Alexander Grosse-Honebrink
Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants
Grosse-Honebrink, Alexander; Schwarz, Katrin; Wang, Hengzheng; Minton, Nigel P.; Zhang, Ying
Authors
Katrin Schwarz
Hengzheng Wang
Professor NIGEL MINTON nigel.minton@nottingham.ac.uk
Professor of Applied Molecular Microbiology
YING ZHANG YING.ZHANG@NOTTINGHAM.AC.UK
Assistant Professor
Abstract
Effective microbial metabolic engineering is reliant on efficient gene transfer. Here we present a simple screening strategy that may be deployed to isolate rare, hypertransformable variants. The procedure was used to increase the frequency of transformation of the solvent producing organism Clostridium pasteurianum by three to four orders of magnitude.
Citation
Grosse-Honebrink, A., Schwarz, K., Wang, H., Minton, N. P., & Zhang, Y. (2017). Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants. Anaerobe, 48, https://doi.org/10.1016/j.anaerobe.2017.09.001
Journal Article Type | Article |
---|---|
Acceptance Date | Sep 5, 2017 |
Online Publication Date | Sep 9, 2017 |
Publication Date | Dec 31, 2017 |
Deposit Date | Oct 20, 2017 |
Publicly Available Date | Oct 20, 2017 |
Journal | Anaerobe |
Print ISSN | 1075-9964 |
Electronic ISSN | 1095-8274 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 48 |
DOI | https://doi.org/10.1016/j.anaerobe.2017.09.001 |
Keywords | Clostridium pasteurianum; DNA transfer/transformation efficiency; Structural maintenance of chromosomes (SMC) |
Public URL | https://nottingham-repository.worktribe.com/output/902534 |
Publisher URL | https://doi.org/10.1016/j.anaerobe.2017.09.001 |
Contract Date | Oct 20, 2017 |
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Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0
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