All Outputs (219)

Transplantation of bone marrow derived macrophages reduces markers of neuropathology in an APP/PS1 mouse model (2019)
Journal Article
Costa-Marques, L., Arnold, K., Pardon, M. C., Leovsky, C., Swarbrick, S., Fabian, C., & Stolzing, A. (2019). Transplantation of bone marrow derived macrophages reduces markers of neuropathology in an APP/PS1 mouse model. Translational Neurodegeneration, 8, Article 33. https://doi.org/10.1186/s40035-019-0173-9

© 2019 The Author(s). Background: We investigated early hallmarks of putative therapeutic effects following systemic transplantation of bone marrow derived macrophages (BM-M) in APP/PS1 transgenic mice. Method: BM-M were transplanted into the tail ve... Read More about Transplantation of bone marrow derived macrophages reduces markers of neuropathology in an APP/PS1 mouse model.

Tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor complex (2019)
Preprint / Working Paper
Koo, C. Z., Harrison, N., Noy, P. J., Szyroka, J., Matthews, A. L., Hsia, H.-E., Mueller, S. A., Tüshaus, J., Goulding, J., Willis, K., Apicella, C., Cragoe, B., Davis, E., Keles, M., Malinova, A., McFarlane, T. A., Morrison, P. R., Sykes, M. C., Ahmed, H., Di Maio, A., …Tomlinson, M. G. (2019). Tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor complex

A disintegrin and metalloprotease 10 (ADAM10) is essential for embryonic development and impacts on diseases such as cancer, Alzheimer’s and inflammatory diseases. ADAM10 is a ‘molecular scissor’ that proteolytically cleaves the extracellular region... Read More about Tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor complex.

Does video feedback & peer observation offer a valid method of reinforcing oral presentation training for undergraduate biochemists? (2019)
Journal Article
Simpson, T., Holden, K., Merrick, D., Dawson, S., & Bedford, L. (2019). Does video feedback & peer observation offer a valid method of reinforcing oral presentation training for undergraduate biochemists?. Higher Education Pedagogies, 4(1), 262-283. https://doi.org/10.1080/23752696.2019.1587717

Presentations assessing public speaking skills are common features of undergraduate curricula. Performance feedback has often been traditionally limited to staff, yet students acting as peer assessors can also be a useful feedback source. Additionall... Read More about Does video feedback & peer observation offer a valid method of reinforcing oral presentation training for undergraduate biochemists?.

Nitric oxide regulates the firing rate of neuronal subtypes in the guinea pig ventral cochlear nucleus (2019)
Journal Article
Hockley, A., Berger, J. I., Smith, P. A., Palmer, A. R., & Wallace, M. N. (2020). Nitric oxide regulates the firing rate of neuronal subtypes in the guinea pig ventral cochlear nucleus. European Journal of Neuroscience, 51(4), 963-983. https://doi.org/10.1111/ejn.14572

The gaseous free radical, nitric oxide (NO) acts as a ubiquitous neuromodulator, contributing to synaptic plasticity in a complex way that can involve either long term potentiation or depression. It is produced by neuronal nitric oxide synthase (nNOS... Read More about Nitric oxide regulates the firing rate of neuronal subtypes in the guinea pig ventral cochlear nucleus.

Inhibition of Piezo1 attenuates demyelination in the central nervous system (2019)
Journal Article
Velasco‐Estevez, M., Gadalla, K. K. E., Liñan‐Barba, N., Cobb, S., Dev, K. K., & Sheridan, G. K. (2020). Inhibition of Piezo1 attenuates demyelination in the central nervous system. Glia, 68(2), 356-375. https://doi.org/10.1002/glia.23722

Piezo1 is a mechanosensitive ion channel that facilitates the translation of extracellular mechanical cues to intracellular molecular signaling cascades through a process termed, mechanotransduction. In the central nervous system (CNS), mechanically... Read More about Inhibition of Piezo1 attenuates demyelination in the central nervous system.

A composite Gelatin/hyaluronic acid hydrogel as an ECM mimic for developing mesenchymal stem cell derived epithelial tissue patches (2019)
Journal Article
Kumar, P., Ciftci, S., Barthes, J., Knopf-Marques, H., Muller, C. B., Debry, C., Vrana, N. E., & Ghaemmaghami, A. M. (2020). A composite Gelatin/hyaluronic acid hydrogel as an ECM mimic for developing mesenchymal stem cell derived epithelial tissue patches. Journal of Tissue Engineering and Regenerative Medicine, 14(1), 45-57. https://doi.org/10.1002/term.2962

Here we report fabrication of Gelatin based biocomposite films and their application in developing epithelial patches. The films were loaded with an epithelial cell growth factor cocktail and used as an extracellular matrix mimic (ECM mimic) for in v... Read More about A composite Gelatin/hyaluronic acid hydrogel as an ECM mimic for developing mesenchymal stem cell derived epithelial tissue patches.

The effect of two selective A1-receptor agonists and the bitopic ligand VCP746 on heart rate and regional vascular conductance in conscious rats (2019)
Journal Article
Cooper, S. L., March, J., Sabbatini, A. R., Hill, S. J., Jörg, M., Scammells, P. J., & Woolard, J. (2020). The effect of two selective A1-receptor agonists and the bitopic ligand VCP746 on heart rate and regional vascular conductance in conscious rats. British Journal of Pharmacology, 177(2), 346-359. https://doi.org/10.1111/bph.14870

Background and purpose
Adenosine is a local mediator that regulates physiological and pathological processes via activation of four G protein‐coupled receptors (A1, A2A, A2B, A3). We have investigated the effect of two A1‐receptor selective agonists... Read More about The effect of two selective A1-receptor agonists and the bitopic ligand VCP746 on heart rate and regional vascular conductance in conscious rats.

A new set of endogenous control genes for use in quantitative real-time PCR experiments show that formin Ldia2dex transcripts are enriched in the early embryo of the pond snail Lymnaea stagnalis (Panpulmonata) (2019)
Journal Article
Johnson, H. F., & Davison, A. (2019). A new set of endogenous control genes for use in quantitative real-time PCR experiments show that formin Ldia2dex transcripts are enriched in the early embryo of the pond snail Lymnaea stagnalis (Panpulmonata). Journal of Molluscan Studies, 85(4), 388-396. https://doi.org/10.1093/mollus/eyz027

© 2019 The Author(s). Published by Oxford University Press on behalf of The Malacological Society of London, all rights reserved. Although the pond snail Lymnaea stagnalis is an emerging model organism for molecular studies in a wide variety of field... Read More about A new set of endogenous control genes for use in quantitative real-time PCR experiments show that formin Ldia2dex transcripts are enriched in the early embryo of the pond snail Lymnaea stagnalis (Panpulmonata).

The midbody interactome reveals unexpected roles for PP1 phosphatases in cytokinesis (2019)
Journal Article
Capalbo, L., Bassi, Z. I., Geymonat, M., Todesca, S., Copoiu, L., Enright, A. J., Callaini, G., Riparbelli, M. G., Yu, L., Choudhary, J. S., Ferrero, E., Wheatley, S., Douglas, M. E., Mishima, M., & D’Avino, P. P. (2019). The midbody interactome reveals unexpected roles for PP1 phosphatases in cytokinesis. Nature Communications, 10(1), Article 4513. https://doi.org/10.1038/s41467-019-12507-9

The midbody is an organelle assembled at the intercellular bridge between the two daughter cells at the end of mitosis. It controls the final separation of the daughter cells and has been involved in cell fate, polarity, tissue organization, and cili... Read More about The midbody interactome reveals unexpected roles for PP1 phosphatases in cytokinesis.

NanoBiT Complementation to Monitor Agonist-Induced Adenosine A1 Receptor Internalization (2019)
Journal Article
Soave, M., Kellam, B., Woolard, J., Briddon, S. J., & Hill, S. J. (2019). NanoBiT Complementation to Monitor Agonist-Induced Adenosine A1 Receptor Internalization. Slas Discovery, https://doi.org/10.1177/2472555219880475

Receptor internalization in response to prolonged agonist treatment is an important regulator of G protein–coupled receptor (GPCR) function. The adenosine A1 receptor (A1AR) is one of the adenosine receptor family of GPCRs, and evidence for its agoni... Read More about NanoBiT Complementation to Monitor Agonist-Induced Adenosine A1 Receptor Internalization.

Structure-Kinetic Profiling of Haloperidol Analogues at the Human Dopamine D2 Receptor (2019)
Journal Article
Fyfe, T. J., Kellam, B., Sykes, D. A., Capuano, B., Scammells, P. J., Lane, J. R., Charlton, S. J., & Mistry, S. N. (2019). Structure-Kinetic Profiling of Haloperidol Analogues at the Human Dopamine D2 Receptor. Journal of Medicinal Chemistry, 62(21), 9488-9520. https://doi.org/10.1021/acs.jmedchem.9b00864

Haloperidol is a typical antipsychotic drug (APD) associated with an increased risk of extrapyramidal side-effects (EPS) and hyperprolactinemia relative to atypical APDs such as clozapine. Both drugs are dopamine D2 receptor (D2R) antagonists, with c... Read More about Structure-Kinetic Profiling of Haloperidol Analogues at the Human Dopamine D2 Receptor.

Validating a Predictive Structure-Property Relationship by Discovery of Novel Polymers which Reduce Bacterial Biofilm Formation (2019)
Journal Article
Dundas, A. A., Sanni, O., Dubern, J.-F., Dimitrakis, G., Hook, A. L., Irvine, D. J., Williams, P., & Alexander, M. R. (2019). Validating a Predictive Structure-Property Relationship by Discovery of Novel Polymers which Reduce Bacterial Biofilm Formation. Advanced Materials, 31(49), Article 1903513. https://doi.org/10.1002/adma.201903513

ynthetic materials are an everyday component of modern healthcare yet often fail routinely as a consequence of medical‐device‐centered infections. The incidence rate for catheter‐associated urinary tract infections is between 3% and 7% for each day o... Read More about Validating a Predictive Structure-Property Relationship by Discovery of Novel Polymers which Reduce Bacterial Biofilm Formation.

Optimised insert design for improved single-molecule imaging and quantification through CRISPR-Cas9 mediated knock-in (2019)
Journal Article
Khan, A. O., White, C. W., Pike, J. A., Yule, J., Slater, A., Hill, S. J., Poulter, N. S., Thomas, S. G., & Morgan, N. V. (2019). Optimised insert design for improved single-molecule imaging and quantification through CRISPR-Cas9 mediated knock-in. Scientific Reports, 9(1), Article 14219. https://doi.org/10.1038/s41598-019-50733-9

© 2019, The Author(s). The use of CRISPR-Cas9 genome editing to introduce endogenously expressed tags has the potential to address a number of the classical limitations of single molecule localisation microscopy. In this work we present the first sys... Read More about Optimised insert design for improved single-molecule imaging and quantification through CRISPR-Cas9 mediated knock-in.

Transporting cells over several days without dry-ice (2019)
Journal Article
Wheatley, S. P., & Wheatley, D. N. (2019). Transporting cells over several days without dry-ice. Journal of Cell Science, 132(21), jcs238139. https://doi.org/10.1242/jcs.238139

This paper describes a simple, hazard-free and inexpensive procedure that allows researchers to send cultured cells across the globe at ambient temperatures. The method enables transit of up to 2 weeks without compromising cell recovery. Its use will... Read More about Transporting cells over several days without dry-ice.

Identification of key residues that regulate the interaction of kinesins with microtubule ends (2019)
Journal Article
Belsham, H. R., & Friel, C. T. (2019). Identification of key residues that regulate the interaction of kinesins with microtubule ends. Cytoskeleton, 76(7-8), 440-446. https://doi.org/10.1002/cm.21568

Kinesins are molecular motors that use energy derived from ATP turnover to walk along microtubules, or when at the microtubule end, regulate growth or shrinkage. All kinesins that regulate microtubule dynamics have long residence times at microtubule... Read More about Identification of key residues that regulate the interaction of kinesins with microtubule ends.

Plasmodium kinesin-8X associates with mitotic spindles and is essential for oocyst development during parasite proliferation and transmission (2019)
Journal Article
Zeeshan, M., Shilliday, F., Liu, T., Abel, S., Mourier, T., Ferguson, D. J. P., Rea, E., Stanway, R. R., Roques, M., Williams, D., Daniel, E., Brady, D., Roberts, A. J., Holder, A. A., Pain, A., Le, K. G., Le Roch, R. G., Moores, C. A., & Tewari, R. (2019). Plasmodium kinesin-8X associates with mitotic spindles and is essential for oocyst development during parasite proliferation and transmission. PLoS Pathogens, 15(10), Article e1008048. https://doi.org/10.1371/journal.ppat.1008048

Kinesin-8 proteins are microtubule motors that are often involved in regulation of mitotic spindle length and chromosome alignment. They move towards the plus ends of spindle microtubules and regulate the dynamics of these ends due, at least in some... Read More about Plasmodium kinesin-8X associates with mitotic spindles and is essential for oocyst development during parasite proliferation and transmission.

Testosterone therapy induces molecular programming augmenting physiological adaptations to resistance exercise in older men (2019)
Journal Article
Gharahdaghi, N., Rudrappa, S., Brook, M. S., Idris, I., Crossland, H., Hamrock, C., Aziz, H., Kadi, F., Tarum, J., Greenhaff, P. L., Constantin‐Teodosiu, D., Cegielski, J., Phillips, B. E., Wilkinson, D. J., Szewczyk, N. J., Smith, K., & Atherton, P. J. (2019). Testosterone therapy induces molecular programming augmenting physiological adaptations to resistance exercise in older men. Journal of Cachexia, Sarcopenia and Muscle, 10(6), 1276-1294. https://doi.org/10.1002/jcsm.12472

Background: The andropause is associated with declines in serum testosterone (T), loss of muscle mass (sarcopenia) and frailty. Two major interventions purported to offset sarcopenia are anabolic steroid therapies and resistance exercise training (RE... Read More about Testosterone therapy induces molecular programming augmenting physiological adaptations to resistance exercise in older men.

The impact of reduced red and processed meat consumption on cardiovascular risk factors; an intervention trial in healthy volunteers (2019)
Journal Article
Simpson, E. J., Clark, M., Razak, A. A., & Salter, A. (2019). The impact of reduced red and processed meat consumption on cardiovascular risk factors; an intervention trial in healthy volunteers. Food and Function, 2019(10), 6690-6698. https://doi.org/10.1039/C9FO00758J

Meat represents an important part of the diet for many adults, supplying essential amino acids and micronutrients. However, high red and processed meat (RPM) intake is implicated in the development of cardiovascular disease and dyslipidemia. This stu... Read More about The impact of reduced red and processed meat consumption on cardiovascular risk factors; an intervention trial in healthy volunteers.

Geographical location influences the composition of the gut microbiota in wild house mice (Mus musculus domesticus) at a fine spatial scale (2019)
Journal Article
Goertz, S., de Menezes, A. B., Birtles, R. J., Fenn, J., Lowe, A. E., MacColl, A. D., Poulin, B., Young, S., Bradley, J. E., & Taylor, C. H. (2019). Geographical location influences the composition of the gut microbiota in wild house mice (Mus musculus domesticus) at a fine spatial scale. PLoS ONE, 14(9), Article e0222501. https://doi.org/10.1371/journal.pone.0222501

The composition of the mammalian gut microbiota can be influenced by a multitude of environmental variables such as diet and infections. Studies investigating the effect of these variables on gut microbiota composition often sample across multiple se... Read More about Geographical location influences the composition of the gut microbiota in wild house mice (Mus musculus domesticus) at a fine spatial scale.

Sodium nitroprusside prevents the detrimental effects of glucose on the neurovascular unit and behaviour in zebrafish (2019)
Journal Article
Chhabria, K., Vouros, A., Gray, C., MacDonald, R. B., Jiang, Z., Wilkinson, R. N., Plant, K., Vasilaki, E., Howarth, C., & Chico, T. J. (2019). Sodium nitroprusside prevents the detrimental effects of glucose on the neurovascular unit and behaviour in zebrafish. Disease Models and Mechanisms, 12(9), Article dmm039867. https://doi.org/10.1242/dmm.039867

Diabetes is associated with dysfunction of the neurovascular unit, although the mechanisms of this are incompletely understood and currently no treatment exists to prevent these negative effects. We previously found that the nitric oxide (NO) donor s... Read More about Sodium nitroprusside prevents the detrimental effects of glucose on the neurovascular unit and behaviour in zebrafish.