cAMP Signals in Drosophila Motor Neurons Are Confined to Single Synaptic Boutons

Maiellaro, Isabella; Lohse, Martin�J.; Kittel, Robert�J.; Calebiro, Davide

Abstract

The second messenger cyclic AMP (cAMP) plays an important role in synaptic plasticity. Although there is evidence for local control of synaptic transmission and plasticity, it is less clear whether a similar spatial confinement of cAMP signaling exists. Here, we suggest a possible biophysical basis for the site-specific regulation of synaptic plasticity by cAMP, a highly diffusible small molecule that transforms the physiology of synapses in a local and specific manner. By exploiting the octopaminergic system of Drosophila, which mediates structural synaptic plasticity via a cAMP-dependent pathway, we demonstrate the existence of local cAMP signaling compartments of micrometer dimensions within single motor neurons. In addition, we provide evidence that heterogeneous octopamine receptor localization, coupled with local differences in phosphodiesterase activity, underlies the observed differences in cAMP signaling in the axon, cell body, and boutons.

Citation

Maiellaro, I., Lohse, M., Kittel, R., & Calebiro, D. (2016). cAMP Signals in Drosophila Motor Neurons Are Confined to Single Synaptic Boutons. Cell Reports, 17(5), 1238-1246. https://doi.org/10.1016/j.celrep.2016.09.090

• Journal Article Type: Article
• Acceptance Date: Aug 27, 2017
• Online Publication Date: Oct 25, 2016
• Publication Date: Oct 25, 2016
• Deposit Date: Apr 8, 2020
• Publicly Available Date: Apr 28, 2020
• Journal: Cell Reports
• Print ISSN: 2211-1247
• Electronic ISSN: 2211-1247
• Publisher: Cell Press
• Peer Reviewed: Peer Reviewed
• Volume: 17
• Issue: 5
• Pages: 1238-1246
• DOI: https://doi.org/10.1016/j.celrep.2016.09.090
• Public URL: https://nottingham-repository.worktribe.com/output/4237359
• Publisher URL: https://www.cell.com/cell-reports/fulltext/S2211-1247(16)31366-3?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS2211124716313663%3Fshowall%3Dtrue
• Additional Information: This article is maintained by: Elsevier; Article Title: cAMP Signals in Drosophila Motor Neurons Are Confined to Single Synaptic Boutons; Journal Title: Cell Reports; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.celrep.2016.09.090; Content Type: article; Copyright: © 2016 The Author(s).