Dr ADAM BLANCHARD's Outputs (43)

In vitro determination of essential genes required by Streptococcus uberis to grow in a complex biological media relating to intramammary infection (2025)
Journal Article
Blanchard, A. M., & Leigh, J. A. (2025). In vitro determination of essential genes required by Streptococcus uberis to grow in a complex biological media relating to intramammary infection. Microbial Genomics, 11(6), Article 001425. https://doi.org/10.1099/mgen.0.001425

Streptococcus uberis is a leading cause of bovine mastitis, a disease of significant economic impact to the dairy industry. Understanding the genetic factors essential for bacterial survival in milk and during intramammary infection is crucial for de... Read More about In vitro determination of essential genes required by Streptococcus uberis to grow in a complex biological media relating to intramammary infection.

Progressive evolution of Streptococcus equi from Streptococcus equi subsp. zooepidemicus and adaption to equine hosts (2025)
Journal Article
Wilson, H. J., Dong, J., van Tonder, A. J., Ruis, C., Lefrancq, N., McGlennon, A., Bustos, C., Frosth, S., Léon, A., Blanchard, A. M., Holden, M., Waller, A. S., & Parkhill, J. (2025). Progressive evolution of Streptococcus equi from Streptococcus equi subsp. zooepidemicus and adaption to equine hosts. Microbial Genomics, 11(3), Article 001366. https://doi.org/10.1099/mgen.0.001366

Streptococcus equi subsp. equi causes the equine respiratory disease ‘strangles’, which is highly contagious, debilitating and costly to the equine industry. S. equi emerged from the ancestral Streptococcus equi subsp. zooepidemicus and continues to... Read More about Progressive evolution of Streptococcus equi from Streptococcus equi subsp. zooepidemicus and adaption to equine hosts.

Whole-Genome Sequencing Analysis Revealed High Genomic Variability, Recombination Events and Mobile Genetic Elements in Streptococcus uberis Strains Isolated from Bovine Mastitis in Colombian Dairy Herds (2025)
Journal Article
Rios Agudelo, P. A., Reyes Vélez, J., Olivera Angel, M., Blanchard, A. M., Cuesta Astroz, Y., Caraballo Guzmán, A., & Torres Lindarte, G. (in press). Whole-Genome Sequencing Analysis Revealed High Genomic Variability, Recombination Events and Mobile Genetic Elements in Streptococcus uberis Strains Isolated from Bovine Mastitis in Colombian Dairy Herds. Antibiotics, 14(3), Article 297. https://doi.org/10.3390/antibiotics14030297

Introduction: Streptococcus uberis is a poorly controlled cause of bovine intramammary infections and a common motivation for the use antibiotics in dairy farms worldwide. Therefore, studying the genomic characteristics of this pathogen is fundamenta... Read More about Whole-Genome Sequencing Analysis Revealed High Genomic Variability, Recombination Events and Mobile Genetic Elements in Streptococcus uberis Strains Isolated from Bovine Mastitis in Colombian Dairy Herds.

A rapid genome-proteome approach to identify rate-limiting steps in the butyrate production pathway in probiotic Clostridium butyricum, CBM588 (2025)
Journal Article
Wood, L., Omorotionmwan, B. B., Blanchard, A. M., Dowle, A., Tooth, D., Bailey, T. S., & Griffin, R. (2025). A rapid genome-proteome approach to identify rate-limiting steps in the butyrate production pathway in probiotic Clostridium butyricum, CBM588. Anaerobe, 92, Article 102940. https://doi.org/10.1016/j.anaerobe.2025.102940

Objectives: Clostridium butyricum ferments non-digested dietary fibre in the colon to produce butyric acid. Butyrate is a four-carbon, short-chain fatty acid (SCFA) which has multiple health benefits. Many microbial products of pharmaceutical or indu... Read More about A rapid genome-proteome approach to identify rate-limiting steps in the butyrate production pathway in probiotic Clostridium butyricum, CBM588.

Understanding the Molecular Interactions Between Influenza A Virus and Streptococcus Proteins in Co-Infection: A Scoping Review (2025)
Journal Article
Alshammari, A. K., Maina, M., Blanchard, A. M., Daly, J. M., & Dunham, S. P. (2025). Understanding the Molecular Interactions Between Influenza A Virus and Streptococcus Proteins in Co-Infection: A Scoping Review. Pathogens, 14(2), Article 114. https://doi.org/10.3390/pathogens14020114

Influenza A virus infections are known to predispose infected individuals to bacterial infections of the respiratory tract that result in co-infection with severe disease outcomes. Co-infections involving influenza A viruses and streptococcus bacteri... Read More about Understanding the Molecular Interactions Between Influenza A Virus and Streptococcus Proteins in Co-Infection: A Scoping Review.

Euclid preparation: LV. Exploring the properties of proto-clusters in the Simulated Euclid Wide Survey (2025)
Journal Article
Euclid Collaboration, Böhringer, H., Chon, G., Cucciati, O., Dannerbauer, H., Bolzonella, M., De Lucia, G., Cappi, A., Moscardini, L., Giocoli, C., Castignani, G., Hatch, N. A., Andreon, S., Bañados, E., Ettori, S., Fontanot, F., Gully, H., Hirschmann, M., Maturi, M., Mei, S., …Verza, G. (2025). Euclid preparation: LV. Exploring the properties of proto-clusters in the Simulated Euclid Wide Survey. Astronomy and Astrophysics, 693, Article A59. https://doi.org/10.1051/0004-6361/202451683

Galaxy proto-clusters are receiving increased interest since most of the processes shaping the structure of clusters of galaxies and their galaxy population happen at the early stages of their formation. The Euclid Survey will provide a unique opport... Read More about Euclid preparation: LV. Exploring the properties of proto-clusters in the Simulated Euclid Wide Survey.

Draft genomes of two contemporary strains of Babesia divergens (2024)
Journal Article
Apaa, T. T., Jones, B. P., Blanchard, A. M., & Johnson, N. (2025). Draft genomes of two contemporary strains of Babesia divergens. Microbiology Resource Announcements, 14(1), Article e00898-24. https://doi.org/10.1128/mra.00898-24

Babesia divergens, a major cause of bovine babesiosis with zoonotic potential, was analyzed through genomes Bdiv23B and Bdiv24B following Illumina sequencing of DNA extracted from PCR-positive cattle blood. The genomes comprised 3888 and 4032 predict... Read More about Draft genomes of two contemporary strains of Babesia divergens.

PIMMS-Dash: Accessible analysis, interrogation, and visualisation of high-throughput transposon insertion sequencing (TIS) data (2024)
Journal Article
Blanchard, A. M., Taylor, A., Warry, A., Shephard, F., Curwen, A., Leigh, J. A., Emes, R. D., & Egan, S. A. (2024). PIMMS-Dash: Accessible analysis, interrogation, and visualisation of high-throughput transposon insertion sequencing (TIS) data. Computational and Structural Biotechnology Journal, 23, 3780-3783. https://doi.org/10.1016/j.csbj.2024.10.025

Motivation: Current methods for visualising and interrogating high-throughput transposon insertion mutagenesis sequencing (TIS) data requires a significant time investment in learning bioinformatics, often producing static figures that do not facilit... Read More about PIMMS-Dash: Accessible analysis, interrogation, and visualisation of high-throughput transposon insertion sequencing (TIS) data.

Multiple novel caliciviruses identified from stoats (Mustela erminea) in the United Kingdom (2024)
Journal Article
Hinds, J., Apaa, T., Parry, R. H., Withers, A. J., MacKenzie, L., Staley, C., Morrison, J., Bennett, M., Bremner-Harrison, S., Chadwick, E. A., Hailer, F., Harrison, S. W., Lambin, X., Loose, M., Mathews, F., Tarlinton, R., & Blanchard, A. (2024). Multiple novel caliciviruses identified from stoats (Mustela erminea) in the United Kingdom. Access Microbiology, 6(7), Article 000813.v4. https://doi.org/10.1099/acmi.0.000813.v4

The Caliciviridae family, comprising positive-sense RNA viruses, is characterised by its non-enveloped, small virions, broad host range, and notable tendency for host switching. These viruses are primarily associated with gastroenteric disease, thoug... Read More about Multiple novel caliciviruses identified from stoats (Mustela erminea) in the United Kingdom.

Version 3: Multiple novel caliciviruses identified from stoats (Mustela erminea) in the United Kingdom (2024)
Preprint / Working Paper
Hinds, J., Apaa, T., Parry, R. H., Withers, A. J., MacKenzie, L., Staley, C., Morrison, J., Bennett, M., Bremner-Harrison, S., Chadwick, E. A., Hailer, F., Harrison, S. W., Lambin, X., Loose, M., Mathews, F., Tarlinton, R., & Blanchard, A. (2024). Version 3: Multiple novel caliciviruses identified from stoats (Mustela erminea) in the United Kingdom

The Caliciviridae family, comprising positive-sense RNA viruses, is characterised by its non-enveloped, small virions, broad host range, and notable tendency for host switching. These viruses are primarily associated with gastroenteric disease, thoug... Read More about Version 3: Multiple novel caliciviruses identified from stoats (Mustela erminea) in the United Kingdom.

Modelling the impact of wastewater flows and management practices on antimicrobial resistance in dairy farms (2024)
Journal Article
Todman, H., Helliwell, R., King, L., Blanchard, A., Gray-Hammerton, C. J., Hooton, S. P., Baker, M., Margerison, J., Wilson, P., Dodd, C. E. R., Morris, C., Raman, S., Hudson, C., Kreft, J.-U., Hobman, J. L., Kypraios, T., & Stekel, D. J. (2024). Modelling the impact of wastewater flows and management practices on antimicrobial resistance in dairy farms. npj Antimicrobials and Resistance, 2(1), Article 13. https://doi.org/10.1038/s44259-024-00029-4

Dairy slurry is a major source of environmental contamination with antimicrobial resistant genes and bacteria. We developed mathematical models and conducted on-farm research to explore the impact of wastewater flows and management practices on antim... Read More about Modelling the impact of wastewater flows and management practices on antimicrobial resistance in dairy farms.

PIMMS-Dash: Accessible analysis, interrogation, and visualisation of high-throughput transposon insertion sequencing (TIS) data (2024)
Preprint / Working Paper
Blanchard, A. M., Taylor, A., Warry, A., Shephard, F., Curwen, A., Leigh, J. A., Emes, R. D., & Egan, S. A. (2024). PIMMS-Dash: Accessible analysis, interrogation, and visualisation of high-throughput transposon insertion sequencing (TIS) data

Motivation Current methods for visualising and interrogating high-throughput transposon insertion mutagenesis sequencing (TIS) data requires a significant time investment in learning bioinformatics, often producing static figures that do not facilita... Read More about PIMMS-Dash: Accessible analysis, interrogation, and visualisation of high-throughput transposon insertion sequencing (TIS) data.

A core genome multi-locus sequence typing scheme for Streptococcus uberis: an evolution in typing a genetically diverse pathogen (2024)
Journal Article
Whiley, D., Jolley, K., Blanchard, A., Coffey, T., & Leigh, J. (2024). A core genome multi-locus sequence typing scheme for Streptococcus uberis: an evolution in typing a genetically diverse pathogen. Microbial Genomics, 10(3), https://doi.org/10.1099/mgen.0.001225

Streptococcus uberis is a globally endemic and poorly controlled cause of bovine mastitis impacting the sustainability of the modern dairy industry. A core genome was derived from 579 newly sequenced S. uberis isolates, along with 305 publicly availa... Read More about A core genome multi-locus sequence typing scheme for Streptococcus uberis: an evolution in typing a genetically diverse pathogen.

Lack of detection of SARS-CoV-2 in wildlife from Kerala, India in 2020–21 (2024)
Journal Article
Zachariah, A., Krishnankutty, S. P., Manazhi, J., Omanakuttan, V., Santosh, S., Blanchard, A., & Tarlinton, R. (2024). Lack of detection of SARS-CoV-2 in wildlife from Kerala, India in 2020–21. Access Microbiology, 6(1), Article 000686. https://doi.org/10.1099/acmi.0.000686.v3

Spillover of SARS-CoV-2 into a variety of wild and domestic animals has been an ongoing feature of the human pandemic. The establishment of a new reservoir in white-tailed deer in North America and increasing divergence of the viruses circulating in... Read More about Lack of detection of SARS-CoV-2 in wildlife from Kerala, India in 2020–21.

Lack of detection of SARS-CoV-2 in British wildlife 2020–21 and first description of a stoat (Mustela erminea) Minacovirus (2023)
Journal Article
Apaa, T., Withers, A. J., Mackenzie, L., Staley, C., Dessi, N., Blanchard, A., Bennett, M., Bremner-Harrison, S., Chadwick, E. A., Hailer, F., Harrison, S. W. R., Lambin, X., Loose, M., Mathews, F., & Tarlinton, R. (2023). Lack of detection of SARS-CoV-2 in British wildlife 2020–21 and first description of a stoat (Mustela erminea) Minacovirus. Journal of General Virology, 104(12), https://doi.org/10.1099/jgv.0.001917

Repeat spillover of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) into new hosts has highlighted the critical role of cross-species transmission of coronaviruses and establishment of new reservoirs of virus in pandemic and epizootic sp... Read More about Lack of detection of SARS-CoV-2 in British wildlife 2020–21 and first description of a stoat (Mustela erminea) Minacovirus.

COMPARISON BETWEEN SANGER, ILLUMINA AND NANOPORE SEQUENCING EVIDENCING INTRA-HOST VARIATION OF FELINE LEUKEMIA VIRUS THAT INFECTS DOMESTIC CATS. (2023)
Preprint / Working Paper
Castillo-Aliaga, C., Blanchard, A. M., Castro-Seriche, S., Hidalgo-Hermoso, E., Jerez-Morales, A., Loose, M. W., & Tarlinton, R. E. (2023). COMPARISON BETWEEN SANGER, ILLUMINA AND NANOPORE SEQUENCING EVIDENCING INTRA-HOST VARIATION OF FELINE LEUKEMIA VIRUS THAT INFECTS DOMESTIC CATS

Knowledge of Feline Leukemia Virus (FeLV) sequence variation has mainly been developed using Sanger sequencing methods. However, advances in next generation sequencing methods and their broad use in laboratories has been changing our understanding of... Read More about COMPARISON BETWEEN SANGER, ILLUMINA AND NANOPORE SEQUENCING EVIDENCING INTRA-HOST VARIATION OF FELINE LEUKEMIA VIRUS THAT INFECTS DOMESTIC CATS..

Lack of detection of SARS-CoV-2 in British wildlife 2020-21 and first description of a stoat (Mustela erminea) Minacovirus (2023)
Preprint / Working Paper
Apaa, T., Withers, A. J., McKenzie, L., Staley, C., Blanchard, A., Bennett, M., Bremner-Harrison, S., Chadwick, E. A., Hailer, F., Harrison, S. W., Lambin, X., Loose, M., Mathews, F., & Tarlinton, R. (2023). Lack of detection of SARS-CoV-2 in British wildlife 2020-21 and first description of a stoat (Mustela erminea) Minacovirus

Repeat spillover of SARS-CoV-2 into new hosts has highlighted the critical role of cross species transmission of coronaviruses and establishment of new reservoirs of virus in pandemic and epizootic spread of coronaviruses. Species particularly suscep... Read More about Lack of detection of SARS-CoV-2 in British wildlife 2020-21 and first description of a stoat (Mustela erminea) Minacovirus.

Lack of detection of SARS-CoV-2 in Wildlife from Kerala, India in 2020-21 (2023)
Preprint / Working Paper
Zachariah, A., Krishnankutty, S. P., Manazhi, J., Omanakuttan, V., Santosh, S., Blanchard, A. M., & Tarlinton, R. E. (2023). Lack of detection of SARS-CoV-2 in Wildlife from Kerala, India in 2020-21

Spill over of SARs-CoV-2 into a variety of wild and domestic animals has been an ongoing feature of the human pandemic. The establishment of a new reservoir in white tailed deer in North America and increasing divergence of the viruses circulating in... Read More about Lack of detection of SARS-CoV-2 in Wildlife from Kerala, India in 2020-21.

Advances in the understanding, detection and management of equine strangles (2023)
Journal Article
McLinden, L. A., Freeman, S. L., Daly, J., Blanchard, A., Kemp‐Symonds, J. G., & Waller, A. (2023). Advances in the understanding, detection and management of equine strangles. Equine Veterinary Education, 35(12), 662-672. https://doi.org/10.1111/eve.13845

Streptococcus equi subspecies equi (S. equi) is the causative organism of the upper respiratory disease of equids, strangles, characterised by pyrexia, lymphadenopathy and mucopurulent nasal discharge. Strangles was first reported over 750 years ago... Read More about Advances in the understanding, detection and management of equine strangles.

Sarbecoviruses of British horseshoe bats; sequence variation and epidemiology (2023)
Journal Article
Apaa, T., Withers, A. J., Staley, C., Blanchard, A., Bennett, M., Bremner-Harrison, S., Chadwick, E. A., Hailer, F., Harrison, S. W. R., Loose, M., Mathews, F., & Tarlinton, R. (2023). Sarbecoviruses of British horseshoe bats; sequence variation and epidemiology. Journal of General Virology, 104(6), https://doi.org/10.1099/jgv.0.001859

Horseshoe bats are the natural hosts of the Sarbecovirus subgenus that includes SARS-CoV and SARS-CoV- 2. Despite the devastating impact of the COVID-19 pandemic, there is still little known about the underlying epidemiology and virology of sarbecovi... Read More about Sarbecoviruses of British horseshoe bats; sequence variation and epidemiology.