Konstantinos Lefkimmiatis
Store-operated cyclic AMP signalling mediated by STIM1
Lefkimmiatis, Konstantinos; Srikanthan, Meera; Maiellaro, Isabella; Moyer, Mary Pat; Curci, Silvana; Hofer, Aldebaran M.
Authors
Meera Srikanthan
Dr ISABELLA MAIELLARO Isabella.Maiellaro@nottingham.ac.uk
Anne Mclaren Fellowship
Mary Pat Moyer
Silvana Curci
Aldebaran M. Hofer
Contributors
Dr ISABELLA MAIELLARO Isabella.Maiellaro@nottingham.ac.uk
Researcher
Abstract
Depletion of Ca2+ from the endoplasmic reticulum (ER) results in activation of plasma membrane Ca2+ entry channels. This 'store-operated' process requires translocation of a transmembrane ER Ca2+ sensor protein, stromal interaction molecule 1 (STIM1), to sites closely apposed to Ca2+ channels at the cell surface. However, it is not known whether a reduction in Ca2+ stores is coupled to other signalling pathways by this mechanism. We found that lowering the concentration of free Ca2+ in the ER, independently of the cytosolic Ca2+ concentration, also led to recruitment of adenylyl cyclases. This resulted in enhanced cAMP accumulation and PKA activation, measured using FRET-based cAMP indicators. Translocation of STIM1 was required for efficient coupling of ER Ca2+ depletion to adenylyl cyclase activity. We propose the existence of a pathway (store-operated cAMP signalling or SOcAMPS) in which the content of internal Ca2+ stores is directly connected to cAMP signalling through a process that involves STIM1.
Citation
Lefkimmiatis, K., Srikanthan, M., Maiellaro, I., Moyer, M. P., Curci, S., & Hofer, A. M. (2009). Store-operated cyclic AMP signalling mediated by STIM1. Nature Cell Biology, 11(4), 433-442. https://doi.org/10.1038/ncb1850
Journal Article Type | Article |
---|---|
Acceptance Date | Dec 5, 2008 |
Online Publication Date | Mar 15, 2009 |
Publication Date | 2009-04 |
Deposit Date | Apr 8, 2020 |
Journal | Nature Cell Biology |
Print ISSN | 1465-7392 |
Electronic ISSN | 1476-4679 |
Publisher | Nature Publishing Group |
Peer Reviewed | Peer Reviewed |
Volume | 11 |
Issue | 4 |
Pages | 433-442 |
DOI | https://doi.org/10.1038/ncb1850 |
Public URL | https://nottingham-repository.worktribe.com/output/4237837 |
Publisher URL | https://www.nature.com/articles/ncb1850 |
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