All Outputs (16)

Compensating for over-production inhibition of the Hsmar1 transposon in Escherichia coli using a series of constitutive promoters (2020)
Journal Article

© 2020 The Author(s). Background: Transposable elements (TEs) are a diverse group of self-mobilizing DNA elements. Transposition has been exploited as a powerful tool for molecular biology and genomics. However, transposition is sometimes limited bec... Read More about Compensating for over-production inhibition of the Hsmar1 transposon in Escherichia coli using a series of constitutive promoters.

Targeted DNA transposition in vitro using a dCas9-transposase fusion protein (2019)
Journal Article

Homology-directed genome engineering is limited by transgene size. Although DNA transposons are more efficient with large transgenes, random integrations are potentially mutagenic. Here we present an in vitro mechanistic study that demonstrates effic... Read More about Targeted DNA transposition in vitro using a dCas9-transposase fusion protein.

The roles of the human SETMAR (Metnase) protein in illegitimate DNA recombination and non-homologous end joining repair (2019)
Journal Article

SETMAR is a fusion between a SET-domain methyltransferase gene and a mariner-family transposase gene, which is specific to anthropoid primates. However, the ancestral SET gene is present in all other mammals and birds. SETMAR is reported to be involv... Read More about The roles of the human SETMAR (Metnase) protein in illegitimate DNA recombination and non-homologous end joining repair.

Human SETMAR is a DNA sequence-specific histone-methylase with a broad effect on the transcriptome (2018)
Journal Article

Transposons impart dynamism to the genomes they inhabit and their movements frequently rewire the control of nearby genes. Occasionally, their proteins are domesticated when they evolve a new function. SETMAR is a protein methylase with a sequence-sp... Read More about Human SETMAR is a DNA sequence-specific histone-methylase with a broad effect on the transcriptome.

Transposase subunit architecture and its relationship to genome size and the rate of transposition in prokaryotes and eukaryotes (2018)
Journal Article

Cut-and-paste transposons are important tools for mutagenesis, gene-delivery and DNA sequencing applications. At the molecular level, the most thoroughly understood are Tn5 and Tn10 in bacteria, and mariner and hAT elements in eukaryotes. All bacteri... Read More about Transposase subunit architecture and its relationship to genome size and the rate of transposition in prokaryotes and eukaryotes.

A single active site in the mariner transposase cleaves DNA strands of opposite polarity (2017)
Journal Article

The RNase H structural fold defines a large family of nucleic acid metabolizing enzymes that catalyze phosphoryl transfer reactions using two divalent metal ions in the active site. Almost all of these reactions involve only one strand of the nucleic... Read More about A single active site in the mariner transposase cleaves DNA strands of opposite polarity.

The invertebrate lysozyme effector ILYS-3 is systemically activated in response to danger signals and confers antimicrobial protection in C. elegans (2016)
Journal Article

Little is known about the relative contributions and importance of antibacterial effectors in the nematode C. elegans, despite extensive work on the innate immune responses in this organism. We report an investigation of the expression, function and... Read More about The invertebrate lysozyme effector ILYS-3 is systemically activated in response to danger signals and confers antimicrobial protection in C. elegans.

One to rule them all: A highly conserved motif in mariner transposase controls multiple steps of transposition (2014)
Journal Article

The development of transposon-based genome manipulation tools can benefit greatly from understanding transposons’ inherent regulatory mechanisms. The Tc1-mariner transposons, which are being widely used in biotechnological applications, are subject t... Read More about One to rule them all: A highly conserved motif in mariner transposase controls multiple steps of transposition.

Crosstalk between transposase subunits during cleavage of the mariner transposon (2014)
Journal Article

Mariner transposition is a complex reaction that involves three recombination sites and six strand breaking and joining reactions. This requires precise spatial and temporal coordination between the different components to ensure a productive outcome... Read More about Crosstalk between transposase subunits during cleavage of the mariner transposon.

Hyperactive mariner transposons are created by mutations that disrupt allosterism and increase the rate of transposon end synapsis (2013)
Journal Article

New applications for transposons in vertebrate genetics have spurred efforts to develop hyperactive variants. Typically, a genetic screen is used to identify several hyperactive point mutations, which are then incorporated in a single transposase gen... Read More about Hyperactive mariner transposons are created by mutations that disrupt allosterism and increase the rate of transposon end synapsis.

Accuracy and efficiency define Bxb1 integrase as the best of fifteen candidate serine recombinases for the integration of DNA into the human genome (2013)
Journal Article

Background: Phage-encoded serine integrases, such as ?C31 integrase, are widely used for genome engineering. Fifteen such integrases have been described but their utility for genome engineering has not been compared in uniform assays. Results: We h... Read More about Accuracy and efficiency define Bxb1 integrase as the best of fifteen candidate serine recombinases for the integration of DNA into the human genome.

Identification of multiple binding sites for the THAP domain of the Galileo transposase in the long terminal inverted-repeats (2013)
Journal Article

Galileo is a DNA transposon responsible for the generation of several chromosomal inversions in Drosophila. In contrast to other members of the P-element superfamily, it has unusually long terminal inverted-repeats (TIRs) that resemble those of Foldb... Read More about Identification of multiple binding sites for the THAP domain of the Galileo transposase in the long terminal inverted-repeats.

Hsmar1 transposition is sensitive to the topology of the transposon donor and the target (2013)
Journal Article

Hsmar1 is a member of the Tc1-mariner superfamily of DNA transposons. These elements mobilize within the genome of their host by a cut-and-paste mechanism. We have exploited the in vitro reaction provided by Hsmar1 to investigate the effect of DNA su... Read More about Hsmar1 transposition is sensitive to the topology of the transposon donor and the target.

H-NS mediates the dissociation of a refractory protein–DNA complex during Tn10/IS10 transposition (2011)
Journal Article

Tn10/IS10 transposition takes place in the context of a protein–DNA complex called a transpososome. During the reaction, the transpososome undergoes several conformational changes. The host proteins IHF and H-NS, which also are global regulators of g... Read More about H-NS mediates the dissociation of a refractory protein–DNA complex during Tn10/IS10 transposition.

The transposon-like correia elements encode numerous strong promoters and provide a potential new mechanism for phase variation in the meningococcus (2011)
Journal Article

Neisseria meningitidis is the primary causative agent of bacterial meningitis. The genome is rich in repetitive DNA and almost 2% is occupied by a diminutive transposon called the Correia element. Here we report a bioinformatic analysis defining eigh... Read More about The transposon-like correia elements encode numerous strong promoters and provide a potential new mechanism for phase variation in the meningococcus.

Base flipping in Tn10 transposition: an active flip and capture mechanism (2009)
Journal Article

The bacterial Tn5 and Tn10 transposases have a single active site that cuts both strands of DNA at their respective transposon ends. This is achieved using a hairpin intermediate that requires the DNA to change conformation during the reaction. In Tn... Read More about Base flipping in Tn10 transposition: an active flip and capture mechanism.