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Increased function of pronociceptive TRPV1 at the level of the joint in a rat model of osteoarthritis pain

Kelly, Sara; Chapman, R.J.; Woodhams, S.; Sagar, Devi Rani; Turner, J.; Burston, J.J.; Bullock, C.; Paton, K.; Huang, J.; Wong, A.; McWilliams, D.F.; Okine, B.N.; Barrett, D.A.; Hathway, G.J.; Walsh, D.A.; Chapman, V.

Authors

Sara Kelly

R.J. Chapman

S. Woodhams

Devi Rani Sagar

J. Turner

J.J. Burston

C. Bullock

K. Paton

J. Huang

A. Wong

D.F. McWilliams

B.N. Okine

D.A. Barrett

G.J. Hathway

D.A. Walsh

V. Chapman



Abstract

Objectives Blockade of transient receptor potential vanilloid 1 (TRPV1) with systemic antagonists attenuates osteoarthritis (OA) pain behaviour in rat models, but on-target-mediated hyperthermia has halted clinical trials. The present study investigated the potential for targeting TRPV1 receptors within the OA joint in order to produce analgesia.
Methods The presence of TRPV1 receptors in human synovium was detected using western blotting and immunohistochemistry. In a rat model of OA, joint levels of an endogenous ligand for TRPV1, 12- ydroxyeicosatetraenoic acid (12-HETE), were quantified using liquid chromatography-tandem mass spectrometry (LCMS/MS). Effects of peripheral administration of the TRPV1 receptor antagonist JNJ-17203212 on afferent fibre activity, pain behaviour and core body temperature were investigated. Effects of a spinal administration of JNJ-17203212 on dorsal horn neuronal responses were studied.
Results We demonstrate increased TRPV1 immunoreactivity in human OA synovium, confirming the diseased joint as a potential therapeutic target for TRPV1-mediated analgesia. In a model of OA pain, we report increased joint levels of 12-HETE, and the sensitisation of joint afferent neurones to mechanical
stimulation of the knee. Local administration of JNJ- 17203212 reversed this sensitisation of joint afferents
and inhibited pain behaviour (weight-bearing asymmetry), to a comparable extent as systemic JNJ-
17203212, in this model of OA pain, but did not alter core body temperature. There was no evidence for
increased TRPV1 function in the spinal cord in this model of OA pain.
Conclusions Our data provide a clinical and mechanistic rationale for the future investigation of the therapeutic benefits of intra-articular administration of TRPV1 antagonists for the treatment of OA pain.

Journal Article Type Article
Publication Date Dec 4, 2014
Journal Annals of Rheumatic Disease
Print ISSN 0003-4967
Electronic ISSN 1468-2060
Publisher BMJ Publishing Group
Peer Reviewed Peer Reviewed
Volume 74
Institution Citation Kelly, S., Chapman, R., Woodhams, S., Sagar, D. R., Turner, J., Burston, J., ā€¦Chapman, V. (2014). Increased function of pronociceptive TRPV1 at the level of the joint in a rat model of osteoarthritis pain. Annals of the Rheumatic Diseases, 74, doi:10.1136/annrheumdis-2013-203413
DOI https://doi.org/10.1136/annrheumdis-2013-203413
Publisher URL http://ard.bmj.com/content/74/1/252
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc/4.0





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