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Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System

Blanchard, Adam M.; Leigh, James A.; Egan, Sharon A.; Emes, Richard D.

Authors

RICHARD EMES richard.emes@nottingham.ac.uk
Professor of Bioinformatics



Abstract

The PIMMS (Pragmatic Insertional Mutation Mapping System) pipeline has beendeveloped for simple conditionally essential genome discovery experiments in bacteria.Capable of using raw sequence data files alongside a FASTA sequence of thereference genome and GFF file, PIMMS will generate a tabulated output of each codingsequence with corresponding mapped insertions accompanied with normalized resultsenabling streamlined analysis. This allows for a quick assay of the genome to identifyconditionally essential genes on a standar d desktop computer prioritizing results forfurther investigation.

Citation

Blanchard, A. M., Leigh, J. A., Egan, S. A., & Emes, R. D. (2015). Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System. Frontiers in Genetics, 6, https://doi.org/10.3389/fgene.2015.00139

Journal Article Type Article
Acceptance Date Mar 23, 2015
Online Publication Date Apr 9, 2015
Publication Date Apr 9, 2015
Deposit Date Dec 18, 2015
Publicly Available Date Dec 18, 2015
Journal Frontiers in Genetics
Electronic ISSN 1664-8021
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 6
Article Number e139
DOI https://doi.org/10.3389/fgene.2015.00139
Public URL http://eprints.nottingham.ac.uk/id/eprint/31135
Publisher URL http://journal.frontiersin.org/article/10.3389/fgene.2015.00139/abstract
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0
Additional Information This document is protected by copyright and was first published by Frontiers. All rights reserved. It is reproduced with permission.

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