Improving the reproducibility of the NAP1/B1/027 epidemic strain R20291 in the hamster model of infection

Kelly, Michelle L.; Ng, Yen Kuang; Cartman, Stephen T.; Collery, Mark M.; Cockayne, Alan; Minton, Nigel P.

Abstract

Comparative analysis of the Clostridium difficile BI/NAP1/027 strain R20291 and ClosTron-derived ermB mutants in the hamster infection model are compromised by the clindamycin susceptibility of the parent. Mutants can appear more virulent. We have rectified this anomaly by genome engineering. The variant created (CRG20291) represents an ideal control strain for virulence assays of ClosTron mutants.

Citation

Kelly, M. L., Ng, Y. K., Cartman, S. T., Collery, M. M., Cockayne, A., & Minton, N. P. (2016). Improving the reproducibility of the NAP1/B1/027 epidemic strain R20291 in the hamster model of infection. Anaerobe, 39, 51-53. https://doi.org/10.1016/j.anaerobe.2016.02.011

• Journal Article Type: Article
• Acceptance Date: Feb 23, 2016
• Online Publication Date: Mar 2, 2016
• Publication Date: Jun 1, 2016
• Deposit Date: Sep 28, 2017
• Publicly Available Date: May 13, 2019
• Print ISSN: 1075-9964
• Electronic ISSN: 1095-8274
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 39
• Pages: 51-53
• DOI: https://doi.org/10.1016/j.anaerobe.2016.02.011
• Keywords: Clostridium difficile; Hamster model; Clindamycin sensitivity; ermB; Genome engineering
• Public URL: https://nottingham-repository.worktribe.com/output/1112204
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S1075996416300142?via%3Dihub
• PMID: 26946361