A novel D2O tracer method to quantify RNA turnover as a biomarker of de novo ribosomal biogenesis, in vitro, in animal models, and in human skeletal muscle

Brook, Matthew S.; Wilkinson, D.J.; Mitchell, W. Kyle; Lund, Jonathan N.; Phillips, Bethan E.; Szewczyk, Nathaniel J.; Kainulainen, H.; Lensu, S.; Koch, L.G.; Britton, S.L.; Greenhaff, Paul L.; Smith, K.; Atherton, Philip J.

doi:10.1152/ajpendo.00157.2017

All Outputs (3)

Recent developments in deuterium oxide tracer approaches to measure rates of substrate turnover: implications for protein, lipid, and nucleic acid research (2017)
Journal Article

Purpose of review: Methods that inform on dynamic metabolism that can be applied to clinical populations to understand disease progression and responses to therapeutic interventions are of great importance. This review perspective will highlight rece... Read More about Recent developments in deuterium oxide tracer approaches to measure rates of substrate turnover: implications for protein, lipid, and nucleic acid research.

Nutrient modulation in the management of disease-induced muscle wasting: evidence from human studies (2017)
Journal Article

Purpose of review: In addition to being essential for movement, skeletal muscles act as both a store and source of key macronutrients. As such, muscle is an important tissue for whole body homeostasis, undergoing muscle wasting in times of starvation... Read More about Nutrient modulation in the management of disease-induced muscle wasting: evidence from human studies.

A novel D2O tracer method to quantify RNA turnover as a biomarker of de novo ribosomal biogenesis, in vitro, in animal models, and in human skeletal muscle (2017)
Journal Article

Current methods to quantify in vivo RNA dynamics are limited. Here, we developed a novel stable isotope (D2O) methodology to quantify RNA synthesis (i.e., ribosomal biogenesis) in cells, animal models, and humans. First, proliferating C2C12 cells wer... Read More about A novel D2O tracer method to quantify RNA turnover as a biomarker of de novo ribosomal biogenesis, in vitro, in animal models, and in human skeletal muscle.