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Acute Toxoplasma gondii infection in cats induced tissue-specific transcriptional response dominated by immune signatures (2018)
Journal Article
Cong, W., Dottorini, T., Khan, F., Emes, R. D., Zhang, F., Zhou, C., …Zhu, X. (2018). Acute Toxoplasma gondii infection in cats induced tissue-specific transcriptional response dominated by immune signatures. Frontiers in Immunology, 9, Article 2403. https://doi.org/10.3389/fimmu.2018.02403

RNA-sequencing was used to detect transcriptional changes in six tissues of cats, seven days after T. gondii infection. A total of 737 genes were differentially expressed (DEGs), of which 410 were up-regulated and 327 were down-regulated. The liver e... Read More about Acute Toxoplasma gondii infection in cats induced tissue-specific transcriptional response dominated by immune signatures.

Parasites and pets: a veterinary nursing guide (2018)
Book
Elsheikha, H., Wright, I. G., & McGarry, J. W. (2018). H. M. Elsheikha (Ed.). Parasites and pets: a veterinary nursing guide. CAB International Publishing. https://doi.org/10.1079/9781786394040.0000

This book, primarily focussing on parasitic diseases of cats and dogs, is designed specifically for veterinary nurses and students and adopts an enquiry based approach essential for consolidating knowledge and a deep practical understanding of this i... Read More about Parasites and pets: a veterinary nursing guide.

Immunization with a DNA vaccine cocktail encoding TgPF, TgROP16, TgROP18, TgMIC6, and TgCDPK3 genes protects mice against chronic toxoplasmosis (2018)
Journal Article
Zhang, N., Gao, Q., Wang, M., Elsheikha, H. M., Wang, B., Wang, J., …Zhu, X. (in press). Immunization with a DNA vaccine cocktail encoding TgPF, TgROP16, TgROP18, TgMIC6, and TgCDPK3 genes protects mice against chronic toxoplasmosis. Frontiers in Immunology, 9, https://doi.org/10.3389/fimmu.2018.01505

Toxoplasmosis is a zoonotic disease caused by the intracellular protozoan Toxoplasma gondii; and a major source of infection in humans is via ingestion of T. gondii tissue cysts. Ultimately, the goal of anti-toxoplasmosis vaccines is to elicit a sust... Read More about Immunization with a DNA vaccine cocktail encoding TgPF, TgROP16, TgROP18, TgMIC6, and TgCDPK3 genes protects mice against chronic toxoplasmosis.

Transcriptomic analysis reveals Toxoplasma gondii strain-specific differences in host cell response to dense granule protein GRA15 (2018)
Journal Article
Liu, Q., Gao, W., Elsheikha, H. M., He, J., Li, F., Yang, W., & Zhu, X. (2018). Transcriptomic analysis reveals Toxoplasma gondii strain-specific differences in host cell response to dense granule protein GRA15. Parasitology Research, 117(9), 2785–2793. https://doi.org/10.1007/s00436-018-5966-8

Growth and replication of the protozoan parasite Toxoplasma gondii within host cell entail the production of several effector proteins, which the parasite exploits for counteracting the host’s immune response. Despite considerable research to define... Read More about Transcriptomic analysis reveals Toxoplasma gondii strain-specific differences in host cell response to dense granule protein GRA15.

Toxocariasis: a silent threat with a progressive public health impact (2018)
Journal Article
Chen, J., Liu, Q., Liu, G., Zheng, W., Hong, S., Sugiyama, H., …Elsheikha, H. M. (in press). Toxocariasis: a silent threat with a progressive public health impact. Infectious Diseases of Poverty, 7(1), https://doi.org/10.1186/s40249-018-0437-0

Background: Toxocariasis is a neglected parasitic zoonosis that afflicts millions of the pediatric and adolescent populations worldwide, especially in impoverished communities. This disease is caused by infection with the larvae of Toxocara canis and... Read More about Toxocariasis: a silent threat with a progressive public health impact.

A novel recombinase polymerase amplification (RPA) assay for the rapid isothermal detection of Neospora caninum in aborted bovine fetuses (2018)
Journal Article
Tian, A., Elsheikha, H. M., Zhou, D., Wu, Y., Chen, M., Wang, M., …Zhu, X. (2018). A novel recombinase polymerase amplification (RPA) assay for the rapid isothermal detection of Neospora caninum in aborted bovine fetuses. Veterinary Parasitology, 258, https://doi.org/10.1016/j.vetpar.2018.06.004

The development of a method to rapidly diagnose Neospora caninum infection is highly desirable. Recombinase polymerase amplification (RPA), combined with lateral flow (LF) strips, is a novel approach to rapidly amplify and visualize DNA. We have deve... Read More about A novel recombinase polymerase amplification (RPA) assay for the rapid isothermal detection of Neospora caninum in aborted bovine fetuses.

Live-attenuated Pru: Δcdpk2 strain of Toxoplasma gondii protects against acute, chronic and congenital toxoplasmosis (2018)
Journal Article
Wang, J., Li, T., Elsheikha, H. M., Chen, K., Cong, W., Yang, W., …Zhu, X. (2018). Live-attenuated Pru: Δcdpk2 strain of Toxoplasma gondii protects against acute, chronic and congenital toxoplasmosis. Journal of Infectious Diseases, 218(5), 768–777. https://doi.org/10.1093/infdis/jiy211

Background: The threat of Toxoplasma gondii infection in immunocompromised individuals and pregnant women necessitates the development of a safe and effective vaccine. Here, we examined the immune protection conferred by live-attenuated strain of T.... Read More about Live-attenuated Pru: Δcdpk2 strain of Toxoplasma gondii protects against acute, chronic and congenital toxoplasmosis.

Holographic optical trapping Raman micro-spectroscopy for interacting live cells (2018)
Working Paper
Sinjab, F., Elsheikha, H. M., Awuah, D., Gibson, G., Padgett, M., Ghaemmaghami, A. M., & Notingher, I. Holographic optical trapping Raman micro-spectroscopy for interacting live cells

We present a combined holographic optical tweezers and confocal Raman spectroscopy instrument which allows fast, flexible, and interactive manipulation with molecular measurement of interacting live cell systems. Multiple laser foci created using a s... Read More about Holographic optical trapping Raman micro-spectroscopy for interacting live cells.

A recombinant Fasciola gigantica 14-3-3 epsilon protein (rFg14-3-3e) modulates various functions of goat peripheral blood mononuclear cells (2018)
Journal Article
Tian, A., Lu, M., Calderón-Mantilla, G., Petsalaki, E., Dottorini, T., Tian, X., …Zhu, X. (2018). A recombinant Fasciola gigantica 14-3-3 epsilon protein (rFg14-3-3e) modulates various functions of goat peripheral blood mononuclear cells. Parasites and Vectors, 11(1), https://doi.org/10.1186/s13071-018-2745-4

Background The molecular structure of Fasciola gigantica 14-3-3 protein has been characterized. However, the involvement of this protein in parasite pathogenesis remains elusive and its effect on the functions of innate immune cells is unknown. We... Read More about A recombinant Fasciola gigantica 14-3-3 epsilon protein (rFg14-3-3e) modulates various functions of goat peripheral blood mononuclear cells.

Identification of two novel host proteins interacting with Toxoplasma gondii 14-3-3 protein by yeast two-hybrid system (2018)
Journal Article
Li, F., Liu, Q., Elsheikha, H. M., Yang, W., Hou, J., & Zhu, X. (in press). Identification of two novel host proteins interacting with Toxoplasma gondii 14-3-3 protein by yeast two-hybrid system. Parasitology Research, https://doi.org/10.1007/s00436-018-5812-z

Toxoplasma gondii deploys many effector proteins in order to hijack and manipulate host cell signaling pathways, allowing parasite colonization, subversion of immune responses, and disease progression. T. gondii effector protein 14-3-3 (Tg14-3-3) pro... Read More about Identification of two novel host proteins interacting with Toxoplasma gondii 14-3-3 protein by yeast two-hybrid system.

Novel Entamoeba findings in nonhuman primates (2018)
Journal Article
Elsheikha, H. M., Regan, C. S., & Clark, C. G. (2018). Novel Entamoeba findings in nonhuman primates. Trends in Parasitology, 34(4), https://doi.org/10.1016/j.pt.2017.12.008

In addition to well-known human-infecting species, Entamoeba species not found in humans have been identified recently in nonhuman primates (NHPs). Importantly, it has become clear that the organism identified as Entamoeba histolytica in NHPs is usua... Read More about Novel Entamoeba findings in nonhuman primates.

Serum metabolic profiling of oocyst-induced Toxoplasma gondii acute and chronic infections in mice using mass-spectrometry (2018)
Journal Article
Zhou, C., Cong, W., Chen, X., He, S., Elsheikha, H. M., & Zhu, X. (2018). Serum metabolic profiling of oocyst-induced Toxoplasma gondii acute and chronic infections in mice using mass-spectrometry. Frontiers in Microbiology, 8, https://doi.org/10.3389/fmicb.2017.02612

Toxoplasma gondii is an obligate intracellular parasite causing severe diseases in immunocompromised individuals and congenitally infected neonates, such as toxoplasmosis encephalitis and toxoplasmic chorioretinitis. This study aimed to determine whe... Read More about Serum metabolic profiling of oocyst-induced Toxoplasma gondii acute and chronic infections in mice using mass-spectrometry.