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Professor JAMES LEIGH's Outputs (13)

PIMMS-Dash: Accessible analysis, interrogation, and visualisation of high-throughput transposon insertion sequencing (TIS) data (2024)
Journal Article
Blanchard, A. M., Taylor, A., Warry, A., Shephard, F., Curwen, A., Leigh, J. A., Emes, R. D., & Egan, S. A. (2024). PIMMS-Dash: Accessible analysis, interrogation, and visualisation of high-throughput transposon insertion sequencing (TIS) data. Computational and Structural Biotechnology Journal, 23, 3780-3783. https://doi.org/10.1016/j.csbj.2024.10.025

Motivation: Current methods for visualising and interrogating high-throughput transposon insertion mutagenesis sequencing (TIS) data requires a significant time investment in learning bioinformatics, often producing static figures that do not facilit... Read More about PIMMS-Dash: Accessible analysis, interrogation, and visualisation of high-throughput transposon insertion sequencing (TIS) data.

Priming from within: TLR2 dependent but receptor independent activation of the mammary macrophage inflammasome by Streptococcus uberis (2024)
Journal Article
Hinds, A., Ward, P., Archer, N., & Leigh, J. (2024). Priming from within: TLR2 dependent but receptor independent activation of the mammary macrophage inflammasome by Streptococcus uberis. Frontiers in Cellular and Infection Microbiology, 14, Article 1444178. https://doi.org/10.3389/fcimb.2024.1444178

Introduction: Streptococcus uberis is a member of the pyogenic cluster of Streptococcus commonly associated with intramammary infection and mastitis in dairy cattle. It is a poorly controlled globally endemic pathogen responsible for a significant ca... Read More about Priming from within: TLR2 dependent but receptor independent activation of the mammary macrophage inflammasome by Streptococcus uberis.

A core genome multi-locus sequence typing scheme for Streptococcus uberis: an evolution in typing a genetically diverse pathogen (2024)
Journal Article
Whiley, D., Jolley, K., Blanchard, A., Coffey, T., & Leigh, J. (2024). A core genome multi-locus sequence typing scheme for Streptococcus uberis: an evolution in typing a genetically diverse pathogen. Microbial Genomics, 10(3), https://doi.org/10.1099/mgen.0.001225

Streptococcus uberis is a globally endemic and poorly controlled cause of bovine mastitis impacting the sustainability of the modern dairy industry. A core genome was derived from 579 newly sequenced S. uberis isolates, along with 305 publicly availa... Read More about A core genome multi-locus sequence typing scheme for Streptococcus uberis: an evolution in typing a genetically diverse pathogen.

Reproducible isolation of bovine mammary macrophages for analysis of host pathogen interactions (2024)
Journal Article
Tomes, A., Archer, N., & Leigh, J. (2024). Reproducible isolation of bovine mammary macrophages for analysis of host pathogen interactions. BMC Veterinary Research, 20, Article 96. https://doi.org/10.1186/s12917-024-03944-w

Background: Macrophages residing in milk are vital during intramammary infections. This study sought to develop a method enabling the investigation of macrophage responses to pathogens. Streptococcus uberis is the predominant cause of bovine mastitis... Read More about Reproducible isolation of bovine mammary macrophages for analysis of host pathogen interactions.

A paradox in bacterial pathogenesis: Activation of the local macrophage inflammasome is required for virulence of streptococcus uberis (2020)
Journal Article
Archer, N., Egan, S. A., Coffey, T. J., Emes, R. D., Addis, M. F., Ward, P. N., …Leigh, J. A. (2020). A paradox in bacterial pathogenesis: Activation of the local macrophage inflammasome is required for virulence of streptococcus uberis. Pathogens, 9(12), 1-23. https://doi.org/10.3390/pathogens9120997

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Streptococcus uberis is a common cause of intramammary infection and mastitis in dairy cattle. Unlike other mammary pathogens, S. uberis evades detection by mammary epithelial cells, and the h... Read More about A paradox in bacterial pathogenesis: Activation of the local macrophage inflammasome is required for virulence of streptococcus uberis.

Complete genome sequence of Streptococcus agalactiae strain 01173, isolated from Kuwaiti wild fish (2020)
Journal Article
Santi, M., Egan, S. A., Leigh, J. A., Verner-Jeffreys, D. W., & Blanchard, A. M. (2020). Complete genome sequence of Streptococcus agalactiae strain 01173, isolated from Kuwaiti wild fish. Microbiology Resource Announcements, 9(36), Article e00674-20. https://doi.org/10.1128/MRA.00674-20

© 2020 Santi et al. Here, we report the complete genome of piscine Streptococcus agalactiae 01173 serotype Ia, which was generated using long-read sequencing technology. The bacteria were isolated from wild fish displaying signs of streptococcosis, f... Read More about Complete genome sequence of Streptococcus agalactiae strain 01173, isolated from Kuwaiti wild fish.

Defining the ABC of gene essentiality in streptococci (2017)
Journal Article
Charbonneau, A. R. L., Forman, O. P., Cain, A. K., Newland, G., Robinson, C., Boursnell, M., …Waller, A. S. (2017). Defining the ABC of gene essentiality in streptococci. BMC Genomics, 18(1), Article 426. https://doi.org/10.1186/s12864-017-3794-3

Background: Utilising next generation sequencing to interrogate saturated bacterial mutant libraries provides unprecedented information for the assignment of genome-wide gene essentiality. Exposure of saturated mutant libraries to specific conditions... Read More about Defining the ABC of gene essentiality in streptococci.

Streptococcus uberis strains isolated from the bovine mammary gland evade immune recognition by mammary epithelial cells, but not of macrophages (2016)
Journal Article
Günther, J., Czabanska, A., Bauer, I., Leigh, J. A., Holst, O., & Seyfert, H.-M. (2016). Streptococcus uberis strains isolated from the bovine mammary gland evade immune recognition by mammary epithelial cells, but not of macrophages. Veterinary Research, 47(13), https://doi.org/10.1186/s13567-015-0287-8

Streptococcus uberis is frequently isolated from the mammary gland of dairy cattle. Infection with some strains can induce mild subclinical inflammation whilst others induce severe inflammation and clinical mastitis. We compared here the inflammatory... Read More about Streptococcus uberis strains isolated from the bovine mammary gland evade immune recognition by mammary epithelial cells, but not of macrophages.

Virulence related sequences; insights provided by comparative genomics of Streptococcus uberis of differing virulence (2015)
Journal Article

Background

Streptococcus uberis, a Gram-positive, catalase-negative member of the family Streptococcaceae is an important environmental pathogen responsible for a significant proportion of subclinical and clinical bovine intramammary infections. C... Read More about Virulence related sequences; insights provided by comparative genomics of Streptococcus uberis of differing virulence.

Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System (2015)
Journal Article
Blanchard, A. M., Leigh, J. A., Egan, S. A., & Emes, R. D. (2015). Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System. Frontiers in Genetics, 6, Article e139. https://doi.org/10.3389/fgene.2015.00139

The PIMMS (Pragmatic Insertional Mutation Mapping System) pipeline has beendeveloped for simple conditionally essential genome discovery experiments in bacteria.Capable of using raw sequence data files alongside a FASTA sequence of thereference genom... Read More about Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System.

Theoretical evaluation of wall teichoic acids in the cavitation-mediated pores formation in Gram-positive bacteria subjected to an electric field (2014)
Journal Article
Rauch, C., & Leigh, J. A. (in press). Theoretical evaluation of wall teichoic acids in the cavitation-mediated pores formation in Gram-positive bacteria subjected to an electric field. BBA - General Subjects, 1850(4), https://doi.org/10.1016/j.bbagen.2014.12.004

Background: Electroporation is a method of choice to transform living cells. The ability of electroporation to transfer small or large chemicals across the lipid bilayer membrane of eukaryotic cells or Gram-negative bacteria relies on the formation o... Read More about Theoretical evaluation of wall teichoic acids in the cavitation-mediated pores formation in Gram-positive bacteria subjected to an electric field.

Sortase anchored proteins of Streptococcus uberis play major roles in the pathogenesis of bovine mastitis in dairy cattle (2010)
Journal Article
Leigh, J. A., Egan, S. A., Ward, P. N., Field, T. R., & Coffey, T. J. (2010). Sortase anchored proteins of Streptococcus uberis play major roles in the pathogenesis of bovine mastitis in dairy cattle. Veterinary Research, 41(5), Article 63. https://doi.org/10.1051/vetres/2010036

Streptococcus uberis, strain 0140J, contains a single copy sortase A (srtA), encoding a transamidase capable of covalently anchoring specific proteins to peptidoglycan. Unlike the wild-type, an isogenic mutant carrying an inactivating ISS1 insertion... Read More about Sortase anchored proteins of Streptococcus uberis play major roles in the pathogenesis of bovine mastitis in dairy cattle.