All Outputs (11)

Characterization of the impact of rpoB mutations on the in vitro and in vivo competitive fitness of Clostridium difficile and susceptibility to fidaxomicin (2017)
Journal Article

Objectives To establish the role of specific, non-synonymous SNPs in the RNA polymerase ? subunit (rpoB) gene in reducing the susceptibility of Clostridium difficile to fidaxomicin and to explore the potential in vivo significance of rpoB mutant str... Read More about Characterization of the impact of rpoB mutations on the in vitro and in vivo competitive fitness of Clostridium difficile and susceptibility to fidaxomicin.

13C-assisted metabolic flux analysis to investigate heterotrophic and mixotrophic metabolism in Cupriavidus necator H16 (2017)
Journal Article

Introduction. Cupriavidus necator H16 is a gram-negative bacterium, capable of lithoautotrophic growth by utilizing hydrogen as an energy source and fixing carbon dioxide (CO2) through Calvin-Benson-Bassham (CBB) cycle. The potential to utilize synth... Read More about 13C-assisted metabolic flux analysis to investigate heterotrophic and mixotrophic metabolism in Cupriavidus necator H16.

Applying asymptotic methods to synthetic biology: modelling the reaction kinetics of the mevalonate pathway (2017)
Journal Article

The mevalonate pathway is normally found in eukaryotes, and allows for the production of isoprenoids, a useful class of organic compounds. This pathway has been successfully introduced to Escherichia coli, enabling a biosynthetic production route for... Read More about Applying asymptotic methods to synthetic biology: modelling the reaction kinetics of the mevalonate pathway.

Multi-timescale analysis of a metabolic network in synthetic biology: a kinetic model for 3-hydroxypropionic acid production via beta-alanine (2017)
Journal Article

A biosustainable production route for 3-hydroxypropionic acid (3HP), an important platform chemical, would allow 3HP to be produced without using fossil fuels. We are interested in investigating a potential biochemical route to 3HP from pyruvate thro... Read More about Multi-timescale analysis of a metabolic network in synthetic biology: a kinetic model for 3-hydroxypropionic acid production via beta-alanine.

Recombinant expression and characterisation of the oxygen-sensitive 2-enoate reductase from Clostridium sporogenes (2017)
Journal Article

‘Ene’-reductases have attracted significant attention for the preparation of chemical intermediates and biologically active products. To date, research has been focussed primarily on Old Yellow Enzyme-like proteins, due to their ease of handling, whe... Read More about Recombinant expression and characterisation of the oxygen-sensitive 2-enoate reductase from Clostridium sporogenes.

Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants (2017)
Journal Article

Effective microbial metabolic engineering is reliant on efficient gene transfer. Here we present a simple screening strategy that may be deployed to isolate rare, hypertransformable variants. The procedure was used to increase the frequency of transf... Read More about Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants.

Characterisation of a 3-hydroxypropionic acid-inducible system from Pseudomonas putida for orthogonal gene expression control in Escherichia coli and Cupriavidus necator (2017)
Journal Article

3-hydroxypropionic acid (3-HP) is an important platform chemical used as a precursor for production of added-value compounds such as acrylic acid. Metabolically engineered yeast, Escherichia coli, cyanobacteria and other microorganisms have been deve... Read More about Characterisation of a 3-hydroxypropionic acid-inducible system from Pseudomonas putida for orthogonal gene expression control in Escherichia coli and Cupriavidus necator.

Microbial solvent formation revisited by comparative genome analysis (2017)
Journal Article

Background: Microbial formation of acetone, isopropanol, and butanol is largely restricted to bacteria belonging to the genus Clostridium. This ability has been industrially exploited over the last 100 years. The solvents are important feedstocks for... Read More about Microbial solvent formation revisited by comparative genome analysis.

Towards improved butanol production through targeted genetic modification of Clostridium pasteurianum (2017)
Journal Article

Declining fossil fuel reserves, coupled with environmental concerns over their continued extraction and exploitation have led to strenuous efforts to identify renewable routes to energy and fuels. One attractive option is to convert glycerol, a by-pr... Read More about Towards improved butanol production through targeted genetic modification of Clostridium pasteurianum.

Metabolic engineering of Clostridium autoethanogenum for selective alcohol production (2017)
Journal Article

Gas fermentation using acetogenic bacteria such as Clostridium autoethanogenum offers an attractive route for production of fuel ethanol from industrial waste gases. Acetate reduction to acetaldehyde and further to ethanol via an aldehyde: ferredoxin... Read More about Metabolic engineering of Clostridium autoethanogenum for selective alcohol production.

Development of Clostridium difficile R20291?PaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels (2017)
Journal Article

Assessing the regulation of Clostridium difficile transferase (CDT), is complicated by the presence of a Pathogenicity locus (PaLoc) which encodes Toxins A and B. Here we developed R20291?PaLoc model strains and cell-based assays to quantify CDT-medi... Read More about Development of Clostridium difficile R20291?PaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels.