All Outputs (78)

Protein kinase CK2 inactivates PRH/Hhex using multiple mechanisms to de-repress VEGF-signalling genes and promote cell survival (2012)
Journal Article
Noy, P., Sawasdichai, A., Jayaraman, P., & Gaston, K. (2012). Protein kinase CK2 inactivates PRH/Hhex using multiple mechanisms to de-repress VEGF-signalling genes and promote cell survival. Nucleic Acids Research, 40(18), 9008--9020. https://doi.org/10.1093/nar/gks687

Protein kinase CK2 promotes cell survival and the activity of this kinase is elevated in several cancers including chronic myeloid leukaemia. We have shown previously that phosphorylation of the Proline-Rich Homeodomain protein (PRH/Hhex) by CK2 inhi... Read More about Protein kinase CK2 inactivates PRH/Hhex using multiple mechanisms to de-repress VEGF-signalling genes and promote cell survival.

The proline rich homeodomain protein PRH/Hhex forms stable oligomers that are highly resistant to denaturation (2012)
Journal Article
Shukla, A., Burton, N. M., Jayaraman, P., & Gaston, K. (2012). The proline rich homeodomain protein PRH/Hhex forms stable oligomers that are highly resistant to denaturation. PLoS ONE, 7(4), Article e35984. https://doi.org/10.1371/journal.pone.0035984

Background: Many transcription factors control gene expression by binding to specific DNA sequences at or near the genes that they regulate. However, some transcription factors play more global roles in the control of gene expression by altering the... Read More about The proline rich homeodomain protein PRH/Hhex forms stable oligomers that are highly resistant to denaturation.

Small DNA tumour viruses (2012)
Book
(2012). K. Gaston (Ed.), Small DNA tumour viruses. Wymondham: Caister Academic Press

Protein flexibility directs DNA recognition by the papillomavirus E2 proteins (2010)
Journal Article
Brown, C. A., Campos-León, K., Strickland, M., Williams, C., Fairweather, V., Brady, R. L., …Gaston, K. (2011). Protein flexibility directs DNA recognition by the papillomavirus E2 proteins. Nucleic Acids Research, 39(7), 2969--2980. https://doi.org/10.1093/nar/gkq1217

Although DNA flexibility is known to play an important role in DNA–protein interactions, the importance of protein flexibility is less well understood. Here, we show that protein dynamics are important in DNA recognition using the well-characterized... Read More about Protein flexibility directs DNA recognition by the papillomavirus E2 proteins.

DNA compaction by the higher-order assembly of PRH/Hex homeodomain protein oligomers (2010)
Journal Article
Soufi, A., Sawasdichai, A., Shukla, A., Noy, P., Dafforn, T., Smith, C., …Gaston, K. (2010). DNA compaction by the higher-order assembly of PRH/Hex homeodomain protein oligomers. Nucleic Acids Research, 38(21), 7513--7525. https://doi.org/10.1093/nar/gkq659

Protein self-organization is essential for the establishment and maintenance of nuclear architecture and for the regulation of gene expression. We have shown previously that the Proline-Rich Homeodomain protein (PRH/Hex) self-assembles to form oligom... Read More about DNA compaction by the higher-order assembly of PRH/Hex homeodomain protein oligomers.

In situ subcellular fractionation of adherent and non-adherent mammalian cells (2010)
Journal Article
Sawasdichai, A., Chen, H., Abdul Hamid, N., Jayaraman, P., & Gaston, K. (2010). In situ subcellular fractionation of adherent and non-adherent mammalian cells. Journal of Visualized Experiments, 41, Article e1958. https://doi.org/10.3791/1958

Protein function is intimately coupled to protein localization. Although some proteins are restricted to a specific location or subcellular compartment, many proteins are present as a freely diffusing population in free exchange with a sub-population... Read More about In situ subcellular fractionation of adherent and non-adherent mammalian cells.

PRH/Hex controls cell survival through coordinate transcriptional regulation of vascular endothelial growth factor signaling (2010)
Journal Article
Noy, P., Williams, H., Sawasdichai, A., Gaston, K., & Jayaraman, P. (2010). PRH/Hex controls cell survival through coordinate transcriptional regulation of vascular endothelial growth factor signaling. Molecular and Cellular Biology, 30(9), 2120-2134. doi:10.1128/MCB.01511-09

The proline-rich homeodomain protein (PRH) plays multiple roles in the control of gene expression during embryonic development and in the adult. Vascular endothelial growth factor (VEGF) is a mitogen that stimulates cell proliferation and survival vi... Read More about PRH/Hex controls cell survival through coordinate transcriptional regulation of vascular endothelial growth factor signaling.

The silver lining: the importance of higher qualifications in a global recession (2009)
Other
Gaston, K. (2009). The silver lining: the importance of higher qualifications in a global recession

The human papillomavirus E7-E2 interaction mechanism in vitro reveals a finely turned system for modulating available E7 and E2 proteins (2009)
Journal Article
Smal, C., Wetzler, D. E., Dantur, K. I., Chemes, L. B., Garcia-Alai, M. M., Dellarole, M., …de Prat Gay, G. (2009). The human papillomavirus E7-E2 interaction mechanism in vitro reveals a finely turned system for modulating available E7 and E2 proteins. Biochemistry, 48(50), 11939-11949. https://doi.org/10.1021/bi901415k

Transcription of the human papillomavirus E7 oncoprotein is negatively controlled by the viral E2 protein, and loss of this repression leads to irreversible transformation and carcinogenesis. Here we show that interaction of the HPV16 E7 protein with... Read More about The human papillomavirus E7-E2 interaction mechanism in vitro reveals a finely turned system for modulating available E7 and E2 proteins.

CK2 phosphorylation of the PRH/Hex homeodomain functions as a reversible switch for DNA binding (2009)
Journal Article
Soufi, A., Noy, P., Buckle, M., Sawasdichai, A., Gaston, K., & Jayaraman, P. (2009). CK2 phosphorylation of the PRH/Hex homeodomain functions as a reversible switch for DNA binding. Nucleic Acids Research, 37(10), 3288-3300. doi:10.1093/nar/gkp197

The proline-rich homeodomain protein (PRH/Hex) regulates transcription by binding to specific DNA sequences and regulates mRNA transport by binding to translation initiation factor eIF4E. Protein kinase CK2 plays multiple roles in the regulation of g... Read More about CK2 phosphorylation of the PRH/Hex homeodomain functions as a reversible switch for DNA binding.

The regulation of cell proliferation by the papillomavirus early proteins (2009)
Journal Article
Abdul Hamid, N., Brown, C., & Gaston, K. (2009). The regulation of cell proliferation by the papillomavirus early proteins. Cellular and Molecular Life Sciences, 66(10), 1700-1717. doi:10.1007/s00018-009-8631-7

The human papillomavirus (HPV) E6 and E7 oncogenes have direct effects on host cell proliferation. The viral E2 protein regulates transcription of E6 and E7 and thereby has an indirect effect on cell proliferation. In HPV-induced tumours, misappropri... Read More about The regulation of cell proliferation by the papillomavirus early proteins.

The PRH/Hex repressor protein causes nuclear retention of Groucho/TLE co-repressors (2008)
Journal Article
Desjobert, C., Noy, P., Swingler, T., Williams, H., Gaston, K., & Jayaraman, P. (2009). The PRH/Hex repressor protein causes nuclear retention of Groucho/TLE co-repressors. BBA - Biomembranes, 417(1), 121-132. doi:10.1042/BJ20080872

The PRH (proline-rich homeodomain) [also known as Hex (haematopoietically expressed homeobox)] protein is a transcription factor that functions as an important regulator of vertebrate development and many other processes in the adult including haemat... Read More about The PRH/Hex repressor protein causes nuclear retention of Groucho/TLE co-repressors.

DNA wrapping and distortion by an oligomeric homeodomain protein (2008)
Journal Article
Williams, H., Jayaraman, P., & Gaston, K. (2008). DNA wrapping and distortion by an oligomeric homeodomain protein. Journal of Molecular Biology, 383, 10-23. doi:10.1016/j.jmb.2008.08.004

Many transcription factors alter DNA or chromatin structure. Changes in chromatin structure are often brought about by the recruitment of chromatin-binding proteins, chromatin-modifying proteins, or other transcription co-activator or co-repressor pr... Read More about DNA wrapping and distortion by an oligomeric homeodomain protein.

The papillomavirus E2 DNA binding domain (2008)
Journal Article
de Prat-Gay, G., Gaston, K., & Cicero, D. O. (2008). The papillomavirus E2 DNA binding domain. Frontiers in Bioscience, 13, 6006-6021. doi:10.2741/3132

The DNA binding domain of the E2 master regulator from papillomaviruses is the primary effector for most the essential activities controlled by this protein. In this review we focus on the properties of the DNA binding domain of human papillomavirus... Read More about The papillomavirus E2 DNA binding domain.

p53 represses human papillomavirus type 16 DNA replication via the viral E2 protein (2008)
Journal Article
Brown, C., Kowalczyk, A. M., Taylor, E. R., Morgan, I. M., & Gaston, K. (2008). p53 represses human papillomavirus type 16 DNA replication via the viral E2 protein. Virology Journal, 5, doi:10.1186/1743-422X-5-5

Background. Human papillomavirus (HPV) DNA replication can be inhibited by the cellular tumour suppressor protein p53. However, the mechanism through which p53 inhibits viral replication and the role that this might play in the HPV life cycle are not... Read More about p53 represses human papillomavirus type 16 DNA replication via the viral E2 protein.

Comprehensive comparison of the interaction of the E2 master regulator with its cognate target DNA sites in 73 human papillomavirus types by sequence statistics (2007)
Journal Article
Sánchez, I. E., Dellarole, M., Gaston, K., & de Prat Gay, G. (2008). Comprehensive comparison of the interaction of the E2 master regulator with its cognate target DNA sites in 73 human papillomavirus types by sequence statistics. Nucleic Acids Research, 36(3), 756-69. https://doi.org/10.1093/nar/gkm1104

Mucosal human papillomaviruses (HPVs) are etiological agents of oral, anal and genital cancer. Properties of high- and low-risk HPV types cannot be reduced to discrete molecular traits. The E2 protein regulates viral replication and transcription thr... Read More about Comprehensive comparison of the interaction of the E2 master regulator with its cognate target DNA sites in 73 human papillomavirus types by sequence statistics.

A cancer cell-specific inducer of apoptosis (2007)
Journal Article
Green, K. L., Brown, C., Roeder, G. E., Southgate, T. D., & Gaston, K. (2007). A cancer cell-specific inducer of apoptosis. Human Gene Therapy, 18(6), 547-561. doi:10.1089/hum.2006.042

Human papillomavirus (HPV) DNA is found in virtually all cervical cancers, strongly suggesting that these viruses are necessary to initiate this disease. The HPV E2 protein is required for viral replication, but E2 expression is usually lost in HPV-t... Read More about A cancer cell-specific inducer of apoptosis.

Characterization of an enhancer region of the galanin gene that directs expression to the dorsal root ganglion and confers responsiveness to axotomy (2007)
Journal Article
Bacon, A., Kerr, N. C., Holmes, F. E., Gaston, K., & Wynick, D. (2007). Characterization of an enhancer region of the galanin gene that directs expression to the dorsal root ganglion and confers responsiveness to axotomy. Journal of Neuroscience, 27(24), 6573-6580. doi:10.1523/JNEUROSCI.1596-07.2007

Galanin expression markedly increases in the dorsal root ganglion (DRG) after sciatic nerve axotomy and modulates pain behavior and regeneration of sensory neurons. Here, we describe transgenic mice expressing constructs with varying amounts of seque... Read More about Characterization of an enhancer region of the galanin gene that directs expression to the dorsal root ganglion and confers responsiveness to axotomy.

A quadrivalent vaccine for human papillomavirus (2006)
Journal Article
Brown, C., & Gaston, K. (2006). A quadrivalent vaccine for human papillomavirus. Drugs of today (Barcelona, Spain : 1998), 42(11), 703-709. doi:10.1358/dot.2006.42.11.1032056

Cervical cancer is responsible for at least 13,500 deaths a year in the European Union and more than 30 times that worldwide. DNA from a group of human papillomaviruses (HPVs) is found in virtually all cervical carcinoma cells, and these so-called hi... Read More about A quadrivalent vaccine for human papillomavirus.

The recognition of local DNA conformation by the human papillomavirus type 6 E2 protein (2006)
Journal Article
Hooley, E., Fairweather, V., Clarke, A. R., Gaston, K., & Brady, R. L. (2006). The recognition of local DNA conformation by the human papillomavirus type 6 E2 protein. Nucleic Acids Research, 34(14), 3897-3908. doi:10.1093/nar/gkl466

The E2 proteins are transcription/replication factors from papillomaviruses. Human papillomaviruses (HPVs) can be broadly divided in two groups; low-risk HPV subtypes cause benign warts while high-risk HPVs give rise to cervical cancer. Although a ra... Read More about The recognition of local DNA conformation by the human papillomavirus type 6 E2 protein.