All Outputs (87)

A genetic assay for gene essentiality in Clostridium (2016)
Journal Article

Essential genes of pathogens are potential therapeutic targets, but are difficult to verify. Here, gene essentiality was determined by targeted knockout following engineered gene duplication. Null mutants of candidate essential genes of Clostridium d... Read More about A genetic assay for gene essentiality in Clostridium.

The binary toxin CDT enhances Clostridium difficile virulence by suppressing protective colonic eosinophilia (2016)
Journal Article

© 2016 Macmillan Publishers Limited. All rights reserved. Clostridium difficile is the most common hospital acquired pathogen in the USA, and infection is, in many cases, fatal. Toxins A and B are its major virulence factors, but expression of a thir... Read More about The binary toxin CDT enhances Clostridium difficile virulence by suppressing protective colonic eosinophilia.

SBRC-Nottingham: sustainable routes to platform chemicals from C1 wastes Gases (2016)
Journal Article

SBRC-Nottingham (www.sbrc-nottingham.ac.uk) was one of the first three UK university-based Synthetic Biology Research Centres (SBRCs) to be funded by the BBSRC and EPSRC as part of the recommendations made in the UKs Synthetic Biology Roadmap. It was... Read More about SBRC-Nottingham: sustainable routes to platform chemicals from C1 wastes Gases.

A roadmap for gene system development in Clostridium (2016)
Journal Article

Clostridium species are both heroes and villains. Some cause serious human and animal diseases, those present in the microbiota contribute to health and wellbeing, while others represent useful industrial chassis for the production of chemicals and f... Read More about A roadmap for gene system development in Clostridium.

Insights into CO2 fixation pathway of Clostridium autoethanogenumby targeted mutagenesis (2016)
Journal Article

The future sustainable production of chemicals and fuels from nonpetrochemical resources and reduction of greenhouse gas emissions are two of the greatest societal challenges. Gas fermentation, which utilizes the ability of acetogenic bacteria such a... Read More about Insights into CO2 fixation pathway of Clostridium autoethanogenumby targeted mutagenesis.

Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824 (2016)
Journal Article

Background: The use of fossil fuels is no longer tenable. Not only are they a finite resource, their use is damaging the environment through pollution and global warming. Alternative, environmentally friendly, renewable sources of chemicals and fuels... Read More about Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824.

CRISPR-based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii (2016)
Journal Article

Solventogenic clostridia are important industrial microorganisms that produce various chemicals and fuels. Effective genetic tools would facilitate physiological studies aimed both at improving our understanding of metabolism and optimizing solvent p... Read More about CRISPR-based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii.

Development of an inducible transposon system for efficient random mutagenesis in Clostridium acetobutylicum (2016)
Journal Article

Clostridium acetobutylicum is an industrially important Gram-positive organism which is capable of producing economically important chemicals in the ABE (Acetone, Butanol and Ethanol) fermentation process. Renewed interests in the ABE process necessi... Read More about Development of an inducible transposon system for efficient random mutagenesis in Clostridium acetobutylicum.

Improving the reproducibility of the NAP1/B1/027 epidemic strain R20291 in the hamster model of infection (2016)
Journal Article

Comparative analysis of the Clostridium difficile BI/NAP1/027 strain R20291 and ClosTron-derived ermB mutants in the hamster infection model are compromised by the clindamycin susceptibility of the parent. Mutants can appear more virulent. We have re... Read More about Improving the reproducibility of the NAP1/B1/027 epidemic strain R20291 in the hamster model of infection.

Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824 (2016)
Journal Article

Background Clostridium acetobutylicum represents a paradigm chassis for the industrial production of the biofuel biobutanol and a focus for metabolic engineering. We have previously developed procedures for the creation of in-frame, marker-less de... Read More about Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824.

Whole genome sequence and manual annotation of Clostridium autoethanogenum, an industrially relevant bacterium (2015)
Journal Article

© 2015 Humphreys et al. Background: Clostridium autoethanogenum is an acetogenic bacterium capable of producing high value commodity chemicals and biofuels from the C1 gases present in synthesis gas. This common industrial waste gas can act as the so... Read More about Whole genome sequence and manual annotation of Clostridium autoethanogenum, an industrially relevant bacterium.

Clostridium difficile-mediated effects on human intestinal epithelia: Modelling host-pathogen interactions in a vertical diffusion chamber (2015)
Journal Article

Clostridium difficile infection is one of the leading causes of healthcare associated diarrhoea in the developed world. Although the contribution of C. difficile toxins to disease pathogenesis is now well understood, many facets of host-pathogen inte... Read More about Clostridium difficile-mediated effects on human intestinal epithelia: Modelling host-pathogen interactions in a vertical diffusion chamber.

A universal mariner transposon system for forward genetic studies in the genus Clostridium (2015)
Journal Article

DNA transposons represent an essential tool in the armoury of the molecular microbiologist. We previously developed a catP-based mini transposon system for Clostridium difficile in which the expression of the transposase gene was dependent on a sigma... Read More about A universal mariner transposon system for forward genetic studies in the genus Clostridium.

Coenzyme A-transferase-independent butyrate re-assimilation in Clostridium acetobutylicum - evidence from a mathematical model (2014)
Journal Article

The hetero-dimeric CoA-transferase CtfA/B is believed to be crucial for the metabolic transition from acidogenesis to solventogenesis in Clostridium acetobutylicum as part of the industrial-relevant acetone-butanol-ethanol (ABE) fermentation. Here, t... Read More about Coenzyme A-transferase-independent butyrate re-assimilation in Clostridium acetobutylicum - evidence from a mathematical model.

The role of flagella in Clostridium difficile pathogenesis:comparison between a non-epidemic and an epidemicstrain (2013)
Journal Article

Clostridium difficile is a major cause of healthcare-associated infection and inflicts a considerable financial burden on healthcare systems worldwide. Disease symptoms range from self-limiting diarrhoea to fatal pseudomembranous colitis. Whilst C. d... Read More about The role of flagella in Clostridium difficile pathogenesis:comparison between a non-epidemic and an epidemicstrain.

Secretion and assembly of functional mini-cellulosomes from synthetic chromosomal operons in Clostridium acetobutylicum ATCC 824 (2013)
Journal Article

BACKGROUND: Consolidated bioprocessing (CBP) is reliant on the simultaneous enzyme production, saccharification of biomass, and fermentation of released sugars into valuable products such as butanol. Clostridial species that produce butanol are, h... Read More about Secretion and assembly of functional mini-cellulosomes from synthetic chromosomal operons in Clostridium acetobutylicum ATCC 824.

Importance of Toxin A, Toxin B, and CDT in virulence of an epidemic Clostridium difficile strain (2013)
Journal Article

Clostridium difficile infection is the main cause of healthcare-acquired diarrhea in the developed world. In addition to the main virulence factors toxin A and B, epidemic, PCR Ribotype 027 strains, such as R20291, produce a third toxin, CDT. To deve... Read More about Importance of Toxin A, Toxin B, and CDT in virulence of an epidemic Clostridium difficile strain.

Clostridium difficile modulates host innate immunity via toxin-independent and dependent mechanism(s) (2013)
Journal Article

Clostridium difficile infection (CDI) is the leading cause of hospital and community-acquired antibiotic-associated diarrhoea and currently represents a significant health burden. Although the role and contribution of C. difficile toxins to disease p... Read More about Clostridium difficile modulates host innate immunity via toxin-independent and dependent mechanism(s).

The putative influence of the agr operon upon survival mechanisms used by Clostridium acetobutylicum (2013)
Journal Article

The bacterium Clostridium acetobutylicum produces acids as an energy-yielding process during exponential growth. An acidic environment, however, is toxic to the cells and two survival mechanisms are in place to prevent them from dying. Firstly, durin... Read More about The putative influence of the agr operon upon survival mechanisms used by Clostridium acetobutylicum.

Expanding the repertoire of gene tools for precise manipulation of the Clostridium difficile genome: allelic exchange using pyrE alleles (2013)
Journal Article

Sophisticated genetic tools to modify essential biological processes at the molecular level are pivotal in elucidating the molecular pathogenesis of Clostridium difficile, a major cause of healthcare associated disease. Here we have developed an effi... Read More about Expanding the repertoire of gene tools for precise manipulation of the Clostridium difficile genome: allelic exchange using pyrE alleles.