Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants

Grosse-Honebrink, Alexander; Schwarz, Katrin; Wang, Hengzheng; Minton, Nigel P.; Zhang, Ying

Abstract

Effective microbial metabolic engineering is reliant on efficient gene transfer. Here we present a simple screening strategy that may be deployed to isolate rare, hypertransformable variants. The procedure was used to increase the frequency of transformation of the solvent producing organism Clostridium pasteurianum by three to four orders of magnitude.

Citation

Grosse-Honebrink, A., Schwarz, K., Wang, H., Minton, N. P., & Zhang, Y. (2017). Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants. Anaerobe, 48, https://doi.org/10.1016/j.anaerobe.2017.09.001

• Journal Article Type: Article
• Acceptance Date: Sep 5, 2017
• Online Publication Date: Sep 9, 2017
• Publication Date: Dec 31, 2017
• Deposit Date: Oct 20, 2017
• Publicly Available Date: Oct 20, 2017
• Journal: Anaerobe
• Print ISSN: 1075-9964
• Electronic ISSN: 1095-8274
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 48
• DOI: https://doi.org/10.1016/j.anaerobe.2017.09.001
• Keywords: Clostridium pasteurianum; DNA transfer/transformation efficiency; Structural maintenance of chromosomes (SMC)
• Public URL: https://nottingham-repository.worktribe.com/output/902534
• Publisher URL: https://doi.org/10.1016/j.anaerobe.2017.09.001
• Contract Date: Oct 20, 2017