Professor JAMES LEIGH's Outputs (17)

PIMMS-Dash: Accessible analysis, interrogation, and visualisation of high-throughput transposon insertion sequencing (TIS) data (2024)
Journal Article
Blanchard, A. M., Taylor, A., Warry, A., Shephard, F., Curwen, A., Leigh, J. A., Emes, R. D., & Egan, S. A. (2024). PIMMS-Dash: Accessible analysis, interrogation, and visualisation of high-throughput transposon insertion sequencing (TIS) data. Computational and Structural Biotechnology Journal, 23, 3780-3783. https://doi.org/10.1016/j.csbj.2024.10.025

Motivation: Current methods for visualising and interrogating high-throughput transposon insertion mutagenesis sequencing (TIS) data requires a significant time investment in learning bioinformatics, often producing static figures that do not facilit... Read More about PIMMS-Dash: Accessible analysis, interrogation, and visualisation of high-throughput transposon insertion sequencing (TIS) data.

Priming from within: TLR2 dependent but receptor independent activation of the mammary macrophage inflammasome by Streptococcus uberis (2024)
Journal Article
Hinds, A., Ward, P., Archer, N., & Leigh, J. (2024). Priming from within: TLR2 dependent but receptor independent activation of the mammary macrophage inflammasome by Streptococcus uberis. Frontiers in Cellular and Infection Microbiology, 14, Article 1444178. https://doi.org/10.3389/fcimb.2024.1444178

Introduction: Streptococcus uberis is a member of the pyogenic cluster of Streptococcus commonly associated with intramammary infection and mastitis in dairy cattle. It is a poorly controlled globally endemic pathogen responsible for a significant ca... Read More about Priming from within: TLR2 dependent but receptor independent activation of the mammary macrophage inflammasome by Streptococcus uberis.

A core genome multi-locus sequence typing scheme for Streptococcus uberis: an evolution in typing a genetically diverse pathogen (2024)
Journal Article
Whiley, D., Jolley, K., Blanchard, A., Coffey, T., & Leigh, J. (2024). A core genome multi-locus sequence typing scheme for Streptococcus uberis: an evolution in typing a genetically diverse pathogen. Microbial Genomics, 10(3), https://doi.org/10.1099/mgen.0.001225

Streptococcus uberis is a globally endemic and poorly controlled cause of bovine mastitis impacting the sustainability of the modern dairy industry. A core genome was derived from 579 newly sequenced S. uberis isolates, along with 305 publicly availa... Read More about A core genome multi-locus sequence typing scheme for Streptococcus uberis: an evolution in typing a genetically diverse pathogen.

Reproducible isolation of bovine mammary macrophages for analysis of host pathogen interactions (2024)
Journal Article
Tomes, A., Archer, N., & Leigh, J. (2024). Reproducible isolation of bovine mammary macrophages for analysis of host pathogen interactions. BMC Veterinary Research, 20, Article 96. https://doi.org/10.1186/s12917-024-03944-w

Background: Macrophages residing in milk are vital during intramammary infections. This study sought to develop a method enabling the investigation of macrophage responses to pathogens. Streptococcus uberis is the predominant cause of bovine mastitis... Read More about Reproducible isolation of bovine mammary macrophages for analysis of host pathogen interactions.

A paradox in bacterial pathogenesis: Activation of the local macrophage inflammasome is required for virulence of streptococcus uberis (2020)
Journal Article
Archer, N., Egan, S. A., Coffey, T. J., Emes, R. D., Addis, M. F., Ward, P. N., Blanchard, A. M., & Leigh, J. A. (2020). A paradox in bacterial pathogenesis: Activation of the local macrophage inflammasome is required for virulence of streptococcus uberis. Pathogens, 9(12), 1-23. https://doi.org/10.3390/pathogens9120997

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Streptococcus uberis is a common cause of intramammary infection and mastitis in dairy cattle. Unlike other mammary pathogens, S. uberis evades detection by mammary epithelial cells, and the h... Read More about A paradox in bacterial pathogenesis: Activation of the local macrophage inflammasome is required for virulence of streptococcus uberis.

Complete genome sequence of Streptococcus agalactiae strain 01173, isolated from Kuwaiti wild fish (2020)
Journal Article
Santi, M., Egan, S. A., Leigh, J. A., Verner-Jeffreys, D. W., & Blanchard, A. M. (2020). Complete genome sequence of Streptococcus agalactiae strain 01173, isolated from Kuwaiti wild fish. Microbiology Resource Announcements, 9(36), Article e00674-20. https://doi.org/10.1128/MRA.00674-20

© 2020 Santi et al. Here, we report the complete genome of piscine Streptococcus agalactiae 01173 serotype Ia, which was generated using long-read sequencing technology. The bacteria were isolated from wild fish displaying signs of streptococcosis, f... Read More about Complete genome sequence of Streptococcus agalactiae strain 01173, isolated from Kuwaiti wild fish.

Identification of genes required for the fitness of Streptococcus equi subsp. equi in whole equine blood and hydrogen peroxide (2020)
Journal Article
Charbonneau, A. R. L., Taylor, E., Mitchell, C. J., Robinson, C., Cain, A. K., Leigh, J. A., Maskell, D. J., & Waller, A. S. (2020). Identification of genes required for the fitness of Streptococcus equi subsp. equi in whole equine blood and hydrogen peroxide. Microbial Genomics, 6(4), https://doi.org/10.1099/mgen.0.000362

The availability of next-generation sequencing techniques provides an unprecedented opportunity for the assignment of gene function. Streptococcus equi subspecies equi is the causative agent of strangles in horses, one of the most prevalent and impor... Read More about Identification of genes required for the fitness of Streptococcus equi subsp. equi in whole equine blood and hydrogen peroxide.

Defining the ABC of gene essentiality in streptococci (2017)
Journal Article
Charbonneau, A. R. L., Forman, O. P., Cain, A. K., Newland, G., Robinson, C., Boursnell, M., Parkhill, J., Leigh, J. A., Maskell, D. J., & Waller, A. S. (2017). Defining the ABC of gene essentiality in streptococci. BMC Genomics, 18(1), Article 426. https://doi.org/10.1186/s12864-017-3794-3

Background: Utilising next generation sequencing to interrogate saturated bacterial mutant libraries provides unprecedented information for the assignment of genome-wide gene essentiality. Exposure of saturated mutant libraries to specific conditions... Read More about Defining the ABC of gene essentiality in streptococci.

The bio-physics of condensation of divalent cations into the bacterial wall has implications for growth of Gram-positive bacteria (2016)
Journal Article
Rauch, C., Cherkaoui-Rbati, M., Egan, S. A., & Leigh, J. A. (2017). The bio-physics of condensation of divalent cations into the bacterial wall has implications for growth of Gram-positive bacteria. BBA - Biomembranes, 1859(2), https://doi.org/10.1016/j.bbamem.2016.12.002

Background: The anionic-polyelectrolyte nature of the wall of Gram-positive bacteria has long been suspected to be involved in homeostasis of essential cations and bacterial growth. A better understanding of the coupling between the biophysics and th... Read More about The bio-physics of condensation of divalent cations into the bacterial wall has implications for growth of Gram-positive bacteria.

PIMMS (Pragmatic Insertional Mutation Mapping System) laboratory methodology a readily accessible tool for identification of essential genes in Streptococcus (2016)
Journal Article
Blanchard, A. M., Egan, S. A., Emes, R. D., Warry, A., & Leigh, J. A. (2016). PIMMS (Pragmatic Insertional Mutation Mapping System) laboratory methodology a readily accessible tool for identification of essential genes in Streptococcus. Frontiers in Microbiology, 7(1645), https://doi.org/10.3389/fmicb.2016.01645

The Pragmatic Insertional Mutation Mapping (PIMMS) laboratory protocol was developed alongside various bioinformatics packages (Blanchard et al., 2015) to enable detection of essential and conditionally essential genes in Streptococcus and related ba... Read More about PIMMS (Pragmatic Insertional Mutation Mapping System) laboratory methodology a readily accessible tool for identification of essential genes in Streptococcus.

Streptococcus uberis strains isolated from the bovine mammary gland evade immune recognition by mammary epithelial cells, but not of macrophages (2016)
Journal Article
Günther, J., Czabanska, A., Bauer, I., Leigh, J. A., Holst, O., & Seyfert, H.-M. (2016). Streptococcus uberis strains isolated from the bovine mammary gland evade immune recognition by mammary epithelial cells, but not of macrophages. Veterinary Research, 47(13), https://doi.org/10.1186/s13567-015-0287-8

Streptococcus uberis is frequently isolated from the mammary gland of dairy cattle. Infection with some strains can induce mild subclinical inflammation whilst others induce severe inflammation and clinical mastitis. We compared here the inflammatory... Read More about Streptococcus uberis strains isolated from the bovine mammary gland evade immune recognition by mammary epithelial cells, but not of macrophages.

Molecular epidemiology of Streptococcus uberis clinical mastitis in dairy herds: strain heterogeneity and transmission (2015)
Journal Article
Davies, P., Archer, S. C., Emes, R. D., Bradley, A. J., Leigh, J., & Green, M. J. (2016). Molecular epidemiology of Streptococcus uberis clinical mastitis in dairy herds: strain heterogeneity and transmission. Journal of Clinical Microbiology, 54(1), https://doi.org/10.1128/JCM.01583-15

Multi Locus Sequence Typing was successfully completed on 494 isolates of S. uberis from clinical mastitis cases in a study of 52 commercial dairy herds over a 12 month period. In total, 195 sequence types (STs) were identified. S. uberis mastitis ca... Read More about Molecular epidemiology of Streptococcus uberis clinical mastitis in dairy herds: strain heterogeneity and transmission.

Virulence related sequences; insights provided by comparative genomics of Streptococcus uberis of differing virulence (2015)
Journal Article
Hossain, M., Emes, R., Coffey, T., Egan, S., Ward, P. N., Wilson, R., & Leigh, J. A. (2015). Virulence related sequences; insights provided by comparative genomics of Streptococcus uberis of differing virulence. BMC Genomics, 16, 1-13. https://doi.org/10.1186/s12864-015-1512-6

Background

Streptococcus uberis, a Gram-positive, catalase-negative member of the family Streptococcaceae is an important environmental pathogen responsible for a significant proportion of subclinical and clinical bovine intramammary infections. C... Read More about Virulence related sequences; insights provided by comparative genomics of Streptococcus uberis of differing virulence.

Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System (2015)
Journal Article
Blanchard, A. M., Leigh, J. A., Egan, S. A., & Emes, R. D. (2015). Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System. Frontiers in Genetics, 6, Article e139. https://doi.org/10.3389/fgene.2015.00139

The PIMMS (Pragmatic Insertional Mutation Mapping System) pipeline has beendeveloped for simple conditionally essential genome discovery experiments in bacteria.Capable of using raw sequence data files alongside a FASTA sequence of thereference genom... Read More about Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System.

Theoretical evaluation of wall teichoic acids in the cavitation-mediated pores formation in Gram-positive bacteria subjected to an electric field (2014)
Journal Article
Rauch, C., & Leigh, J. A. (in press). Theoretical evaluation of wall teichoic acids in the cavitation-mediated pores formation in Gram-positive bacteria subjected to an electric field. BBA - General Subjects, 1850(4), https://doi.org/10.1016/j.bbagen.2014.12.004

Background: Electroporation is a method of choice to transform living cells. The ability of electroporation to transfer small or large chemicals across the lipid bilayer membrane of eukaryotic cells or Gram-negative bacteria relies on the formation o... Read More about Theoretical evaluation of wall teichoic acids in the cavitation-mediated pores formation in Gram-positive bacteria subjected to an electric field.

Two distinct populations of Bovine IL-17+ T-cells can be induced and WC1+IL-17+γδ T-cells are effective killers of protozoan parasites (2014)
Journal Article
Peckham, R., Brill, R., Foster, D. S., Bowen, A., Leigh, J. A., Coffey, T. J., & Flynn, R. J. (2014). Two distinct populations of Bovine IL-17+ T-cells can be induced and WC1+IL-17+γδ T-cells are effective killers of protozoan parasites. Scientific Reports, 4, Article 5431. https://doi.org/10.1038/srep05431

IL-17 has emerged as a key player in the immune system, exhibiting roles in protection from infectious diseases and promoting inflammation in autoimmunity. Initially thought to be CD4 T-cell-derived, the sources of IL-17 are now known to be varied an... Read More about Two distinct populations of Bovine IL-17+ T-cells can be induced and WC1+IL-17+γδ T-cells are effective killers of protozoan parasites.

Sortase anchored proteins of Streptococcus uberis play major roles in the pathogenesis of bovine mastitis in dairy cattle (2010)
Journal Article
Leigh, J. A., Egan, S. A., Ward, P. N., Field, T. R., & Coffey, T. J. (2010). Sortase anchored proteins of Streptococcus uberis play major roles in the pathogenesis of bovine mastitis in dairy cattle. Veterinary Research, 41(5), Article 63. https://doi.org/10.1051/vetres/2010036

Streptococcus uberis, strain 0140J, contains a single copy sortase A (srtA), encoding a transamidase capable of covalently anchoring specific proteins to peptidoglycan. Unlike the wild-type, an isogenic mutant carrying an inactivating ISS1 insertion... Read More about Sortase anchored proteins of Streptococcus uberis play major roles in the pathogenesis of bovine mastitis in dairy cattle.