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Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System

Blanchard, Adam M.; Leigh, James A.; Egan, Sharon A.; Emes, Richard D.

Authors

RICHARD EMES richard.emes@nottingham.ac.uk
Professor of Bioinformatics



Abstract

The PIMMS (Pragmatic Insertional Mutation Mapping System) pipeline has beendeveloped for simple conditionally essential genome discovery experiments in bacteria.Capable of using raw sequence data files alongside a FASTA sequence of thereference genome and GFF file, PIMMS will generate a tabulated output of each codingsequence with corresponding mapped insertions accompanied with normalized resultsenabling streamlined analysis. This allows for a quick assay of the genome to identifyconditionally essential genes on a standar d desktop computer prioritizing results forfurther investigation.

Journal Article Type Article
Publication Date Apr 9, 2015
Journal Frontiers in Genetics
Electronic ISSN 1664-8021
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 6
Article Number e139
APA6 Citation Blanchard, A. M., Leigh, J. A., Egan, S. A., & Emes, R. D. (2015). Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System. Frontiers in Genetics, 6, https://doi.org/10.3389/fgene.2015.00139
DOI https://doi.org/10.3389/fgene.2015.00139
Publisher URL http://journal.frontiersin.org/article/10.3389/fgene.2015.00139/abstract
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0
Additional Information This document is protected by copyright and was first published by Frontiers. All rights reserved. It is reproduced with permission.

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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