All Outputs (29)

Sensitive recovery of recombinant antibody clones after their in silico identification within NGS datasets (2015)
Journal Article
Spiliotopoulos, A., Owen, J. P., Maddison, B. C., Dreveny, I., Rees, H. C., & Gough, K. C. (2015). Sensitive recovery of recombinant antibody clones after their in silico identification within NGS datasets. Journal of Immunological Methods, 420, https://doi.org/10.1016/j.jim.2015.03.005

Recently the analytical power of the latest high throughput next generation DNA sequencing platforms has been used to analyse phage that have been selected from the panning of large combinatorial libraries displaying either peptide or antibody ligand... Read More about Sensitive recovery of recombinant antibody clones after their in silico identification within NGS datasets.

Incubation of ovine scrapie with environmental matrix results in biological and biochemical changes of PrPSc over time (2015)
Journal Article
Maddison, B. C., Spiropoulos, J., Vickery, C. M., Lockey, R., Owen, J. P., Bishop, K., …Gough, K. C. (2015). Incubation of ovine scrapie with environmental matrix results in biological and biochemical changes of PrPSc over time. Veterinary Research, 46, Article 46. https://doi.org/10.1186/s13567-015-0179-y

Ovine scrapie can be transmitted via environmental reservoirs. A pool of ovine scrapie isolates were incubated on soil for one day or thirteen months and eluted prion was used to challenge tg338 mice transgenic for ovine PrP. After one-day incubation... Read More about Incubation of ovine scrapie with environmental matrix results in biological and biochemical changes of PrPSc over time.

Circulation of prions within dust on a scrapie affected farm (2015)
Journal Article
Gough, K. C., Baker, C. A., Simmons, H. A., Hawkins, S. A., & Maddison, B. C. (2015). Circulation of prions within dust on a scrapie affected farm. Veterinary Research, 46, https://doi.org/10.1186/s13567-015-0176-1

Prion diseases are fatal neurological disorders that affect humans and animals. Scrapie of sheep/goats and Chronic Wasting Disease (CWD) of deer/elk are contagious prion diseases where environmental reservoirs have a direct link to the transmission o... Read More about Circulation of prions within dust on a scrapie affected farm.

Persistence of scrapie infectivity within a farm environment after cleaning and decontamination (2015)
Journal Article
Hawkins, S. A., Simmons, H. A., Gough, K. C., & Maddison, B. C. (2015). Persistence of scrapie infectivity within a farm environment after cleaning and decontamination. Veterinary Record, 176(4), https://doi.org/10.1136/vr.102743

Scrapie of sheep/goats and Chronic Wasting Disease (CWD) of deer/elk are contagious prion diseases where environmental reservoirs are directly implicated in the transmission of disease. In this study the effectiveness of recommended scrapie farm deco... Read More about Persistence of scrapie infectivity within a farm environment after cleaning and decontamination.

Prebiotic and probiotic agents enhance antibody-based immune responses to Salmonella Typhimurium infection in pigs (2014)
Journal Article
Naqid, I. A., Owen, J. P., Maddison, B. C., Gardner, D. S., Foster, N., Tchorzewska, M., La Ragione, R. M., & Gough, K. C. (2015). Prebiotic and probiotic agents enhance antibody-based immune responses to Salmonella Typhimurium infection in pigs. Animal Feed Science and Technology, 201, 57-65. https://doi.org/10.1016/j.anifeedsci.2014.12.005

Salmonellosis causes significant economic losses to the pig industry and contaminated pork products are an important source of Salmonella for humans. The EU ban on the use of antibiotic growth promoters in pig production, and the emergence of antibio... Read More about Prebiotic and probiotic agents enhance antibody-based immune responses to Salmonella Typhimurium infection in pigs.

The application of eDNA for monitoring of the great crested newt in the UK (2014)
Journal Article
Rees, H. C., Bishop, K., Middleditch, D. J., Patmore, J. R., Maddison, B. C., & Gough, K. C. (2014). The application of eDNA for monitoring of the great crested newt in the UK. Ecology and Evolution, 4(21), https://doi.org/10.1002/ece3.1272

Current ecological surveys for great crested newts are time-consuming and expensive and can only be carried out within a short survey window. Additional survey methods which would facilitate the detection of rare or protected species such as the grea... Read More about The application of eDNA for monitoring of the great crested newt in the UK.

Highly sensitive detection of small ruminant bovine spongiform encephalopathy within transmissible spongiform encephalopathy mixes by serial protein misfolding cyclic amplification (2014)
Journal Article
Gough, K. C., Bishop, K., & Maddison, B. C. (2014). Highly sensitive detection of small ruminant bovine spongiform encephalopathy within transmissible spongiform encephalopathy mixes by serial protein misfolding cyclic amplification. Journal of Clinical Microbiology, 52(11), https://doi.org/10.1128/JCM.01693-14

It is assumed that sheep and goats consumed the same bovine spongiform encephalopathy (BSE)-contaminated meat and bone meal that was fed to cattle and precipitated the BSE epidemic in the United Kingdom that peaked more than 20 years ago. De- spite i... Read More about Highly sensitive detection of small ruminant bovine spongiform encephalopathy within transmissible spongiform encephalopathy mixes by serial protein misfolding cyclic amplification.

The detection of aquatic animal species using environmental DNA – a review of eDNA as a survey tool in ecology (2014)
Journal Article
Rees, H. C., Maddison, B. C., Middleditch, D. J., Patmore, J. R., & Gough, K. C. (2014). The detection of aquatic animal species using environmental DNA – a review of eDNA as a survey tool in ecology. Journal of Applied Ecology, 51, https://doi.org/10.1111/1365-2664.12306

1. Knowledge of species distribution is critical to ecological management and conservation biology. Effective management requires the detection of populations, which can sometimes be at low densities and is usually based on visual detection and count... Read More about The detection of aquatic animal species using environmental DNA – a review of eDNA as a survey tool in ecology.

Recombinant plants provide a new approach to the production of bacterial polysaccharide for vaccines (2014)
Journal Article
Smith, C. M., Fry, S. C., Gough, K. C., Patel, A. J., Glenn, S., Goldrick, M., …Andrew, P. W. (2014). Recombinant plants provide a new approach to the production of bacterial polysaccharide for vaccines. PLoS ONE, 9(2), Article e88144. https://doi.org/10.1371/journal.pone.0088144

Bacterial polysaccharides have numerous clinical or industrial uses. Recombinant plants could offer the possibility of producing bacterial polysaccharides on a large scale and free of contaminating bacterial toxins and antigens. We investigated the f... Read More about Recombinant plants provide a new approach to the production of bacterial polysaccharide for vaccines.