All Outputs (10)

Modelling the impact of wastewater flows and management practices on antimicrobial resistance in dairy farms (2024)
Journal Article
Todman, H., Helliwell, R., King, L., Blanchard, A., Gray-Hammerton, C. J., Hooton, S. P., …Stekel, D. J. (2024). Modelling the impact of wastewater flows and management practices on antimicrobial resistance in dairy farms. npj Antimicrobials & Resistance, 2(1), Article 13. https://doi.org/10.1038/s44259-024-00029-4

Dairy slurry is a major source of environmental contamination with antimicrobial resistant genes and bacteria. We developed mathematical models and conducted on-farm research to explore the impact of wastewater flows and management practices on antim... Read More about Modelling the impact of wastewater flows and management practices on antimicrobial resistance in dairy farms.

Campylobacter bacteriophage DA10: An excised temperate bacteriophage targeted by CRISPR-cas (2020)
Journal Article
Hooton, S., D’Angelantonio, D., Hu, Y., Connerton, P. L., Aprea, G., & Connerton, I. F. (2020). Campylobacter bacteriophage DA10: An excised temperate bacteriophage targeted by CRISPR-cas. BMC Genomics, 21(1), Article 400. https://doi.org/10.1186/s12864-020-06808-3

Background: Lytic bacteriophages that infect Campylobacter spp. have been utilized to develop therapeutic/decontamination techniques. However, the association of Campylobacter spp. and bacteriophages has been the focus of several strands of research... Read More about Campylobacter bacteriophage DA10: An excised temperate bacteriophage targeted by CRISPR-cas.

A generalised model for generalised transduction: the importance of co-evolution and stochasticity in phage mediated antimicrobial resistance transfer (2020)
Journal Article
Arya, S., Todman, H., Baker, M., Hooton, S., Millard, A., Kreft, J. U., …Stekel, D. J. (2020). A generalised model for generalised transduction: the importance of co-evolution and stochasticity in phage mediated antimicrobial resistance transfer. FEMS Microbiology Ecology, 96(7), https://doi.org/10.1093/femsec/fiaa100

© FEMS 2020. Antimicrobial resistance is a major global challenge. Of particular concern are mobilizable elements that can transfer resistance genes between bacteria, leading to pathogens with new combinations of resistance. To date, mathematical mod... Read More about A generalised model for generalised transduction: the importance of co-evolution and stochasticity in phage mediated antimicrobial resistance transfer.

Bacteriophage ZCSE2 is a Potent Antimicrobial against Salmonella enterica Serovars: Ultrastructure, genomics and efficacy (2020)
Journal Article
Mohamed, A., Taha, O., El-Sherif, H. M., Connerton, P. L., Hooton, S. P., Bassim, N. D., …El-Shibiny, A. (2020). Bacteriophage ZCSE2 is a Potent Antimicrobial against Salmonella enterica Serovars: Ultrastructure, genomics and efficacy. Viruses, 12(4), 1-20. https://doi.org/10.3390/v12040424

© 2020 by the authors. Developing novel antimicrobials capable of controlling multidrug-resistant bacterial pathogens is essential to restrict the use of antibiotics. Bacteriophages (phages) constitute a major resource that can be harnessed as an alt... Read More about Bacteriophage ZCSE2 is a Potent Antimicrobial against Salmonella enterica Serovars: Ultrastructure, genomics and efficacy.

Complete Genome Sequences of Vibrio cholerae -Specific Bacteriophages 24 and X29 (2017)
Journal Article
Bhandare, S. G., Warry, A., Emes, R. D., Hooton, S. P., Barrow, P. A., & Atterbury, R. J. (2017). Complete Genome Sequences of Vibrio cholerae -Specific Bacteriophages 24 and X29. Genome Announcements, 5(46), Article e01013. https://doi.org/10.1128/genomeA.01013-17

The complete genomes of two Vibrio cholerae bacteriophages of potential interest for cholera bacteriophage (phage) therapy were sequenced and annotated. The genome size of phage 24 is 44,395 bp encoding 71 putative proteins, and that of phage X29 is... Read More about Complete Genome Sequences of Vibrio cholerae -Specific Bacteriophages 24 and X29.

The bacteriophage carrier state of Campylobacter jejuni features changes in host non-coding RNAs and the acquisition of new host-derived CRISPR spacer sequences (2016)
Journal Article
Hooton, S. P., Brathwaite, K. J., & Connerton, I. F. (2016). The bacteriophage carrier state of Campylobacter jejuni features changes in host non-coding RNAs and the acquisition of new host-derived CRISPR spacer sequences. Frontiers in Microbiology, 7(355), Article 355. https://doi.org/10.3389/fmicb.2016.00355

Incorporation of self-derived CRISPR DNA protospacers in Campylobacter jejuni PT14 occurs in the presence of bacteriophages encoding a CRISPR-like Cas4 protein. This phenomenon was evident in carrier state infections where both bacteriophages and ho... Read More about The bacteriophage carrier state of Campylobacter jejuni features changes in host non-coding RNAs and the acquisition of new host-derived CRISPR spacer sequences.

Campylobacter jejuni acquire new host-derived CRISPR spacers when in association with bacteriophages harboring a CRISPR-like Cas4 protein (2015)
Journal Article
Hooton, S. P., & Connerton, I. F. (2015). Campylobacter jejuni acquire new host-derived CRISPR spacers when in association with bacteriophages harboring a CRISPR-like Cas4 protein. Frontiers in Microbiology, 5(744), https://doi.org/10.3389/fmicb.2014.00744

Campylobacter jejuni is a worldwide cause of human diarrhoeal disease. Clustered Repetitively Interspaced Palindromic Repeats (CRISPRs) and associated proteins allow Bacteria and Archaea to evade bacteriophage and plasmid infection. Type II CRISPR sy... Read More about Campylobacter jejuni acquire new host-derived CRISPR spacers when in association with bacteriophages harboring a CRISPR-like Cas4 protein.

The complete plasmid sequences of Salmonella enterica serovar Typhimurium U288 (2014)
Journal Article
Hooton, S. P., Timms, A. R., Cummings, N. J., Moreton, J., Wilson, R., & Connerton, I. F. (2014). The complete plasmid sequences of Salmonella enterica serovar Typhimurium U288. Plasmid, 76, 32-39. https://doi.org/10.1016/j.plasmid.2014.08.002

Salmonella enterica Serovar Typhimurium U288 is an emerging pathogen of pigs. The strain contains three plasmids of diverse origin that encode traits that are of concern for food security and safety, these include antibiotic resistant determinants, a... Read More about The complete plasmid sequences of Salmonella enterica serovar Typhimurium U288.

Complete genome sequence of Salmonella enterica serovar Typhimurium U288 (2013)
Journal Article
Hooton, S. P., Timms, A. R., Moreton, J., Wilson, R., & Connerton, I. F. (2013). Complete genome sequence of Salmonella enterica serovar Typhimurium U288. Genome Announcements, 1(4), https://doi.org/10.1128/genomeA.00467-13

Salmonella enterica serovar Typhimurium U288 has firmly established itself within the United Kingdom pig production industry. The prevalence of this highly pathogenic multidrug-resistant serovar at such a critical point in the food chain is therefore... Read More about Complete genome sequence of Salmonella enterica serovar Typhimurium U288.

Application of a bacteriophage cocktail to reduce Salmonella Typhimurium U288 contamination on pig skin (2011)
Journal Article
Hooton, S. P., Atterbury, R. J., & Connerton, I. F. (2011). Application of a bacteriophage cocktail to reduce Salmonella Typhimurium U288 contamination on pig skin. International Journal of Food Microbiology, 151(2), https://doi.org/10.1016/j.ijfoodmicro.2011.08.015

Multidrug-resistant Salmonella Typhimurium U288 is a significant pathogen of pigs, accounting for over half of all outbreaks on UK pig production premises. The potential of this serovar, and other salmonellae, to enter the food chain during the slaug... Read More about Application of a bacteriophage cocktail to reduce Salmonella Typhimurium U288 contamination on pig skin.