Identification of genes required for the fitness of Streptococcus equi subsp. equi in whole equine blood and hydrogen peroxide

Charbonneau, Amelia R. L.; Taylor, Emma; Mitchell, Catriona J.; Robinson, Carl; Cain, Amy K.; Leigh, James A.; Maskell, Duncan J.; Waller, Andrew S.

Abstract

The availability of next-generation sequencing techniques provides an unprecedented opportunity for the assignment of gene function. Streptococcus equi subspecies equi is the causative agent of strangles in horses, one of the most prevalent and important diseases of equids worldwide. However, the live attenuated vaccines that are utilized to control this disease cause adverse reactions in some animals. Here, we employ transposon-directed insertion-site sequencing (TraDIS) to identify genes that are required for the fitness of S. equi in whole equine blood or in the presence of H2O2 to model selective pressures exerted by the equine immune response during infection. We report the fitness values of 1503 and 1471 genes, representing 94.5 and 92.5 % of non-essential genes in S. equi, following incubation in whole blood and in the presence of H2O2, respectively. Of these genes, 36 and 15 were identified as being important to the fitness of S. equi in whole blood or H2O2, respectively, with 14 genes being important in both conditions. Allelic replacement mutants were generated to validate the fitness results. Our data identify genes that are important for S. equi to resist aspects of the immune response in vitro, which can be exploited for the development of safer live attenuated vaccines to prevent strangles.

Citation

Charbonneau, A. R. L., Taylor, E., Mitchell, C. J., Robinson, C., Cain, A. K., Leigh, J. A., Maskell, D. J., & Waller, A. S. (2020). Identification of genes required for the fitness of Streptococcus equi subsp. equi in whole equine blood and hydrogen peroxide. Microbial Genomics, 6(4), https://doi.org/10.1099/mgen.0.000362

• Journal Article Type: Article
• Acceptance Date: Mar 12, 2020
• Online Publication Date: Mar 31, 2020
• Publication Date: Mar 31, 2020
• Deposit Date: Apr 2, 2020
• Publicly Available Date: Apr 2, 2020
• Journal: Microbial Genomics
• Electronic ISSN: 2057-5858
• Publisher: Microbiology Society
• Peer Reviewed: Peer Reviewed
• Volume: 6
• Issue: 4
• DOI: https://doi.org/10.1099/mgen.0.000362
• Public URL: https://nottingham-repository.worktribe.com/output/4242741
• Publisher URL: https://www.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.000362