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The proline rich homeodomain protein PRH/Hhex forms stable oligomers that are highly resistant to denaturation

Shukla, Anshuman; Burton, Nicholas M.; Jayaraman, Padma-Sheela; Gaston, Kevin

Authors

Anshuman Shukla

Nicholas M. Burton

Padma-Sheela Jayaraman

Kevin Gaston



Abstract

Background: Many transcription factors control gene expression by binding to specific DNA sequences at or near the genes that they regulate. However, some transcription factors play more global roles in the control of gene expression by altering the architecture of sections of chromatin or even the whole genome. The ability to form oligomeric protein assemblies allows many of these proteins to manipulate extensive segments of DNA or chromatin via the formation of structures such as DNA loops or protein-DNA fibres. Principal Findings: Here we show that the proline rich homeodomain protein PRH/Hhex forms predominantly octameric and/or hexadecameric species in solution as well as larger assemblies. We show that these assemblies are highly stable resisting denaturation by temperature and chemical denaturants. Conclusion: These data indicate that PRH is functionally and structurally related to the Lrp/AsnC family of proteins, a group of proteins that are known to act globally to control gene expression in bacteria and archaea.

Journal Article Type Article
Publication Date 2012-04
Journal PLoS ONE
Publisher Public Library of Science
Peer Reviewed Peer Reviewed
Volume 7
Issue 4
Article Number e35984
Pages e35984 -- e35984
APA6 Citation Shukla, A., Burton, N. M., Jayaraman, P., & Gaston, K. (2012). The proline rich homeodomain protein PRH/Hhex forms stable oligomers that are highly resistant to denaturation. PLoS ONE, 7(4), doi:10.1371/journal.pone.0035984
DOI https://doi.org/10.1371/journal.pone.0035984
Publisher URL https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0035984

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