Self-reporting Scaffolds for 3-Dimensional Cell Culture

Harrington, Helen; Rose, Felicity R.A.J.; Aylott, Jonathan W.; Ghaemmaghami, Amir M.

Abstract

Culturing cells in 3D on appropriate scaffolds is thought to better mimic the in vivo microenvironment and increase cell-cell interactions. The resulting 3D cellular construct can often be more relevant to studying the molecular events and cell-cell interactions than similar experiments studied in 2D. To create effective 3D cultures with high cell viability throughout the scaffold the culture conditions such as oxygen and pH need to be carefully controlled as gradients in analyte concentration can exist throughout the 3D construct. Here we describe the methods of preparing biocompatible pH responsive sol-gel nanosensors and their incorporation into poly(lactic-co-glycolic acid) (PLGA) electrospun scaffolds along with their subsequent preparation for the culture of mammalian cells. The pH responsive scaffolds can be used as tools to determine microenvironmental pH within a 3D cellular construct. Furthermore, we detail the delivery of pH responsive nanosensors to the intracellular environment of mammalian cells whose growth was supported by electrospun PLGA scaffolds. The cytoplasmic location of the pH responsive nanosensors can be utilized to monitor intracellular pH (pHi) during ongoing experimentation.

Citation

Harrington, H., Rose, F. R., Aylott, J. W., & Ghaemmaghami, A. M. (2013). Self-reporting Scaffolds for 3-Dimensional Cell Culture. Journal of Visualized Experiments, https://doi.org/10.3791/50608

• Journal Article Type: Article
• Online Publication Date: Nov 7, 2013
• Publication Date: Nov 7, 2013
• Deposit Date: Jan 3, 2023
• Electronic ISSN: 1940-087X
• Publisher: Journal of Visualized Experiments
• Peer Reviewed: Peer Reviewed
• Issue: 81
• DOI: https://doi.org/10.3791/50608
• Keywords: General Immunology and Microbiology; General Biochemistry, Genetics and Molecular Biology; General Chemical Engineering; General Neuroscience
• Public URL: https://nottingham-repository.worktribe.com/output/3097464
• Publisher URL: https://www.jove.com/v/50608/self-reporting-scaffolds-for-3-dimensional-cell-culture