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Bacteriophage ZCSE2 is a Potent Antimicrobial against Salmonella enterica Serovars: Ultrastructure, genomics and efficacy

Mohamed, Ahmed; Taha, Omar; El-Sherif, Hesham M.; Connerton, Phillippa L.; Hooton, Steven P.T.; Bassim, Nabil D.; Connerton, Ian F.; El-Shibiny, Ayman

Bacteriophage ZCSE2 is a Potent Antimicrobial against Salmonella enterica Serovars: Ultrastructure, genomics and efficacy Thumbnail


Authors

Ahmed Mohamed

Omar Taha

Hesham M. El-Sherif

Phillippa L. Connerton

Steven P.T. Hooton

Nabil D. Bassim

IAN CONNERTON IAN.CONNERTON@NOTTINGHAM.AC.UK
Northern Foods Professor of Food Safety

Ayman El-Shibiny



Abstract

© 2020 by the authors. Developing novel antimicrobials capable of controlling multidrug-resistant bacterial pathogens is essential to restrict the use of antibiotics. Bacteriophages (phages) constitute a major resource that can be harnessed as an alternative to traditional antimicrobial therapies. Phage ZCSE2 was isolated among several others from raw sewage but was distinguished by broad-spectrum activity against Salmonella serovars considered pathogenic to humans and animals. Lytic profiles of ZCSE2 against a panel of Salmonella were determined together with low temperature activity and pH stability. The morphological features of the phage and host infection processes were characterized using a combination of transmission electron and atomic force microscopies. Whole genome sequencing of ZCSE2 produced a complete DNA sequence of 53,965 bp. No known virulence genes were identified in the sequence data, making ZCSE2 a good candidate for phage-mediated biological control purposes. ZCSE2 was further tested against S. Enteritidis in liquid culture and was observed to reduce the target bacterium to below the limits of detection from initial concentrations of 107–108 Colony Forming Units (CFU)/mL. With a broad host-range against pathogenic Salmonella serovars, phage ZCSE2 constitutes a potential tool against a major cause of human and animal disease.

Citation

Mohamed, A., Taha, O., El-Sherif, H. M., Connerton, P. L., Hooton, S. P., Bassim, N. D., …El-Shibiny, A. (2020). Bacteriophage ZCSE2 is a Potent Antimicrobial against Salmonella enterica Serovars: Ultrastructure, genomics and efficacy. Viruses, 12(4), 1-20. https://doi.org/10.3390/v12040424

Journal Article Type Article
Acceptance Date Apr 7, 2020
Online Publication Date Apr 9, 2020
Publication Date Apr 9, 2020
Deposit Date Apr 7, 2020
Publicly Available Date Apr 13, 2020
Journal Viruses
Electronic ISSN 1999-4915
Publisher MDPI
Peer Reviewed Peer Reviewed
Volume 12
Issue 4
Article Number 424
Pages 1-20
DOI https://doi.org/10.3390/v12040424
Keywords bacteriophage ; salmonella ; phage biocontrol ; atomic force microscopy
Public URL https://nottingham-repository.worktribe.com/output/4266278
Publisher URL https://www.mdpi.com/1999-4915/12/4/424

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