miR-24 affects hair follicle morphogenesis targeting Tcf-3

Amelio, I; Lena, A M; Bonanno, E; Melino, G; Candi, E

Abstract

During embryonic development, hair follicles (HFs) develop from an epidermal–mesenchymal cross talk between the ectoderm progenitor layer and the underlying dermis. Epidermal stem cell activation represents a crucial point both for HF morphogenesis and for hair regeneration. miR-24 is an anti-proliferative microRNA (miRNA), which is induced during differentiation of several cellular systems including the epidermis. Here, we show that miR-24 is expressed in the HF and has a role in hair morphogenesis. We generated transgenic mice ectopically expressing miR-24 under the K5 promoter. The K5::miR-24 animals display a marked defect in HF morphogenesis, with thinning of hair coat and altered HF structure. Expression of miR-24 alters the normal process of hair keratinocyte differentiation, leading to altered expression of differentiation markers. MiR-24 directly represses the hair keratinocyte stemness regulator Tcf-3. These results support the notion that microRNAs, and among them miR-24, have an important role in postnatal epidermal homeostasis.

• Journal Article Type: Article
• Acceptance Date: Sep 30, 2013
• Online Publication Date: Nov 14, 2013
• Publication Date: 2013-11
• Deposit Date: Apr 1, 2020
• Journal: Cell Death & Disease
• Print ISSN: 2041-4889
• Publisher: Springer Nature [academic journals on nature.com]
• Peer Reviewed: Peer Reviewed
• Volume: 4
• Issue: 11
• Article Number: e922
• DOI: https://doi.org/10.1038/cddis.2013.426
• Public URL: https://nottingham-repository.worktribe.com/output/4237224
• Publisher URL: https://www.nature.com/articles/cddis2013426