All Outputs (20)

Development, characterization, and evaluation of a simple polymicrobial colony biofilm model for testing of antimicrobial wound dressings (2024)
Journal Article
Robertson, S. N., Romero, M., Fenn, S., Riedi, P. L. K., & Cámara, M. (2024). Development, characterization, and evaluation of a simple polymicrobial colony biofilm model for testing of antimicrobial wound dressings. Journal of Applied Microbiology, 135(3), Article lxae042. https://doi.org/10.1093/jambio/lxae042

Chronic wound infections are generally of polymicrobial nature with aerobic and anaerobic bacteria, as well as fungi frequently observed in them. Wound treatment involves a series of steps, including debridement of the wound, flushing, and often the... Read More about Development, characterization, and evaluation of a simple polymicrobial colony biofilm model for testing of antimicrobial wound dressings.

Ciprofloxacin poly(β-amino ester) conjugates enhance antibiofilm activity and slow the development of resistance (2024)
Journal Article
Kasza, K., Richards, B., Jones, S., Romero, M., Robertson, S. N., Hardie, K. R., …Alexander, C. (2024). Ciprofloxacin poly(β-amino ester) conjugates enhance antibiofilm activity and slow the development of resistance. ACS Applied Materials and Interfaces, 16(5), 5412-5425. https://doi.org/10.1021/acsami.3c14357

To tackle the emerging antibiotic resistance crisis, novel antimicrobial approaches are urgently needed. Bacterial biofilms are a particular concern in this context as they are responsible for over 80% of bacterial infections and are inherently more... Read More about Ciprofloxacin poly(β-amino ester) conjugates enhance antibiofilm activity and slow the development of resistance.

Design, Synthesis, and Evaluation of New 1H-Benzo[d]imidazole Based PqsR Inhibitors as Adjuvant Therapy for Pseudomonas aeruginosa Infections (2024)
Journal Article
Soukarieh, F., Mashabi, A., Richardson, W., Oton, E. V., Romero, M., Dubern, J., …Cámara, M. (2024). Design, Synthesis, and Evaluation of New 1H-Benzo[d]imidazole Based PqsR Inhibitors as Adjuvant Therapy for Pseudomonas aeruginosa Infections. Journal of Medicinal Chemistry, 67(2), 1008-1023. https://doi.org/10.1021/acs.jmedchem.3c00973

Pseudomonas aeruginosa is one of the top priority pathogens that requires immediate attention according to the World Health Organisation (WHO). Due to the alarming shortage of novel antimicrobials, targeting quorum sensing (QS), a bacterial cell to c... Read More about Design, Synthesis, and Evaluation of New 1H-Benzo[d]imidazole Based PqsR Inhibitors as Adjuvant Therapy for Pseudomonas aeruginosa Infections.

Probing Interkingdom Signaling Molecules via Liquid Extraction Surface Analysis-Mass Spectrometry (2023)
Journal Article
Robertson, S. N., Soukarieh, F., White, T. M., Camara, M., Romero, M., & Griffiths, R. L. (2023). Probing Interkingdom Signaling Molecules via Liquid Extraction Surface Analysis-Mass Spectrometry. Analytical Chemistry, 95(11), 5079-5086. https://doi.org/10.1021/acs.analchem.2c05703

Previously, metabolites diffused or secreted from microbial samples have been analyzed via liquid chromatography–mass spectrometry (LC–MS) approaches following lengthy extraction protocols. Here, we present a model system for growing biofilms on disc... Read More about Probing Interkingdom Signaling Molecules via Liquid Extraction Surface Analysis-Mass Spectrometry.

Translational challenges and opportunities in biofilm science: a BRIEF for the future (2022)
Journal Article
Highmore, C. J., Melaugh, G., Morris, R. J., Parker, J., Direito, S. O. L., Romero, M., …Bamford, N. C. (2022). Translational challenges and opportunities in biofilm science: a BRIEF for the future. npj Biofilms and Microbiomes, 8, Article 68. https://doi.org/10.1038/s41522-022-00327-7

Biofilms are increasingly recognised as a critical global issue in a multitude of industries impacting health, food and water security, marine sector, and industrial processes resulting in estimated economic cost of $5 trillion USD annually. A major... Read More about Translational challenges and opportunities in biofilm science: a BRIEF for the future.

ToxR is a c-di-GMP binding protein that modulates surface-associated behaviour in Pseudomonas aeruginosa (2022)
Journal Article
Camara, M., Dubern, J., Romero, M., Mai-Prochnow, A., Messina, M., Trampari, E., …Heeb, S. (2022). ToxR is a c-di-GMP binding protein that modulates surface-associated behaviour in Pseudomonas aeruginosa. npj Biofilms and Microbiomes, 8, Article 64. https://doi.org/10.1038/s41522-022-00325-9

Pseudomonas aeruginosa uses multiple protein regulators that work in tandem to control the production of a wide range of virulence factors and facilitate rapid adaptation to diverse environmental conditions. In this opportunistic pathogen, ToxR was k... Read More about ToxR is a c-di-GMP binding protein that modulates surface-associated behaviour in Pseudomonas aeruginosa.

Fluid dynamics and cell-bound Psl polysaccharide allows microplastic capture, aggregation and subsequent sedimentation by Pseudomonas aeruginosa in water (2022)
Journal Article
Romero, M., Carabelli, A., Swift, M., & Smith, M. (2022). Fluid dynamics and cell-bound Psl polysaccharide allows microplastic capture, aggregation and subsequent sedimentation by Pseudomonas aeruginosa in water. Environmental Microbiology, 24(3), 1560-1572. https://doi.org/10.1111/1462-2920.15916

Decades after incorporating plastics into consumer markets, research shows that these polymers have spread worldwide. Fragmentation of large debris leads to smaller particles, collectively called microplastics (MPs), which have become ubiquitous in a... Read More about Fluid dynamics and cell-bound Psl polysaccharide allows microplastic capture, aggregation and subsequent sedimentation by Pseudomonas aeruginosa in water.

Design and Evaluation of New Quinazolin-4(3 H)-one Derived PqsR Antagonists as Quorum Sensing Quenchers in Pseudomonas aeruginosa (2021)
Journal Article
Soukarieh, F., Mashabi, A., Richardson, W., Oton, E. V., Romero, M., Roberston, S. N., …Cámara, M. (2021). Design and Evaluation of New Quinazolin-4(3 H)-one Derived PqsR Antagonists as Quorum Sensing Quenchers in Pseudomonas aeruginosa. ACS Infectious Diseases, 7(9), 2666-2685. https://doi.org/10.1021/acsinfecdis.1c00175

P. aeruginosa (PA) continues to pose a threat to global public health due to its high levels of antimicrobial resistance (AMR). The ongoing AMR crisis has led to an alarming shortage of effective treatments for resistant microbes, and hence there is... Read More about Design and Evaluation of New Quinazolin-4(3 H)-one Derived PqsR Antagonists as Quorum Sensing Quenchers in Pseudomonas aeruginosa.

Genome-Wide Analysis of Targets for Post-Transcriptional Regulation by Rsm Proteins in Pseudomonas putida (2021)
Journal Article
Huertas-Rosales, Ó., Romero, M., Chan, K. G., Hong, K., Camara, M., Heeb, S., …Espinosa-Urgel, M. (2021). Genome-Wide Analysis of Targets for Post-Transcriptional Regulation by Rsm Proteins in Pseudomonas putida. Frontiers in Molecular Biosciences, 8, Article 624061. https://doi.org/10.3389/fmolb.2021.624061

© Copyright © 2021 Huertas-Rosales, Romero, Chan, Hong, Cámara, Heeb, Barrientos-Moreno, Molina-Henares, Travieso, Ramos-González and Espinosa-Urgel. Post-transcriptional regulation is an important step in the control of bacterial gene expression in... Read More about Genome-Wide Analysis of Targets for Post-Transcriptional Regulation by Rsm Proteins in Pseudomonas putida.

AhaP, A Quorum Quenching Acylase from Psychrobacter sp. M9-54-1 That Attenuates Pseudomonas aeruginosa and Vibrio coralliilyticus Virulence (2021)
Journal Article
Reina, J. C., Romero, M., Salto, R., Cámara, M., & Llamas, I. (2021). AhaP, A Quorum Quenching Acylase from Psychrobacter sp. M9-54-1 That Attenuates Pseudomonas aeruginosa and Vibrio coralliilyticus Virulence. Marine Drugs, 19(1), Article 16. https://doi.org/10.3390/md19010016

Although Psychrobacter strain M9-54-1 had been previously isolated from the microbiota of holothurians and shown to degrade quorum sensing (QS) signal molecules C6 and C10-homoserine lactone (HSL), little was known about the gene responsible for this... Read More about AhaP, A Quorum Quenching Acylase from Psychrobacter sp. M9-54-1 That Attenuates Pseudomonas aeruginosa and Vibrio coralliilyticus Virulence.

Achieving Microparticles with Cell-Instructive Surface Chemistry by Using Tunable Co-Polymer Surfactants (2020)
Journal Article
Dundas, A. A., Cuzzucoli Crucitti, V., Haas, S., Dubern, J., Latif, A., Romero, M., …Irvine, D. J. (2020). Achieving Microparticles with Cell-Instructive Surface Chemistry by Using Tunable Co-Polymer Surfactants. Advanced Functional Materials, 30(36), https://doi.org/10.1002/adfm.202001821

© 2020 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim A flow-focusing microfluidic device is used to produce functionalized monodisperse polymer particles with surface chemistries designed to control bacterial biofilm formatio... Read More about Achieving Microparticles with Cell-Instructive Surface Chemistry by Using Tunable Co-Polymer Surfactants.

Hit Identification of New Potent PqsR Antagonists as Inhibitors of Quorum Sensing in Planktonic and Biofilm Grown Pseudomonas aeruginosa (2020)
Journal Article
Soukarieh, F., Liu, R., Romero, M., Roberston, S. N., Richardson, W., Lucanto, S., …Stocks, M. J. (2020). Hit Identification of New Potent PqsR Antagonists as Inhibitors of Quorum Sensing in Planktonic and Biofilm Grown Pseudomonas aeruginosa. Frontiers in Chemistry, 8, Article 204. https://doi.org/10.3389/fchem.2020.00204

© Copyright © 2020 Soukarieh, Liu, Romero, Roberston, Richardson, Lucanto, Oton, Qudus, Mashabi, Grossman, Ali, Sou, Kukavica-Ibrulj, Levesque, Bergström, Halliday, Mistry, Emsley, Heeb, Williams, Cámara and Stocks. Current treatments for Pseudomonas... Read More about Hit Identification of New Potent PqsR Antagonists as Inhibitors of Quorum Sensing in Planktonic and Biofilm Grown Pseudomonas aeruginosa.

Dual Bioresponsive Antibiotic and Quorum Sensing Inhibitor Combination Nanoparticles for Treatment of Pseudomonas aeruginosa Biofilms In Vitro and Ex Vivo (2019)
Journal Article
Singh, N., Romero, M., Travanut, A., Monteiro, P., Jordana-Lluch, E., Hardie, K. R., …Alexander, C. (2019). Dual Bioresponsive Antibiotic and Quorum Sensing Inhibitor Combination Nanoparticles for Treatment of Pseudomonas aeruginosa Biofilms In Vitro and Ex Vivo. Biomaterials Science, 10(7), 4099-4111. https://doi.org/10.1039/C9BM00773C

Many debilitating infections result from persistent microbial biofilms that do not respond to conventional antibiotic regimens. A potential method to treat such chronic infections is to combine agents which interfere with bacterial biofilm developmen... Read More about Dual Bioresponsive Antibiotic and Quorum Sensing Inhibitor Combination Nanoparticles for Treatment of Pseudomonas aeruginosa Biofilms In Vitro and Ex Vivo.

Biocompatible unimolecular micelles obtained via the Passerini reaction as versatile nanocarriers for potential medical applications (2018)
Journal Article
Oelmann, S., Travanut, A., Barther, D., Romero, M., Howdle, S. M., Alexander, C., & Meier, M. A. (2019). Biocompatible unimolecular micelles obtained via the Passerini reaction as versatile nanocarriers for potential medical applications. Biomacromolecules, 20(1), 90-101. https://doi.org/10.1021/acs.biomac.8b00592

A Passerini three-component polymerization was performed for the synthesis of amphiphilic star-shaped block copolymers with hydrophobic cores and hydrophilic coronae. The degree of polymerization of the hydrophobic core was varied from 5 to 10 repeat... Read More about Biocompatible unimolecular micelles obtained via the Passerini reaction as versatile nanocarriers for potential medical applications.

Effect of surfactant on Pseudomonas aeruginosa colonization of polymer microparticles and flat films (2018)
Journal Article
Hüsler, A., Haas, S., Parry, L., Romero, M., Nisisako, T., Williams, P., …Alexander, M. R. (2018). Effect of surfactant on Pseudomonas aeruginosa colonization of polymer microparticles and flat films. RSC Advances, 8(28), https://doi.org/10.1039/c8ra01491d

Micro- and nanoparticles are of great interest because of their potential for trafficking into the body for applications such as low-fouling coatings on medical devices, drug delivery in pharmaceutics and cell carriers in regenerative medicine strate... Read More about Effect of surfactant on Pseudomonas aeruginosa colonization of polymer microparticles and flat films.

2-Tridecanone impacts surface-associated bacterial behaviours and hinders plant-bacteria interactions (2018)
Journal Article
López-Lara, I. M., Nogales, J., Pech-Canul, A., Calatrava-Morales, N., Bernabéu-Roda, L. M., Durán, P., …Soto, M. J. (2018). 2-Tridecanone impacts surface-associated bacterial behaviours and hinders plant-bacteria interactions. Environmental Microbiology, 20(6), 2049-2065. https://doi.org/10.1111/1462-2920.14083

Surface motility and biofilm formation are behaviours which enable bacteria to infect their hosts and are controlled by different chemical signals. In the plant symbiotic alpha‐proteobacterium Sinorhizobium meliloti, the lack of long‐chain fatty acyl... Read More about 2-Tridecanone impacts surface-associated bacterial behaviours and hinders plant-bacteria interactions.

Genome-wide mapping of the RNA targets of the Pseudomonas aeruginosa riboregulatory protein RsmN (2018)
Journal Article
Romero, M., Silistre, H., Lovelock, L., Wright, V. J., Kok, G., Kar-Wai, H., …Heeb, S. (2018). Genome-wide mapping of the RNA targets of the Pseudomonas aeruginosa riboregulatory protein RsmN. Nucleic Acids Research, 46(13), 6823-6840. https://doi.org/10.1093/nar/gky324

Pseudomonads typically carry multiple non-identical alleles of the post-transcriptional regulator rsmA. In P. aeruginosa, RsmN is notable in that its structural rearrangement confers distinct and overlapping functions with RsmA. However, little is k... Read More about Genome-wide mapping of the RNA targets of the Pseudomonas aeruginosa riboregulatory protein RsmN.

The Pseudomonas putida CsrA/RsmA homologues negatively affect c-di-GMP pools and biofilm formation through the GGDEF/EAL response regulator CfcR (2017)
Journal Article
Huertas-Rosales, Ó., Romero, M., Heeb, S., Espinosa-Urgel, M., Cámara, M., & Ramos-González, M. I. (in press). The Pseudomonas putida CsrA/RsmA homologues negatively affect c-di-GMP pools and biofilm formation through the GGDEF/EAL response regulator CfcR. Environmental Microbiology, 19(9), https://doi.org/10.1111/1462-2920.13848

Expression of cfcR, encoding the only GGDEF/EAL response regulator in Pseudomonas putida, is transcriptionally regulated by RpoS, ANR and FleQ, and the functionality of CfcR as a diguanylate cyclase requires the multisensor CHASE3/GAF hybrid histidin... Read More about The Pseudomonas putida CsrA/RsmA homologues negatively affect c-di-GMP pools and biofilm formation through the GGDEF/EAL response regulator CfcR.

RsmW, Pseudomonas aeruginosa small non-coding RsmA-binding RNA upregulated in biofilm versus planktonic growth conditions (2016)
Journal Article
Miller, C. L., Romero, M., Karna, S. R., Chen, T., Heeb, S., & Leung, K. P. (2016). RsmW, Pseudomonas aeruginosa small non-coding RsmA-binding RNA upregulated in biofilm versus planktonic growth conditions. BMC Microbiology, 16, Article 155. https://doi.org/10.1186/s12866-016-0771-y

BACKGROUND: Biofilm development, specifically the fundamentally adaptive switch from acute to chronic infection phenotypes, requires global regulators and small non-coding regulatory RNAs (sRNAs). This work utilized RNA-sequencing (RNA-seq) to detect... Read More about RsmW, Pseudomonas aeruginosa small non-coding RsmA-binding RNA upregulated in biofilm versus planktonic growth conditions.

Thermal behaviour of blind-bolted connections to hollow and concrete-filled steel tubular columns (2015)
Journal Article
Pascual, A. M., Romero, M., & Tizani, W. (2015). Thermal behaviour of blind-bolted connections to hollow and concrete-filled steel tubular columns. Journal of Constructional Steel Research, 107, https://doi.org/10.1016/j.jcsr.2015.01.014

This paper reports on the thermal analysis of blind-bolts connected to concrete filled steel tube (CFST) and hollow steel section (HSS) columns. The aim is therefore the investigation of the temperature distribution in the connected sections and the... Read More about Thermal behaviour of blind-bolted connections to hollow and concrete-filled steel tubular columns.