All Outputs (33)

Investigating the intracellular effects of hyperbranched polycation-DNA complexes on lung cancer cells using LC-MS-based metabolite profiling (2019)
Journal Article
Alazzo, A., Al-Natour, M., Spriggs, K., Stolnik, S., Ghaemmaghami, A., Kim, D., & Alexander, C. (2019). Investigating the intracellular effects of hyperbranched polycation-DNA complexes on lung cancer cells using LC-MS-based metabolite profiling. Molecular Omics, 15(1), 77-87. https://doi.org/10.1039/C8MO00139A

Cationic polymers have emerged as a promising alternative to viral vectors in gene therapy. They are cheap to scale up, easy to functionalise and also presume to be safer than the viral vectors, however many of them are cytotoxic. The large number of... Read More about Investigating the intracellular effects of hyperbranched polycation-DNA complexes on lung cancer cells using LC-MS-based metabolite profiling.

New generation of bioreactors that advance extracellular matrix modelling and tissue engineering (2018)
Journal Article
Ahmed, S., Chauhan, V. M., Ghaemmaghami, A. M., & Aylott, J. W. (2019). New generation of bioreactors that advance extracellular matrix modelling and tissue engineering. Biotechnology Letters, 41(1), 1–25. https://doi.org/10.1007/s10529-018-2611-7

Bioreactors hold a lot of promise for tissue engineering and regenerative medicine applications. They have multiple uses including cell cultivation for therapeutic production and for in vitro organ modelling to provide a more physiologically relevant... Read More about New generation of bioreactors that advance extracellular matrix modelling and tissue engineering.

Human dendritic cell sequestration onto the Necator americanus larval sheath during ex-sheathing: a possible mechanism for immune privilege (2018)
Journal Article
Hassan, A., Pritchard, D. I., & Ghaemmaghami, A. M. (2018). Human dendritic cell sequestration onto the Necator americanus larval sheath during ex-sheathing: a possible mechanism for immune privilege. Parasitology, 145(9), 1183-1190. https://doi.org/10.1017/S0031182018000136

Despite the profound health implications of Necator americanus (N. americanus) infection in humans, many aspects of its interaction with the host immune system are poorly understood. Here we investigated the early events at the interface of N. americ... Read More about Human dendritic cell sequestration onto the Necator americanus larval sheath during ex-sheathing: a possible mechanism for immune privilege.

Image based machine learning for identification of macrophage subsets (2017)
Journal Article
Rostam, H., Reynolds, P. M., Alexander, M. R., Gadegaard, N., & Ghaemmaghami, A. M. (2017). Image based machine learning for identification of macrophage subsets. Scientific Reports, 7(1), Article 3521. https://doi.org/10.1038/s41598-017-03780-z

Macrophages play a crucial rule in orchestrating immune responses against pathogens and foreign materials. Macrophages have remarkable plasticity in response to environmental cues and are able to acquire a spectrum of activation status, best exemplif... Read More about Image based machine learning for identification of macrophage subsets.

Impact of surface chemistry and topography on the function of antigen presenting cells (2015)
Journal Article
Rostam, H. M., Singh, S., Vrana, N. E., Alexander, M. R., & Ghaemmaghami, A. M. (2015). Impact of surface chemistry and topography on the function of antigen presenting cells. Biomaterials Science, 3(3), 424-441. https://doi.org/10.1039/c4bm00375f

Antigen presenting cells (APCs) such as macrophages and dendritic cells (DCs) play a crucial role in orchestrating immune responses against foreign materials. The activation status of APCs can determine the outcome of an immune response following imp... Read More about Impact of surface chemistry and topography on the function of antigen presenting cells.

Utilizing nanosensor-incorporated scaffolds in the development of a 3D lung model (2014)
Book Chapter
Harrington, H. C., Rose, F. R., Ghaemmaghami, A. M., & Aylott, J. W. (2014). Utilizing nanosensor-incorporated scaffolds in the development of a 3D lung model. In J. Haycock, A. Ahluwalia, & J. M. Wilkinson (Eds.), Cellular In Vitro Testing (89-107). Jenny Stanford Publishing

Human airway smooth muscle maintain in situ cell orientation and phenotype when cultured on aligned electrospun scaffolds (2014)
Journal Article
Morris, G., Bridge, J., Eltboli, O., Lewis, M., Knox, A., Aylott, J. W., …Rose, F. R. (2014). Human airway smooth muscle maintain in situ cell orientation and phenotype when cultured on aligned electrospun scaffolds. AJP - Lung Cellular and Molecular Physiology, 307(1), Article L38-L47. https://doi.org/10.1152/ajplung.00318.2013

Human airway smooth muscle (HASM) contraction plays a central role in regulating airway resistance in both healthy and asthmatic bronchioles. In vitro studies that investigate the intricate mechanisms that regulate this contractile process are predom... Read More about Human airway smooth muscle maintain in situ cell orientation and phenotype when cultured on aligned electrospun scaffolds.

A novel electrospun biphasic scaffold provides optimal three-dimensional topography forin vitroco-culture of airway epithelial and fibroblast cells (2014)
Journal Article
Morris, G., Bridge, J., Brace, L., Knox, A., Aylott, J. W., Brightling, C., …Rose, F. R. (2014). A novel electrospun biphasic scaffold provides optimal three-dimensional topography forin vitroco-culture of airway epithelial and fibroblast cells. Biofabrication, 6(3), Article 035014. https://doi.org/10.1088/1758-5082/6/3/035014

Conventional airway in vitro models focus upon the function of individual structural cells cultured in a two-dimensional monolayer, with limited three-dimensional (3D) models of the bronchial mucosa. Electrospinning offers an attractive method to pro... Read More about A novel electrospun biphasic scaffold provides optimal three-dimensional topography forin vitroco-culture of airway epithelial and fibroblast cells.

Immunocompetent 3D model of human upper airway for disease modeling and in vitro drug evaluation (2014)
Journal Article
Harrington, H., Cato, P., Salazar, F., Wilkinson, M., Knox, A., Haycock, J. W., …Ghaemmaghami, A. M. (2014). Immunocompetent 3D model of human upper airway for disease modeling and in vitro drug evaluation. Molecular Pharmaceutics, 11(7), 2082-2091. https://doi.org/10.1021/mp5000295

The development of more complex in vitro models for the assessment of novel drugs and chemicals is needed because of the limited biological relevance of animal models to humans as well as ethical considerations. Although some human-cell-based assays... Read More about Immunocompetent 3D model of human upper airway for disease modeling and in vitro drug evaluation.

Self-reporting Scaffolds for 3-Dimensional Cell Culture (2013)
Journal Article
Harrington, H., Rose, F. R., Aylott, J. W., & Ghaemmaghami, A. M. (2013). Self-reporting Scaffolds for 3-Dimensional Cell Culture. Journal of Visualized Experiments, https://doi.org/10.3791/50608

Culturing cells in 3D on appropriate scaffolds is thought to better mimic the in vivo microenvironment and increase cell-cell interactions. The resulting 3D cellular construct can often be more relevant to studying the molecular events and cell-cell... Read More about Self-reporting Scaffolds for 3-Dimensional Cell Culture.

Interconnectivity and permeability of supercritical fluid-foamed scaffolds and the effect of their structural properties on cell distribution (2013)
Journal Article
Reinwald, Y., Johal, R. K., Ghaemmaghami, A. M., Rose, F. R., Howdle, S. M., & Shakesheff, K. M. (2014). Interconnectivity and permeability of supercritical fluid-foamed scaffolds and the effect of their structural properties on cell distribution. Polymer, 55(1), 435-444. https://doi.org/10.1016/j.polymer.2013.09.041

This study aims to investigate interconnectivity and permeability of scCO2-foamed scaffolds and the influence of structural scaffold properties on cell distribution. Supercritical fluid technology was utilized to fabricated scaffolds from 37 kDa, 53... Read More about Interconnectivity and permeability of supercritical fluid-foamed scaffolds and the effect of their structural properties on cell distribution.

Tissue transglutaminase (TG-2) modified amniotic membrane: A novel scaffold for biomedical applications (2012)
Journal Article
Chau, D. Y., Brown, S. V., Mather, M. L., Hutter, V., Tint, N. L., Dua, H. S., …Ghaemmaghami, A. M. (2012). Tissue transglutaminase (TG-2) modified amniotic membrane: A novel scaffold for biomedical applications. Biomedical Materials, 7(4), Article 045011. https://doi.org/10.1088/1748-6041/7/4/045011

The amniotic membrane (AM) is considered as a natural cell culture substrate and has occasionally been exploited in regenerative medicine especially for ocular surface reconstruction and dermal wound healing applications. However, its use is limited... Read More about Tissue transglutaminase (TG-2) modified amniotic membrane: A novel scaffold for biomedical applications.

Uniform cell colonization of porous 3-D scaffolds achieved using radial control of surface chemistry (2011)
Journal Article
Intranuovo, F., Howard, D., White, L. J., Johal, R. K., Ghaemmaghami, A. M., Favia, P., …Alexander, M. R. (2011). Uniform cell colonization of porous 3-D scaffolds achieved using radial control of surface chemistry. Acta Biomaterialia, 7(9), 3336-3344. https://doi.org/10.1016/j.actbio.2011.05.020

Uniform cellular distribution is a prerequisite to forming tissue within porous scaffolds, but the seeding process often results in preferential adhesion of cells at the periphery. We develop a vapour phase coating strategy which is readily applicabl... Read More about Uniform cell colonization of porous 3-D scaffolds achieved using radial control of surface chemistry.