Research Repository

See what's under the surface

Sortase anchored proteins of Streptococcus uberis play major roles in the pathogenesis of bovine mastitis in dairy cattle

Leigh, James A.; Egan, Sharon A.; Ward, Philip N.; Field, Terence R.; Coffey, Tracey J.

Authors

James A. Leigh

Sharon A. Egan

Philip N. Ward

Terence R. Field

Tracey J. Coffey

Abstract

Streptococcus uberis, strain 0140J, contains a single copy sortase A (srtA), encoding a transamidase capable of covalently anchoring specific proteins to peptidoglycan. Unlike the wild-type, an isogenic mutant carrying an inactivating ISS1 insertion within srtA was only able to infect the bovine mammary gland in a transient fashion. For the first 24 h post challenge, the srtA mutant colonised at a similar rate and number to the wild type strain, but unlike the wild type did not subsequently colonise in higher numbers. Similar levels of host cell infiltration were detected in response to infection with both strains, but only in those mammary quarters infected with the wild type strain were clinical signs of disease evident. Mutants that failed to express individual sortase substrate proteins (sub0135, sub0145, sub0207, sub0241, sub0826, sub0888, sub1095, sub1154, sub1370, and sub1730) were isolated and their virulence determined in the same challenge model. This revealed that mutants lacking sub0145, sub1095 and sub1154 were attenuated in cattle. These data demonstrate that a number of sortase anchored proteins each play a distinct, non-redundant and important role in pathogenesis of S. uberis infection within the lactating bovine mammary gland.

Journal Article Type Article
Publication Date Sep 1, 2010
Journal Veterinary Research
Print ISSN 0928-4249
Electronic ISSN 1297-9716
Publisher Springer Verlag
Peer Reviewed Peer Reviewed
Volume 41
Issue 5
Article Number 63
Institution Citation Leigh, J. A., Egan, S. A., Ward, P. N., Field, T. R., & Coffey, T. J. (2010). Sortase anchored proteins of Streptococcus uberis play major roles in the pathogenesis of bovine mastitis in dairy cattle. Veterinary Research, 41(5), doi:10.1051/vetres/2010036
DOI https://doi.org/10.1051/vetres/2010036
Keywords mastitis, S. uberis, virulence, sortase, vaccine
Publisher URL http://www.vetres.org/articles/vetres/abs/2010/05/v100007/v100007.html
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc/4.0

Files

srt challenge paper.pdf (602 Kb)
PDF

Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc/4.0




Downloadable Citations