Holographic optical trapping Raman micro-spectroscopy for interacting live cells

Sinjab, Faris; Elsheikha, Hany M.; Awuah, Dennis; Gibson, Graham; Padgett, Miles; Ghaemmaghami, Amir M.; Notingher, Ioan

Authors

Faris Sinjab

Professor HANY ELSHEIKHA hany.elsheikha@nottingham.ac.uk
Professor of Interdisciplinary Parasitology

Dennis Awuah

Graham Gibson

Miles Padgett

Professor AMIR GHAEMMAGHAMI AMIR.GHAEMMAGHAMI@NOTTINGHAM.AC.UK
Professor of Immunology and Immuno- Bioengineering

IOAN NOTINGHER ioan.notingher@nottingham.ac.uk
Professor of Physics

Abstract

We present a combined holographic optical tweezers and confocal Raman spectroscopy instrument which allows fast, flexible, and interactive manipulation with molecular measurement of interacting live cell systems. Multiple laser foci created using a spatial light modulator are simultaneously used for optical trapping and spontaneous Raman spectroscopy. To enable confocal Raman measurements with high spectral resolution, a digital micro-mirror device was used to generate reflective pinholes which are matched to each laser trap. We demonstrate this unique capability by initiating complex interactions between multiple live cells whilst non-invasively acquiring Raman spectra of the processes with high spatial, spectral, and temporal resolution.

Citation

Sinjab, F., Elsheikha, H. M., Awuah, D., Gibson, G., Padgett, M., Ghaemmaghami, A. M., & Notingher, I. Holographic optical trapping Raman micro-spectroscopy for interacting live cells

Deposit Date	Jul 26, 2022
Publicly Available Date	Jul 26, 2022
Publisher	Optical Society of America
Public URL	https://nottingham-repository.worktribe.com/output/1882113
Publisher URL	https://www.biorxiv.org/content/10.1101/292334v1