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13C-assisted metabolic flux analysis to investigate heterotrophic and mixotrophic metabolism in Cupriavidus necator H16

Alagesan, Swathi; Minton, Nigel P.; Malys, Naglis

13C-assisted metabolic flux analysis to investigate heterotrophic and mixotrophic metabolism in Cupriavidus necator H16 Thumbnail


Authors

Swathi Alagesan

Naglis Malys



Abstract

Introduction. Cupriavidus necator H16 is a gram-negative bacterium, capable of lithoautotrophic growth by utilizing hydrogen as an energy source and fixing carbon dioxide (CO2) through Calvin-Benson-Bassham (CBB) cycle. The potential to utilize synthesis gas (Syngas) and the prospects of rerouting carbon from polyhydroxybutyrate synthesis to value-added compounds makes C. necator an excellent chassis for industrial application.
Objectives. In the context of lack of sufficient quantitative information of the metabolic pathways and to advance in rational metabolic engineering for optimized product synthesis in C. necator H16, we carried out a metabolic flux analysis based on steady-state 13C-labelling.
Methods. In this study, steady-state carbon labelling experiments, using either D-[1-13C]fructose or [1,2-13C]glycerol, were undertaken to investigate the carbon flux through the central carbon metabolism in C. necator H16 under heterotrophic and mixotrophic growth conditions, respectively.
Results. We found that the CBB cycle is active even under heterotrophic condition, and growth is indeed mixotrophic. While Entner-Doudoroff (ED) pathway is shown to be the major route for sugar degradation, tricarboxylic acid (TCA) cycle is highly active in mixotrophic condition. Enhanced flux is observed in reductive pentose phosphate pathway (redPPP) under the mixotrophic condition to supplement the precursor requirement for CBB cycle. The flux distribution was compared to the mRNA abundance of genes encoding enzymes involved in key enzymatic reactions of the central carbon metabolism.
Conclusion. This study leads the way to establishing 13C-based quantitative fluxomics for rational pathway engineering in C. necator H16.

Citation

Alagesan, S., Minton, N. P., & Malys, N. (2018). 13C-assisted metabolic flux analysis to investigate heterotrophic and mixotrophic metabolism in Cupriavidus necator H16. Metabolomics, 14(9), https://doi.org/10.1007/s11306-017-1302-z

Journal Article Type Article
Acceptance Date Nov 17, 2017
Online Publication Date Dec 4, 2017
Publication Date Jan 1, 2018
Deposit Date Dec 5, 2017
Publicly Available Date Mar 29, 2024
Journal Metabolomics
Electronic ISSN 1573-3890
Publisher Springer Verlag
Peer Reviewed Peer Reviewed
Volume 14
Issue 9
DOI https://doi.org/10.1007/s11306-017-1302-z
Keywords Cupriavidus necator H16, metabolic flux analysis, 13C-labelling, steady-state, amino acids, RT-PCR
Public URL https://nottingham-repository.worktribe.com/output/963096
Publisher URL https://link.springer.com/article/10.1007%2Fs11306-017-1302-z

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