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In Vivo cAMP Dynamics in Drosophila Larval Neurons

Maiellaro, Isabella



Manuela Zaccolo


Cyclic adenosine monophosphate (cAMP) is a universal second messenger that mediates a myriad of cell functions across all kingdoms of life. The ability to monitor intracellular changes of cAMP concentration in living cells using FRET-based biosensors is proving to be of paramount importance to unraveling the sophisticated organization of cAMP signaling. Here we describe the deployment of the fruit fly Drosophila melanogaster, specifically the third instar larval stage, as an in vivo model to study the spatio-temporal dynamics of cAMP in neurons. The ubiquity of cAMP signaling and conservation of fundamental mechanisms across species ensures relevance to vertebrate neurons while providing a more structurally and ethically simple model.

Online Publication Date Mar 12, 2022
Publication Date Jan 1, 2022
Deposit Date Sep 21, 2022
Publisher Springer US
Pages 181-194
Series Title Methods in Molecular Biology
Series Number 2483
Book Title cAMP Signaling: Methods and Protocols
ISBN 9781071622445
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