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Recent developments in deuterium oxide tracer approaches to measure rates of substrate turnover: implications for protein, lipid, and nucleic acid research

Brook, Matthew S.; Wilkinson, Daniel J.; Atherton, Philip J.; Smith, Kenneth

Authors

Matthew S. Brook

Daniel J. Wilkinson

Philip J. Atherton

Kenneth Smith



Abstract

Purpose of review: Methods that inform on dynamic metabolism that can be applied to clinical populations to understand disease progression and responses to therapeutic interventions are of great importance. This review perspective will highlight recent advances, development, and applications of the multivalent stable isotope tracer deuterium oxide (D2O) to the study of substrate metabolism with particular reference to protein, lipids, and nucleic acids, and how these methods can be readily applied within clinical and pharmaceutical research.

Recent findings: Advances in the application of D2O techniques now permit the simultaneous dynamic measurement of a range of substrates (i.e. protein, lipid, and nucleic acids, along with the potential for OMICs methodologies) with minimal invasiveness further creating opportunities for long-term ‘free living’ measures that can be used in clinical settings. These techniques have recently been applied to ageing populations and further in cancer patients revealing altered muscle protein metabolism. Additionally, the efficacy of numerous drugs in improving lipoprotein profiles and controlling cellular proliferation in leukaemia have been revealed.

Summary: D2O provides opportunities to create a more holistic picture of in-vivo metabolic phenotypes, providing a unique platform for development in clinical applications, and the emerging field of personalized medicine.

Journal Article Type Article
Publication Date Sep 1, 2017
Journal Current Opinion in Clinical Nutrition and Metabolic Care
Print ISSN 1363-1950
Electronic ISSN 1473-6519
Publisher Lippincott, Williams & Wilkins
Peer Reviewed Peer Reviewed
Volume 20
Issue 5
APA6 Citation Brook, M. S., Wilkinson, D. J., Atherton, P. J., & Smith, K. (2017). Recent developments in deuterium oxide tracer approaches to measure rates of substrate turnover: implications for protein, lipid, and nucleic acid research. Current Opinion in Clinical Nutrition and Metabolic Care, 20(5), doi:10.1097/MCO.0000000000000392
DOI https://doi.org/10.1097/MCO.0000000000000392
Publisher URL https://insights.ovid.com/crossref?an=00075197-201709000-00011
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc/4.0





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