr2f1, Pparg, Rxra, Hnf1A, Foxa1 and Foxo1. These findings indicate global reprogramming of the metabolism of the mouse liver after acute T. gondii infection. Functional characterization of the altered proteins may enhance understanding of the host responses to T. gondii infection and lead to the identification of new therapeutic targets.

Journal Article Type Article
Publication Date Mar 22, 2016
Electronic ISSN 1932-6203
Publisher Public Library of Science
Peer Reviewed Peer Reviewed
Volume 11
Issue 3
Article Number e0152022
Pages 1-15
Institution Citation He, J., Ma, J., Elsheikha, H. M., Song, H., Zhou, D., & Zhu, X. (2016). Proteomic profiling of mouse liver following acute Toxoplasma gondii infection. PLoS ONE, 11(3), 1-15. doi:10.1371/journal.pone.0152022
DOI https://doi.org/10.1371/journal.pone.0152022
Publisher URL https://doi.org/10.1371/journal.pone.0152022
PMID 00037269

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