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Acute Toxoplasma gondii infection in cats induced tissue-specific transcriptional response dominated by immune signatures

Cong, Wei; Dottorini, Tania; Khan, Faraz; Emes, Richard D.; Zhang, Fu-Kai; Zhou, Chun-Xue; He, Jun-Jun; Zhang, Xiao-Xuan; Elsheikha, Hany M.; Zhu, Xing-Quan

Acute Toxoplasma gondii infection in cats induced tissue-specific transcriptional response dominated by immune signatures Thumbnail


Authors

Wei Cong

Faraz Khan

Richard D. Emes

Fu-Kai Zhang

Chun-Xue Zhou

Jun-Jun He

Xiao-Xuan Zhang

Xing-Quan Zhu



Abstract

RNA-sequencing was used to detect transcriptional changes in six tissues of cats, seven days after T. gondii infection. A total of 737 genes were differentially expressed (DEGs), of which 410 were up-regulated and 327 were down-regulated. The liver exhibited 151 DEGs, lung (149 DEGs), small intestine (130 DEGs), heart (123 DEGs), brain (104 DEGs), and spleen (80 DEGs)-suggesting tissue-specific transcriptional patterns. Gene ontology and KEGG analyses identified DEGs enriched in immune pathways, such as cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, NOD-like receptor signaling pathway, NF-kappa B signaling pathway, MAPK signaling pathway,
T cell receptor signaling pathway, and the cytosolic DNA sensing pathway. C-X-C motif chemokine 10 (CXCL10) was involved in most of the immune-related pathways. PI3K/Akt expression was down-regulated in all tissues, except the spleen. The genes for phosphatase, indoleamine 2,3-dioxygenase, Hes Family BHLH Transcription Factor 1, and guanylate-binding protein 5, playing various roles in immune defense, were co-expressed across various feline tissues. Multivariate K-means clustering analysis produced seven gene clusters featuring similar gene expression patterns specific to individual tissues, with lung tissue cluster having the largest number of DEGs. These findings suggest the presence of a broad immune defense mechanism across various tissues in cats against acute T. gondii infection.

Journal Article Type Article
Acceptance Date Sep 28, 2018
Online Publication Date Oct 19, 2018
Publication Date Oct 19, 2018
Deposit Date Oct 22, 2018
Publicly Available Date Oct 22, 2018
Journal Frontiers in Immunology
Electronic ISSN 1664-3224
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 9
Article Number 2403
DOI https://doi.org/10.3389/fimmu.2018.02403
Public URL https://nottingham-repository.worktribe.com/output/1179025
Publisher URL https://www.frontiersin.org/articles/10.3389/fimmu.2018.02403/full

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