Azeem Siddique
The transposon-like correia elements encode numerous strong promoters and provide a potential new mechanism for phase variation in the meningococcus
Siddique, Azeem; Buisine, Nicolas; Chalmers, Ronald
Authors
Nicolas Buisine
RONALD CHALMERS RONALD.CHALMERS@NOTTINGHAM.AC.UK
Professor of Biochemistry and Cell Biology
Abstract
Neisseria meningitidis is the primary causative agent of bacterial meningitis. The genome is rich in repetitive DNA and almost 2% is occupied by a diminutive transposon called the Correia element. Here we report a bioinformatic analysis defining eight subtypes of the element with four distinct types of ends. Transcriptional analysis, using PCR and a lacZ reporter system, revealed that two ends in particular encode strong promoters. The activity of the strongest promoter is dictated by a recurrent polymorphism (Y128) at the right end of the element. We highlight examples of elements that appear to drive transcription of adjacent genes and others that may express small non-coding RNAs. Pair-wise comparisons between three meningococcal genomes revealed that no more than two-thirds of Correia elements maintain their subtype at any particular locus. This is due to recombinational class switching between elements in a single strain. Upon switching subtype, a new allele is available to spread through the population by natural transformation. This process may represent a hitherto unrecognized mechanism for phase variation in the meningococcus. We conclude that the strain-to-strain variability of the Correia elements, and the large number of strong promoters encoded by them, allows for potentially widespread effects within the population as a whole. By defining the strength of the promoters encoded by the eight subtypes of Correia ends, we provide a resource that allows the transcriptional effects of a particular subtype at a given locus to be predicted.
Citation
Siddique, A., Buisine, N., & Chalmers, R. (2011). The transposon-like correia elements encode numerous strong promoters and provide a potential new mechanism for phase variation in the meningococcus. PLoS Genetics, 7(1), Article 13. https://doi.org/10.1371/journal.pgen.1001277
Journal Article Type | Article |
---|---|
Publication Date | Jan 20, 2011 |
Deposit Date | Apr 23, 2014 |
Publicly Available Date | Apr 23, 2014 |
Journal | PLoS Genetics |
Print ISSN | 1553-7390 |
Electronic ISSN | 1553-7404 |
Publisher | Public Library of Science |
Peer Reviewed | Peer Reviewed |
Volume | 7 |
Issue | 1 |
Article Number | 13 |
DOI | https://doi.org/10.1371/journal.pgen.1001277 |
Public URL | https://nottingham-repository.worktribe.com/output/707132 |
Publisher URL | http://www.plosgenetics.org/article/info%3Adoi%2F10.1371%2Fjournal.pgen.1001277 |
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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0
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