Real-Time Monitoring of GPCR/cAMP Signalling by FRET and Single-Molecule Microscopy

Calebiro, Davide; Sungkaworn, Titiwat; Maiellaro, Isabella

Abstract

G-protein-coupled receptors (GPCRs), located on the surface of virtually every cell in our organism, mediate the effects of many hormones and neurotransmitters. Although GPCRs have been extensively studied for more than 4 decades using pharmacological and biochemical methods, the recent introduction of optical methods such as fluorescence resonance energy transfer (FRET) and single-molecule microscopy is fostering novel and important discoveries in the field. Here, we review the use of such optical methods, focusing on some recent examples of their application to important and still unresolved questions concerning the spatial organisation and dynamics of GPCR signalling.

Citation

Calebiro, D., Sungkaworn, T., & Maiellaro, I. (2014). Real-Time Monitoring of GPCR/cAMP Signalling by FRET and Single-Molecule Microscopy. Hormone and Metabolic Research, 46(12), 827-832. https://doi.org/10.1055/s-0034-1384523

• Journal Article Type: Article
• Acceptance Date: Jun 17, 2014
• Online Publication Date: Jul 23, 2014
• Publication Date: 2014
• Deposit Date: Apr 8, 2020
• Journal: Hormone and Metabolic Research
• Print ISSN: 0018-5043
• Electronic ISSN: 1439-4286
• Publisher: Thieme Publishing
• Peer Reviewed: Peer Reviewed
• Volume: 46
• Issue: 12
• Pages: 827-832
• DOI: https://doi.org/10.1055/s-0034-1384523
• Keywords: Clinical Biochemistry; Biochemistry; Endocrinology, Diabetes and Metabolism; Endocrinology; Biochemistry, medical; General Medicine
• Public URL: https://nottingham-repository.worktribe.com/output/4237691
• Publisher URL: https://www.thieme-connect.de/products/ejournals/abstract/10.1055/s-0034-1384523