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A conserved set of maternal genes? Insights from a molluscan transcriptome (2014)
Journal Article
Liu, M. M., Davey, J. W., Jackson, D. J., Blaxter, M. L., & Davison, A. (2014). A conserved set of maternal genes? Insights from a molluscan transcriptome. International Journal of Developmental Biology, 58, https://doi.org/10.1387/ijdb.140121ad

The early animal embryo is entirely reliant on maternal gene products for a ‘jump-start’ that transforms a transcriptionally inactive embryo into a fully functioning zygote. Despite extensive work on model species, it has not been possible to perform... Read More about A conserved set of maternal genes? Insights from a molluscan transcriptome.

The ORF4b-encoded accessory proteins of Middle East respiratory syndrome coronavirus and two related bat coronaviruses localize to the nucleus and inhibit innate immune signalling (2014)
Journal Article
Matthews, K. L., Coleman, C. M., van der Meer, Y., Snijder, E. J., & Frieman, M. B. (2014). The ORF4b-encoded accessory proteins of Middle East respiratory syndrome coronavirus and two related bat coronaviruses localize to the nucleus and inhibit innate immune signalling. Journal of General Virology, 95(4), 874-882. https://doi.org/10.1099/vir.0.062059-0

The recently emerged Middle East respiratory syndrome coronavirus (MERS-CoV), a betacoronavirus, is associated with severe pneumonia and renal failure. The environmental origin of MERS-CoV is as yet unknown; however, its genome sequence is closely re... Read More about The ORF4b-encoded accessory proteins of Middle East respiratory syndrome coronavirus and two related bat coronaviruses localize to the nucleus and inhibit innate immune signalling.

Validation of N-myristoyltransferase as an antimalarial drug target using an integrated chemical biology approach (2013)
Journal Article

Malaria is an infectious disease caused by parasites of the genus Plasmodium, which leads to approximately one million deaths per annum worldwide. Chemical validation of new antimalarial targets is urgently required in view of rising resistance to cu... Read More about Validation of N-myristoyltransferase as an antimalarial drug target using an integrated chemical biology approach.

Poly(Acrylic Acid)–Poly(Ethylene Glycol) Nanoparticles Designed for Ophthalmic Drug Delivery (2013)
Journal Article
Vasi, A.-M., Popa, M. I., Tanase, E. C., Butnaru, M., & Verestiuc, L. (2014). Poly(Acrylic Acid)–Poly(Ethylene Glycol) Nanoparticles Designed for Ophthalmic Drug Delivery. Journal of Pharmaceutical Sciences, 103(2), 676-686. https://doi.org/10.1002/jps.23793

Poly(acrylic acid) (PAA) and poly(ethylene glycol) (PEG), four-arm, amine-terminated particles with nanometer size and spherical shape were obtained by the polymers cross-linking, via activation with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hy... Read More about Poly(Acrylic Acid)–Poly(Ethylene Glycol) Nanoparticles Designed for Ophthalmic Drug Delivery.

Kinetics and mechanism of G protein-coupled receptor activation (2013)
Journal Article
Lohse, M. J., Maiellaro, I., & Calebiro, D. (2014). Kinetics and mechanism of G protein-coupled receptor activation. Current Opinion in Cell Biology, 27, 87-93. https://doi.org/10.1016/j.ceb.2013.11.009

The activation of a G protein-coupled receptor is generally triggered by binding of an agonist to the receptor's binding pocket, or, in the case of rhodopsin, by light-induced changes of the pre-bound retinal. This is followed by a series of a confor... Read More about Kinetics and mechanism of G protein-coupled receptor activation.

Investigating the microrheology of the vitreous humor using an optically trapped local probe (2013)
Journal Article
Watts, F., Tan, L. E., Wilson, C. G., Girkin, J. M., Tassieri, M., & Wright, A. J. (2013). Investigating the microrheology of the vitreous humor using an optically trapped local probe. Journal of Optics, 16(1), https://doi.org/10.1088/2040-8978/16/1/015301

We demonstrate that an optically trapped silica bead can be used as a local probe to measure the micro-rheology of the vitreous humor. The Brownian motion of the bead was observed using a fast camera and the micro-rheology determined by analysis of t... Read More about Investigating the microrheology of the vitreous humor using an optically trapped local probe.

Investigation of the functional expression of purine and pyrimidine receptors in porcine isolated pancreatic arteries (2013)
Journal Article
Alsaqati, M., Chan, S., & Ralevic, V. (2014). Investigation of the functional expression of purine and pyrimidine receptors in porcine isolated pancreatic arteries. Purinergic Signalling, 10(2),

Receptors for purines and pyrimidines are expressed throughout the cardiovascular system. This study investigated their functional expression in porcine isolated pancreatic arteries. Pancreatic arteries (endothelium intact or denuded) were prepared f... Read More about Investigation of the functional expression of purine and pyrimidine receptors in porcine isolated pancreatic arteries.

Hyperactive mariner transposons are created by mutations that disrupt allosterism and increase the rate of transposon end synapsis (2013)
Journal Article
Liu, D., & Chalmers, R. (2014). Hyperactive mariner transposons are created by mutations that disrupt allosterism and increase the rate of transposon end synapsis. Nucleic Acids Research, 42(4), https://doi.org/10.1093/nar/gkt1218

New applications for transposons in vertebrate genetics have spurred efforts to develop hyperactive variants. Typically, a genetic screen is used to identify several hyperactive point mutations, which are then incorporated in a single transposase gen... Read More about Hyperactive mariner transposons are created by mutations that disrupt allosterism and increase the rate of transposon end synapsis.

Post-processing of polymer foam tissue scaffolds with high power ultrasound: A route to increased pore interconnectivity, pore size and fluid transport (2013)
Journal Article
Watson, N. J., Johal, R. K., Glover, Z., Reinwald, Y., White, L. J., Ghaemmaghami, A. M., …Parker, N. G. (2013). Post-processing of polymer foam tissue scaffolds with high power ultrasound: A route to increased pore interconnectivity, pore size and fluid transport. Materials Science and Engineering: C, 33(8), 4825-4832. https://doi.org/10.1016/j.msec.2013.07.046

The aimof this work is to demonstrate that the structural and fluidic properties of polymer foamtissue scaffolds, post-fabrication but prior to the introduction of cells, can be engineered via exposure to high power ultrasound. Our analysis is suppor... Read More about Post-processing of polymer foam tissue scaffolds with high power ultrasound: A route to increased pore interconnectivity, pore size and fluid transport.

Quantifying the range of a lipid phosphate signal in vivo (2013)
Journal Article
Mukherjee, A., Neher, R. A., & Renault, A. D. (2013). Quantifying the range of a lipid phosphate signal in vivo. Journal of Cell Science, 126(23), 5453-5464. https://doi.org/10.1242/jcs.136176

Quantitative information about the range of influence of extracellular signaling molecules is critical for understanding their effects, but is difficult to determine in the complex and dynamic three-dimensional environment of a living embryo. Drosoph... Read More about Quantifying the range of a lipid phosphate signal in vivo.

Neuron-glia crosstalk in health and disease: fractalkine and CX3CR1 take centre stage (2013)
Journal Article
Sheridan, G. K., & Murphy, K. J. (2013). Neuron-glia crosstalk in health and disease: fractalkine and CX3CR1 take centre stage. Open Biology, 3(12), Article 130181. https://doi.org/10.1098/rsob.130181

An essential aspect of normal brain function is the bidirectional interaction and communication between neurons and neighbouring glial cells. To this end, the brain has evolved ligand-receptor partnerships that facilitate crosstalk between different... Read More about Neuron-glia crosstalk in health and disease: fractalkine and CX3CR1 take centre stage.

Monoclonal anti-β1-adrenergic receptor antibodies activate G protein signaling in the absence of β-arrestin recruitment (2013)
Journal Article
Hutchings, C. J., Cseke, G., Osborne, G., Woolard, J., Zhukov, A., Koglin, M., …Marshall, F. H. (2014). Monoclonal anti-β1-adrenergic receptor antibodies activate G protein signaling in the absence of β-arrestin recruitment. mAbs, 6(1), 246-261. https://doi.org/10.4161/mabs.27226

Thermostabilized G protein-coupled receptors used as antigens for in vivo immunization have resulted in the generation of functional agonistic anti-β1-adrenergic (β1AR) receptor monoclonal antibodies (mAbs). The focus of this study was to examine the... Read More about Monoclonal anti-β1-adrenergic receptor antibodies activate G protein signaling in the absence of β-arrestin recruitment.

miR-24 affects hair follicle morphogenesis targeting Tcf-3 (2013)
Journal Article
Amelio, I., Lena, A. M., Bonanno, E., Melino, G., & Candi, E. (2013). miR-24 affects hair follicle morphogenesis targeting Tcf-3. Cell Death and Disease, 4(11), Article e922. https://doi.org/10.1038/cddis.2013.426

During embryonic development, hair follicles (HFs) develop from an epidermal–mesenchymal cross talk between the ectoderm progenitor layer and the underlying dermis. Epidermal stem cell activation represents a crucial point both for HF morphogenesis a... Read More about miR-24 affects hair follicle morphogenesis targeting Tcf-3.

A structure-activity analysis of biased agonism at the dopamine D2 receptor. (2013)
Journal Article
Shonberg, J., Herenbrink, C. K., López, L., Christopoulos, A., Scammells, P. J., Capuano, B., & Lane, J. R. (2013). A structure-activity analysis of biased agonism at the dopamine D2 receptor. Journal of Medicinal Chemistry, 56(22), 9199-9221. https://doi.org/10.1021/jm401318w

Biased agonism offers an opportunity for the medicinal chemist to discover pathway-selective ligands for GPCRs. A number of studies have suggested that biased agonism at the dopamine D2 receptor (D2R) may be advantageous for the treatment of neuropsy... Read More about A structure-activity analysis of biased agonism at the dopamine D2 receptor..

Self-reporting Scaffolds for 3-Dimensional Cell Culture (2013)
Journal Article
Harrington, H., Rose, F. R., Aylott, J. W., & Ghaemmaghami, A. M. (2013). Self-reporting Scaffolds for 3-Dimensional Cell Culture. Journal of Visualized Experiments, https://doi.org/10.3791/50608

Culturing cells in 3D on appropriate scaffolds is thought to better mimic the in vivo microenvironment and increase cell-cell interactions. The resulting 3D cellular construct can often be more relevant to studying the molecular events and cell-cell... Read More about Self-reporting Scaffolds for 3-Dimensional Cell Culture.

Increased function of pronociceptive TRPV1 at the level of the joint in a rat model of osteoarthritis pain (2013)
Journal Article
Kelly, S., Chapman, R., Woodhams, S., Sagar, D. R., Turner, J., Burston, J., …Chapman, V. (2014). Increased function of pronociceptive TRPV1 at the level of the joint in a rat model of osteoarthritis pain. Annals of the Rheumatic Diseases, 74, https://doi.org/10.1136/annrheumdis-2013-203413

Objectives Blockade of transient receptor potential vanilloid 1 (TRPV1) with systemic antagonists attenuates osteoarthritis (OA) pain behaviour in rat models, but on-target-mediated hyperthermia has halted clinical trials. The present study investiga... Read More about Increased function of pronociceptive TRPV1 at the level of the joint in a rat model of osteoarthritis pain.

Accurate measurement of gene copy number for human alpha-defensin DEFA1A3 (2013)
Journal Article
Khan, F. F., Carpenter, D., Mitchell, L., Mansouri, O., Black, H. A., Tyson, J., & Armour, J. A. (2013). Accurate measurement of gene copy number for human alpha-defensin DEFA1A3. BMC Genomics, 14(719), https://doi.org/10.1186/1471-2164-14-719

Background: Multi-allelic copy number variants include examples of extensive variation between individuals in the copy number of important genes, most notably genes involved in immune function. The definition of this variation, and analysis of its i... Read More about Accurate measurement of gene copy number for human alpha-defensin DEFA1A3.