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Importance of Toxin A, Toxin B, and CDT in virulence of an epidemic Clostridium difficile strain

Kuehne, Sarah A.; Collery, Mark M.; Kelly, Michelle L.; Cartman, Stephen T.; Cockayne, Alan; Minton, Nigel P.

Authors

Sarah A. Kuehne sarah.keuhne@nottingham.av.uk

Mark M. Collery

Michelle L. Kelly

Stephen T. Cartman

Alan Cockayne

Nigel P. Minton nigel.minton@nottingham.ac.uk

Abstract

Clostridium difficile infection is the main cause of healthcare-acquired diarrhea in the developed world. In addition to the main virulence factors toxin A and B, epidemic, PCR Ribotype 027 strains, such as R20291, produce a third toxin, CDT. To develop effective medical countermeasures, it is important to understand the importance of each toxin. Accordingly, we created all possible combinations of isogenic toxin mutants of R20291 and assessed their virulence. We demonstrated that either toxin A or toxin B alone can cause fulminant disease in the hamster infection model and present tantalizing data that C. difficile toxin may also contribute to virulence.

Journal Article Type Article
Journal Journal of Infectious Diseases
Print ISSN 0022-1899
Electronic ISSN 1537-6613
Publisher Oxford University Press (OUP)
Peer Reviewed Peer Reviewed
Volume 209
Issue 1
Institution Citation Kuehne, S. A., Collery, M. M., Kelly, M. L., Cartman, S. T., Cockayne, A., & Minton, N. P. (in press). Importance of Toxin A, Toxin B, and CDT in virulence of an epidemic Clostridium difficile strain. Journal of Infectious Diseases, 209(1), doi:10.1093/infdis/jit426
DOI https://doi.org/10.1093/infdis/jit426
Keywords Clostridium difficile infection; TcdA; TcdB; pathogenesis; CDT.
Publisher URL http://jid.oxfordjournals.org/content/209/1/83
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0




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