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All Outputs (16)

Functional modulation of PTH1R activation and signaling by RAMP2 (2022)
Journal Article
Nemec, K., Schihada, H., Kleinau, G., Zabel, U., Grushevskyi, E. O., Scheerer, P., Lohse, M. J., & Maiellaro, I. (2022). Functional modulation of PTH1R activation and signaling by RAMP2. Proceedings of the National Academy of Sciences, 119(32), Article e2122037119. https://doi.org/10.1073/pnas.2122037119

Receptor-activity-modifying proteins (RAMPs) are ubiquitously expressed membrane proteins that associate with different G protein-coupled receptors (GPCRs), including the parathyroid hormone 1 receptor (PTH1R), a class B GPCR and an important modulat... Read More about Functional modulation of PTH1R activation and signaling by RAMP2.

Receptor-associated independent cAMP nanodomains mediate spatiotemporal specificity of GPCR signaling (2022)
Journal Article
Anton, S. E., Kayser, C., Maiellaro, I., Nemec, K., Möller, J., Koschinski, A., Zaccolo, M., Annibale, P., Falcke, M., Lohse, M. J., & Bock, A. (2022). Receptor-associated independent cAMP nanodomains mediate spatiotemporal specificity of GPCR signaling. Cell, 185(7), 1130-1142.e11. https://doi.org/10.1016/j.cell.2022.02.011

G protein-coupled receptors (GPCRs) relay extracellular stimuli into specific cellular functions. Cells express many different GPCRs, but all these GPCRs signal to only a few second messengers such as cAMP. It is largely unknown how cells distinguish... Read More about Receptor-associated independent cAMP nanodomains mediate spatiotemporal specificity of GPCR signaling.

In Vivo cAMP Dynamics in Drosophila Larval Neurons (2022)
Book Chapter
Maiellaro, I. (2022). In Vivo cAMP Dynamics in Drosophila Larval Neurons. In M. Zaccolo (Ed.), cAMP Signaling: Methods and Protocols (181-194). Humana Press. https://doi.org/10.1007/978-1-0716-2245-2_11

Cyclic adenosine monophosphate (cAMP) is a universal second messenger that mediates a myriad of cell functions across all kingdoms of life. The ability to monitor intracellular changes of cAMP concentration in living cells using FRET-based biosensors... Read More about In Vivo cAMP Dynamics in Drosophila Larval Neurons.

Optical Mapping of cAMP Signaling at the Nanometer Scale (2020)
Journal Article
Bock, A., Annibale, P., Konrad, C., Hannawacker, A., Anton, S. E., Maiellaro, I., Zabel, U., Sivaramakrishnan, S., Falcke, M., & Lohse, M. J. (2020). Optical Mapping of cAMP Signaling at the Nanometer Scale. Cell, 182(6), 1519-1530.e17. https://doi.org/10.1016/j.cell.2020.07.035

Cells relay a plethora of extracellular signals to specific cellular responses by using only a few second messengers, such as cAMP. To explain signaling specificity, cAMP-degrading phosphodiesterases (PDEs) have been suggested to confine cAMP to dist... Read More about Optical Mapping of cAMP Signaling at the Nanometer Scale.

A universal bioluminescence resonance energy transfer sensor design enables high-sensitivity screening of GPCR activation dynamics (2018)
Journal Article
Schihada, H., Vandenabeele, S., Zabel, U., Frank, M., Lohse, M. J., & Maiellaro, I. (2018). A universal bioluminescence resonance energy transfer sensor design enables high-sensitivity screening of GPCR activation dynamics. Communications Biology, 1, Article 105. https://doi.org/10.1038/s42003-018-0072-0

G-protein-coupled receptors (GPCRs) represent one of the most important classes of drug targets. The discovery of new GCPR therapeutics would greatly benefit from the development of a generalizable high-throughput assay to directly monitor their acti... Read More about A universal bioluminescence resonance energy transfer sensor design enables high-sensitivity screening of GPCR activation dynamics.

Mechano-dependent signaling by Latrophilin/CIRL quenches cAMP in proprioceptive neurons (2017)
Journal Article
Scholz, N., Guan, C., Nieberler, M., Grotemeyer, A., Maiellaro, I., Gao, S., Beck, S., Pawlak, M., Sauer, M., Asan, E., Rothemund, S., Winkler, J., Prömel, S., Nagel, G., Langenhan, T., & Kittel, R. J. (2017). Mechano-dependent signaling by Latrophilin/CIRL quenches cAMP in proprioceptive neurons. eLife, 6, Article e28360. https://doi.org/10.7554/elife.28360

Adhesion-type G protein-coupled receptors (aGPCRs), a large molecule family with over 30 members in humans, operate in organ development, brain function and govern immunological responses. Correspondingly, this receptor family is linked to a multitud... Read More about Mechano-dependent signaling by Latrophilin/CIRL quenches cAMP in proprioceptive neurons.

Experimental and mathematical analysis of cAMP nanodomains (2017)
Journal Article
Lohse, C., Bock, A., Maiellaro, I., Hannawacker, A., Schad, L. R., Lohse, M. J., & Bauer, W. R. (2017). Experimental and mathematical analysis of cAMP nanodomains. PLoS ONE, 12(4), Article e0174856. https://doi.org/10.1371/journal.pone.0174856

In their role as second messengers, cyclic nucleotides such as cAMP have a variety of intracellular effects. These complex tasks demand a highly organized orchestration of spatially and temporally confined cAMP action which should be best achieved by... Read More about Experimental and mathematical analysis of cAMP nanodomains.

cAMP Signals in Drosophila Motor Neurons Are Confined to Single Synaptic Boutons (2016)
Journal Article
Maiellaro, I., Lohse, M., Kittel, R., & Calebiro, D. (2016). cAMP Signals in Drosophila Motor Neurons Are Confined to Single Synaptic Boutons. Cell Reports, 17(5), 1238-1246. https://doi.org/10.1016/j.celrep.2016.09.090

The second messenger cyclic AMP (cAMP) plays an important role in synaptic plasticity. Although there is evidence for local control of synaptic transmission and plasticity, it is less clear whether a similar spatial confinement of cAMP signaling exis... Read More about cAMP Signals in Drosophila Motor Neurons Are Confined to Single Synaptic Boutons.

Model Organisms in G Protein–Coupled Receptor Research (2015)
Journal Article
Langenhan, T., Barr, M. M., Bruchas, M. R., Ewer, J., Griffith, L. C., Maiellaro, I., Taghert, P. H., White, B. H., & Monk, K. R. (2015). Model Organisms in G Protein–Coupled Receptor Research. Molecular Pharmacology, 88(3), 596-603. https://doi.org/10.1124/mol.115.098764

The study of G protein–coupled receptors (GPCRs) has benefited greatly from experimental approaches that interrogate their functions in controlled, artificial environments. Working in vitro, GPCR receptorologists discovered the basic biologic mechani... Read More about Model Organisms in G Protein–Coupled Receptor Research.

Real-Time Monitoring of GPCR/cAMP Signalling by FRET and Single-Molecule Microscopy (2014)
Journal Article
Calebiro, D., Sungkaworn, T., & Maiellaro, I. (2014). Real-Time Monitoring of GPCR/cAMP Signalling by FRET and Single-Molecule Microscopy. Hormone and Metabolic Research, 46(12), 827-832. https://doi.org/10.1055/s-0034-1384523

G-protein-coupled receptors (GPCRs), located on the surface of virtually every cell in our organism, mediate the effects of many hormones and neurotransmitters. Although GPCRs have been extensively studied for more than 4 decades using pharmacologica... Read More about Real-Time Monitoring of GPCR/cAMP Signalling by FRET and Single-Molecule Microscopy.

Kinetics and mechanism of G protein-coupled receptor activation (2013)
Journal Article
Lohse, M. J., Maiellaro, I., & Calebiro, D. (2014). Kinetics and mechanism of G protein-coupled receptor activation. Current Opinion in Cell Biology, 27, 87-93. https://doi.org/10.1016/j.ceb.2013.11.009

The activation of a G protein-coupled receptor is generally triggered by binding of an agonist to the receptor's binding pocket, or, in the case of rhodopsin, by light-induced changes of the pre-bound retinal. This is followed by a series of a confor... Read More about Kinetics and mechanism of G protein-coupled receptor activation.

Termination and activation of store-operated cyclic AMP production (2012)
Journal Article
Maiellaro, I., Lefkimmiatis, K., Moyer, M. P., Curci, S., & Hofer, A. M. (2012). Termination and activation of store-operated cyclic AMP production. Journal of Cellular and Molecular Medicine, 16(11), 2715-2725. https://doi.org/10.1111/j.1582-4934.2012.01592.x

Diverse pathophysiological processes (e.g. obesity, lifespan determination, addiction and male fertility) have been linked to the expression of specific isoforms of the adenylyl cyclases (AC1‐AC10), the enzymes that generate cyclic AMP (cAMP). Our la... Read More about Termination and activation of store-operated cyclic AMP production.

Pseudomonas aeruginosaHomoserine Lactone Activates Store-operated cAMP and Cystic Fibrosis Transmembrane Regulator-dependent Cl−Secretion by Human Airway Epithelia (2010)
Journal Article
Schwarzer, C., Wong, S., Shi, J., Matthes, E., Illek, B., Ianowski, J. P., Arant, R. J., Isacoff, E., Vais, H., Foskett, J. K., Maiellaro, I., Hofer, A. M., & Machen, T. E. (2010). Pseudomonas aeruginosaHomoserine Lactone Activates Store-operated cAMP and Cystic Fibrosis Transmembrane Regulator-dependent Cl−Secretion by Human Airway Epithelia. Journal of Biological Chemistry, 285(45), 34850-34863. https://doi.org/10.1074/jbc.m110.167668

The ubiquitous bacterium Pseudomonas aeruginosa frequently causes hospital-acquired infections. P. aeruginosa also infects the lungs of cystic fibrosis (CF) patients and secretes N-(3-oxo-dodecanoyl)-S-homoserine lactone (3O-C12) to regulate bacteria... Read More about Pseudomonas aeruginosaHomoserine Lactone Activates Store-operated cAMP and Cystic Fibrosis Transmembrane Regulator-dependent Cl−Secretion by Human Airway Epithelia.

Store-operated cyclic AMP signalling mediated by STIM1 (2009)
Journal Article
Lefkimmiatis, K., Srikanthan, M., Maiellaro, I., Moyer, M. P., Curci, S., & Hofer, A. M. (2009). Store-operated cyclic AMP signalling mediated by STIM1. Nature Cell Biology, 11(4), 433-442. https://doi.org/10.1038/ncb1850

Depletion of Ca2+ from the endoplasmic reticulum (ER) results in activation of plasma membrane Ca2+ entry channels. This 'store-operated' process requires translocation of a transmembrane ER Ca2+ sensor protein, stromal interaction molecule 1 (STIM1)... Read More about Store-operated cyclic AMP signalling mediated by STIM1.